Tbcel (NM_001014089) Rat Tagged ORF Clone

CAT#: RR205905

Tbcel (Myc-DDK-tagged ORF) - Rat tubulin folding cofactor E-like (Tbcel), (10 ug)

Product Images

Circular map for RR205905

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tbcel
• Synonyms: Lrrc35; RGD1308880
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205905 representing NM_001014089
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCAGCCTAGTGGGAGAAGTTTCATGCAAGTATTGTGTGAAAAGTACAGTCCTGAAAACTTCCCTT
  ACCGCCGTGGTCCTGGGATGGGAGTCCATGTCCCAGCCACTCCTCAGGGCTCTCCTATGAAAGATCGCCT
  CAACCTCCCAAGCGTGCTCGTGCTGAACAGCTGCGGGATTACCTGTGCGGGAGACGAAAGAGAAATCGCA
  GCCTTCTGCGCTCACGTGTCAGAGCTCGATCTTTCTGACAACAAGCTCCAAGACTGGCATGAGGTCAGTA
  AGATTGTGTCAAACGTTCCTCAACTGGAGTTTCTCAACCTGAGTTCCAATCCTCTGAGTTTGTCAGTTTT
  AGAAAGAACATGCGCTGGGTCCTTCTCTGGGGTTCGCAAACTTGTCCTCAACAACAGCAAAGCATCATGG
  GAAACAGTGCACACAATACTGCAGGAGTTGCCAGATTTGGAGGAGCTCTTCCTGTGCCTTAATGACTATG
  AAACAGTGTCTTGTCCTTCTGTTTGCTGTCATTCCCTTAAGCTCCTACACATAACAGACAATAACCTCCA
  AGAGTGGACTGAAATCCGAAAGCTGGGTGTAATGTTCCCTTCGCTGGATACCCTGGTCCTGGCCAACAAT
  CACGTGAATGCCATCGAGGAGCCTGCTGACTCCCTGGCTAGGTTGTTCCCTAATCTGCGGTCCATCAGCC
  TCCACAAGTCAGGTTTGCAGTCCTGGGAAGACATTGACAAACTCAATTCATTCCCCAAACTCGAAGAAGT
  GAGATTGTTAGGAATCCCTCTCCTGCAGCCATATACCACCGAGGAGCGCAGGAAATTGGTGGTAGCCAGA
  TTGCCCTCCGTTTCCAAGCTCAATGGCAGTGTTGTGACGGATGGTGAGCGCGAGGATTCTGAGAGGTTCT
  TCATCCGGTACTATGTGGATGTTCCCCAGGAGGAAGTGCCATTCAGGTATCATGAGCTCATTACAAAGTA
  TGGGAAACTGGAGCCTTTGGCAGAAGTGGACCTGAGACCCCAGAGCAGTGCGAAAGTCGAAGTCCACTTC
  AACGACCAGGTGGAGGAAGTGAGCATCCGTCTGGACCAGACGGTGGCGGAGCTGAAGAGACAGCTGAAGA
  CTCTGGTGCAGCTGCCCACGAGCAGCATGCTCCTCTACTACTTTGACCACGAGGCCCCGTTTGGCCCGGA
  GGAGATGAAGTACAGCTCGCGAGCTCTGCACTCTTTCGGCATTAGGGATGGAGACAAAATTTTTGTGGAA
  TCAAAAACAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205905 representing NM_001014089
  Red=Cloning site Green=Tags(s)
  
  MDQPSGRSFMQVLCEKYSPENFPYRRGPGMGVHVPATPQGSPMKDRLNLPSVLVLNSCGITCAGDEREIA
  AFCAHVSELDLSDNKLQDWHEVSKIVSNVPQLEFLNLSSNPLSLSVLERTCAGSFSGVRKLVLNNSKASW
  ETVHTILQELPDLEELFLCLNDYETVSCPSVCCHSLKLLHITDNNLQEWTEIRKLGVMFPSLDTLVLANN
  HVNAIEEPADSLARLFPNLRSISLHKSGLQSWEDIDKLNSFPKLEEVRLLGIPLLQPYTTEERRKLVVAR
  LPSVSKLNGSVVTDGEREDSERFFIRYYVDVPQEEVPFRYHELITKYGKLEPLAEVDLRPQSSAKVEVHF
  NDQVEEVSIRLDQTVAELKRQLKTLVQLPTSSMLLYYFDHEAPFGPEEMKYSSRALHSFGIRDGDKIFVE
  SKTK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001014089
• ORF Size: 1272 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014089.1, NP_001014111.1
• RefSeq Size: 1566 bp
• RefSeq ORF: 1275 bp
• Locus ID: 315591
• UniProt ID: Q5PQJ7
• Cytogenetics: 8q22
• MW: 48 kDa
• Gene Summary: Acts as a regulator of tubulin stability.[UniProtKB/Swiss-Prot Function]