Hdac8 (NM_001126373) Rat Tagged ORF Clone

CAT#: RR205688

Hdac8 (Myc-DDK-tagged ORF) - Rat histone deacetylase 8 (Hdac8), (10 ug)

Product Images

Circular map for RR205688

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hdac8
• Synonyms: HD8; RGD1562895
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205688 representing NM_001126373
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATACCAGAGGAACCCGCCAATAGTGGGCATTCGCTACCCCCGGTTTATATTTACAGTCCCGAGT
  ATGTCAGCATCTGCGATTCCCTTGTGAAGGTCCCCAAACGGGCCAGTATGGTTCACTCTCTGATCGAAGC
  ATATGCCCTGCATAAACAAATGAGGATAGTGAAGCCCAAAGTGGCCTCCATGGAGGAGATGGCCACCTTC
  CACACTGATGCCTATCTTCAACATCTCCAGAAGGTCAGCCAAGAAGGGGATGAGGACCATCCAGACTCCA
  TAGAATATGGACTAGGTTATGACTGCCCAGCCACAGAAGGGATATTTGACTATGCAGCAGCTATAGGAGG
  AGGTACAATCACAGCTGCCCAGTGCCTGATTGACGGGAAGTGTAAAGTAGCCATTAACTGGTCTGGAGGG
  TGGCATCATGCAAAGAAAGATGAAGCATCTGGTTTCTGTTATCTCAATGATGCTGTCCTAGGAATATTAC
  GATTGCGACGGAAATTTGACCGGATTCTCTATGTGGACTTGGATCTACACCATGGAGATGGTGTAGAAGA
  TGCCTTCAGTTTTACATCTAAAGTTATGACTGTGTCCCTGCACAAGTTCTCTCCAGGATTTTTCCCAGGA
  ACAGGTGACATGTCTGATGTTGGCCTGGGGAAAGGACGGTACTACAGTGTCAATGTGCCCATTCAGGATG
  GCATCCAGGATGAGAAGTACTATCACATCTGTGAAAGTGTACTAAAGGAAGTATACCAGGCCTTCAATCC
  GAAGGCAGTGGTTCTACAGCTGGGAGCAGATACCATTGCTGGAGATCCAATGTGCTCCTTTAACATGACA
  CCAGTGGGAATTGGCAAGTGTCTGAAGTATGTCCTTCAGTGGCAGTTGGCAACTCTGATTTTAGGAGGAG
  GAGGCTACAACCTTGCCAACACGGCTCGTTGCTGGACATACTTGACCGGGGTCATCCTAGGGAAAACACT
  ATCCTCTGAGATCCCAGATCATGAGTTTTTCACAGCATATGGTCCTGATTATGTGCTGGAAATCACGCCA
  AGCTGCCGGCCAGACCGCAATGAGCCCCATCGAATCCAGCAAATCCTCAACTACATCAAAGGGAATCTGA
  AGCATGTGGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205688 representing NM_001126373
  Red=Cloning site Green=Tags(s)
  
  MEIPEEPANSGHSLPPVYIYSPEYVSICDSLVKVPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATF
  HTDAYLQHLQKVSQEGDEDHPDSIEYGLGYDCPATEGIFDYAAAIGGGTITAAQCLIDGKCKVAINWSGG
  WHHAKKDEASGFCYLNDAVLGILRLRRKFDRILYVDLDLHHGDGVEDAFSFTSKVMTVSLHKFSPGFFPG
  TGDMSDVGLGKGRYYSVNVPIQDGIQDEKYYHICESVLKEVYQAFNPKAVVLQLGADTIAGDPMCSFNMT
  PVGIGKCLKYVLQWQLATLILGGGGYNLANTARCWTYLTGVILGKTLSSEIPDHEFFTAYGPDYVLEITP
  SCRPDRNEPHRIQQILNYIKGNLKHVV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001126373
• ORF Size: 1131 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001126373.2, NP_001119845.2
• RefSeq Size: 2002 bp
• RefSeq ORF: 1134 bp
• Locus ID: 363481
• UniProt ID: B1WC68
• Cytogenetics: Xq22
• MW: 41.8 kDa
• Gene Summary: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility (By similarity).[UniProtKB/Swiss-Prot Function]