Iqgap1 (NM_001108489) Rat Tagged ORF Clone

CAT#: RR205329

Iqgap1 (Myc-DDK-tagged ORF) - Rat IQ motif containing GTPase activating protein 1 (Iqgap1), (10 ug)

Product Images

Circular map for RR205329

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Iqgap1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205329 representing NM_001108489
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGCCGCGGAGGAGGTCGATGGCCTGGGTGTGGTCCGGCCGCACTATGGCTCTGTCCTGGATAATG
  AGAGACTCACGGCAGAGGAGATGGATGAGAGGAGGCGACAGAACGTGGCCTATGAGTACCTTTGTCATTT
  GGAAGAAGCAAAGAGGTGGATGGAAGCATGCCTAGGGGAGGACCTGCCGCCCACCACAGAGCTGGAGGAG
  GGCCTTCGGAATGGGGTCTACCTTGCCAAGCTAGGGAACTTCTTCTCCCCCAAAGTGGTGTCCCTGAAGA
  AAATCTACGATCGAGAGCAGACCAGATACAAGGCTACCGGCCTACACTTCAGACACACGGATAATGTGAT
  TCAGTGGCTGAATGCCATGGATGAGATTGGATTGCCTAAGATTTTTTATCCAGAAACCACAGATATCTAC
  GACCGGAAGAACATGCCAAGATGTATCTACTGTATCCACGCCCTCAGTTTGTACCTGTTCAAACTGGGCC
  TGGCTCCTCAGATTCAAGACCTGTATGGAAAGGTTGACTTCACAGAGGAAGAAATCAACAACATGAAGAT
  TGAGCTTGAGAAGTACGGGATCCAGATGCCTGCCTTCAGCAAGATAGGGGGCATCCTGGCTAACGAGCTC
  TCGGTGGATGAGGCTGCATTACACGCTGCTGTCATTGCTATTAATGAAGCTATTGATCACAGAGTTGCAG
  CTGACACGTTTACGGCTCTGAAAAACCCCAATGCCATGCTTGTCAATCTTGAAGAAGCGCTGGCTCCCAC
  CTACCAGGATGTGCTTTACCAGGCCAAGCAGGACAAGATGACAAATGCTAAAAACAGGACGGAAAACTCT
  GACAGAGAAAGGGACGTTTATGAGGAACTGCTCACACAAGCTGAAATCCAAGGGAATGTAAACAAAGTCA
  ACACATCATCTGCCCTGGCCAACATCAACCTGGCTTTAGAGCAGGGCTGTGCACTGACCCTGCTCAAGGC
  TCTGCAGTCAGTGGCTCTGGGCCTCCGAGGGTTGCAGACCCAGAACAGCGACTGGTACCTGAAGCAGCTA
  CAGAGTGATCTGCAGCAAAAACGACAGAGTGGCCAGACTGACCCTCTGCAGAAGGAGGAGGTACAGGCCG
  GAGTGGATGCTGCCAACGGTGCTGCCCAACAATACCAACGACGGTTGGCAGCAGTGGCAGCGATCAATGC
  GGCGATCCAGAAGGGCATCGCCGAGAAGACCGTTTTGGAGCTAATGAATCCTGAAGCCCAGCTGCCCCAG
  GTGTATCCATTTGCAGCCGATCTTTATCAGAAGGAGTTGGCCACCCTGCAGCAGCAGAGTCCTGAGCATA
  GCCTCACCCATCCTGAACTCACTGTTGCCGTGGAGATGCTGTCTTCTGTGGCCCTCATCAACAGGGCGCT
  GGAGTCAGGAGACATGAACACGGTGTGGAAGCAGCTGAGCAGCTCCGTTACAGGCCTTACCAATATCGAG
  GAAGAAAACTGTCAAAGGTATCTGGATGAGCTGATGAAGCTGAAGGCGCAGGCACATGCAGAGAATAATG
  AATTTGTCACGTGGAATGACATCCAGGCGTGTGTGGACCACGTGAACCTGGTGGTCCACGAGGAACATGA
  GCGGATTTTGGCCATTGGTTTAATTAATGAAGCCCTGGATGAAGGGGACGCTCAGAAGACTCTGCAGGCC
  CTGCAGATCCCTGCAGCCAAGCTCGAGGGGGTCCTTCCAGAGGTGGCACAGCATTATCAAGACACGCTGA
  TCAGAGCAAAGAGAGAAAAGGCCCAGGAAACCCAGGATGAGTCAGCTGTGTTATGGTTGGATGAAATTCA
  AGGTGGAATCTGGCAGTCCAACAAAGACACCCAGGAAGCACAGAGGTTTGCCCTAGGAATCTTCGCCATC
  AACGAAGCAGTAGAGAGTGGTGATGTTGGCAGAACCCTGAGTGCCCTTCGTTCTCCGGACGTTGGCTTAT
  ATGGAGTGATCCCTGAGTGTGGGGAAACATACCAGAGTGACCTTGCTGAAGCCAAGAAGAAAAGACTAGC
  AGCAGGAGACAATAACAGCAAGTGGGTGAAGCACTGGGTGAAAGGCGGGTACCATTACTACCACAATCTG
  GAGACGCAAGCAGGAGGATGGGCTGAACCCCCAGACTTCGTGCAGAATTCTGTGCAGCTTTCTCGAGAGG
  AAATCCAGAGCTCCATCTCTGGAGTGACCGCTGCGTATAACCGAGAGCAGCTTTGGCTGGCCAACGAAGG
  CTTGATTACCAAGCTGCAAGCCTGCTGCCGTGGGTACCTTGTTCGACAGGAATTCCGATCCCGGATGAAC
  TTTCTGAAGAAACAGATCCCCGCCATCACCTGCATTCAGTCACAGTGGAGAGGATACAAACAGAAGAAGG
  CATATCAAGATCGGCTGGCTTACCTGCACTCCCATAAAGACGAAGTTGTGAAGATTCAGTCCCTTGCCAG
  GATGCATCAAGCCAGAAAGCGCTATAGAGATCGCCTACAGTATTTCCGAGACCACATAAATGACATTATC
  AAAATCCAGGCTTTCATTCGGGCAAACAAAGCTCGCGATGACTACAAGACTCTCATCAATGCTGAGGATC
  CGCCTATGATTGTGGTCCGAAAGTTTGTCCACCTCCTGGACCAAAGTGATCAGGACTTTCAGGAAGAGCT
  TGATCTTATGAAGATGCGGGAGGAGGTCATCACCCTCATCCGTTCCAACCAGCAGCTGGAGAATGACCTC
  AATCTCATGGATATCAAAATCGGCCTGCTGGTAAAGAACAAGATCACCCTGCAGGATGTGGTTTCCCATA
  GTAAAAAACTTACCAAAAAGAATAAGGAGCAGTTGTCTGACATGATGATGATAAACAAGCAGAAGGGCGG
  GCTCAAGGCCCTGAGCAAGGAGAAGAGGGAGAAGCTGGAAGCCTATCAGCATCTCTTCTATCTGCTACAG
  ACCAACCCTACCTATCTGGCCAAGCTGATTTTTCAGATGCCACAGAACAAGTCCACCAAGTTCATGGACT
  CTGTGATCTTCACGCTCTACAACTATGCATCCAACCAGCGGGAGGAGTACCTGCTGCTCCGACTCTTCCA
  GACGGCACTGCAGGAGGAGATCAAGTCAAAGGTGGATCAGATTCAAGAAATCGTGACAGGAAACCCTACA
  GTTATTAAGATGGTTGTAAGCTTCAACCGTGGTGCCCGGGGCCAGAATGCCCTCCGGCAGATCTTGGCTC
  CTGTTGTGAAGGAAATTATGGATGACAAGTCTCTCAACATCAAAACCGACCCCGTGGACATTTACAAGTC
  TTGGGTTAATCAGATGGAGTCTCAGACAGGAGAAGCGAGCAAACTGCCTTATGATGTGACCCCTGAGCAA
  GCCTTGTCTCATGAAGAAGTGAAGACGAGGTTAGACAACTCCATCAGGAACATGAGGGCTGTGACAGACA
  AGTTCCTTTCAGCCATCGTCAGCTCTGTGGACAAAATCCCTTACGGGATGCGCTTCATTGCCAAAGTGCT
  GAAGGACTCTTTGCACGAGAAGTTCCCGGATGCTGCCGAGGACGAGCTGCTGAAGATTATTGGTAACCTG
  CTTTACTACCGATACATGAACCCGGCCATCGTTGCTCCTGATGCCTTCGACATTATTGACCTGTCAGCAG
  GAGGCCAGCTCACCACAGACCAGCGTAGAAATCTGGGCTCCATTGCAAAGATGCTGCAGCACGCGGCTTC
  CAACAAGATGTTTCTGGGTGATAATGCTCACCTAAGCATCATTAATGAATATCTCTCTCAGTCCTACCAG
  AAATTCAGACGGTTTTTCCAAGCCGCTTGCGACGTCCCAGAGCTACAGGATAAGTTTAATGTGGATGAGT
  ATTCCGATCTAGTCACCCTCACCAAGCCGGTTATCTACATTTCCATTGGTGAGATCATCAACACCCACAC
  TCTCCTGTTGGACCATCAGGACGCCATTGCTCCAGAGCATAACGACCCCATCCATGAACTGCTGGATGAC
  CTTGGAGAGGTGCCCACCATTGAGTCCCTTATAGGAGAAAGCTGTGGTAATTCAAATGACCCCAGCAAGG
  AGGCGCTGGCTAAGACTGAAGTGTCTCTCACACTGACCAACAAGTTTGACGTGCCTGGTGATGAGAACGC
  AGAGATGGATGCGCGGACCATCTTACTGAATACAAAACGTTTAATTGTGGATGTCATCCGGTTCCAGCCA
  GGAGAGACCTTGACTGAAATCCTAGAAACCCCAGCCACCAATGAACAGGAAGCTGAACATCAGAGGGCCA
  TGCAGAGACGGGCTATCCGCGATGCCAAAACCCCTGACAAGATGAAAAAATCAAAGCCCATGAAGGAGGA
  TAACAACCTCAGCCTCCAGGAGAAGAAAGAGAAGATCCAGACTGGCCTAAAGAAGCTAACGGAACTTGGG
  ACGGTGGACCCAAAGAACAGATACCAGGAACTCATCAACGACATTGCTAAGGATATCCGGAATCAGCGGC
  GATACAGGCAGAGGAGGAAGGCTGAGCTGGTAAAGCTGCAGCAGACCTACTCGGCGCTGAACTCGAAGGC
  CACCTTTTACGGAGAGCAGGTGGATTACTACAAGAGCTACATCAAGACCTGCTTGGATAACTTGGCCAGC
  AAGGGCAAGGTCTCCAAAAAACCTAGGGAAATGAAAGGCAAAAAAAGCAAAAAGATTTCTCTGAAGTACA
  CAGCAGCAAGACTGCATGAGAAGGGAGTGCTTCTGGAAATTGAGGACCTTCAAGCAAACCAATTTAAAAA
  TGTTATCTTCGAAATTGGTCCAACAGAAGAAGTTGGAGACTTTGAAGTGAAGGCCAAGTTCATGGGCGTT
  CAGATGGAGACTTTCATGCTGCATTATCAGGACTTGCTGCAGCTACAGTATGAAGGGGTTGCGGTCATGA
  AGTTGTTTGATAGAGCTAAAGTGAATGTCAACCTCCTGATCTTCCTTCTCAACAAAAAGTTCTATGGGAA
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205329 representing NM_001108489
  Red=Cloning site Green=Tags(s)
  
  MSAAEEVDGLGVVRPHYGSVLDNERLTAEEMDERRRQNVAYEYLCHLEEAKRWMEACLGEDLPPTTELEE
  GLRNGVYLAKLGNFFSPKVVSLKKIYDREQTRYKATGLHFRHTDNVIQWLNAMDEIGLPKIFYPETTDIY
  DRKNMPRCIYCIHALSLYLFKLGLAPQIQDLYGKVDFTEEEINNMKIELEKYGIQMPAFSKIGGILANEL
  SVDEAALHAAVIAINEAIDHRVAADTFTALKNPNAMLVNLEEALAPTYQDVLYQAKQDKMTNAKNRTENS
  DRERDVYEELLTQAEIQGNVNKVNTSSALANINLALEQGCALTLLKALQSVALGLRGLQTQNSDWYLKQL
  QSDLQQKRQSGQTDPLQKEEVQAGVDAANGAAQQYQRRLAAVAAINAAIQKGIAEKTVLELMNPEAQLPQ
  VYPFAADLYQKELATLQQQSPEHSLTHPELTVAVEMLSSVALINRALESGDMNTVWKQLSSSVTGLTNIE
  EENCQRYLDELMKLKAQAHAENNEFVTWNDIQACVDHVNLVVHEEHERILAIGLINEALDEGDAQKTLQA
  LQIPAAKLEGVLPEVAQHYQDTLIRAKREKAQETQDESAVLWLDEIQGGIWQSNKDTQEAQRFALGIFAI
  NEAVESGDVGRTLSALRSPDVGLYGVIPECGETYQSDLAEAKKKRLAAGDNNSKWVKHWVKGGYHYYHNL
  ETQAGGWAEPPDFVQNSVQLSREEIQSSISGVTAAYNREQLWLANEGLITKLQACCRGYLVRQEFRSRMN
  FLKKQIPAITCIQSQWRGYKQKKAYQDRLAYLHSHKDEVVKIQSLARMHQARKRYRDRLQYFRDHINDII
  KIQAFIRANKARDDYKTLINAEDPPMIVVRKFVHLLDQSDQDFQEELDLMKMREEVITLIRSNQQLENDL
  NLMDIKIGLLVKNKITLQDVVSHSKKLTKKNKEQLSDMMMINKQKGGLKALSKEKREKLEAYQHLFYLLQ
  TNPTYLAKLIFQMPQNKSTKFMDSVIFTLYNYASNQREEYLLLRLFQTALQEEIKSKVDQIQEIVTGNPT
  VIKMVVSFNRGARGQNALRQILAPVVKEIMDDKSLNIKTDPVDIYKSWVNQMESQTGEASKLPYDVTPEQ
  ALSHEEVKTRLDNSIRNMRAVTDKFLSAIVSSVDKIPYGMRFIAKVLKDSLHEKFPDAAEDELLKIIGNL
  LYYRYMNPAIVAPDAFDIIDLSAGGQLTTDQRRNLGSIAKMLQHAASNKMFLGDNAHLSIINEYLSQSYQ
  KFRRFFQAACDVPELQDKFNVDEYSDLVTLTKPVIYISIGEIINTHTLLLDHQDAIAPEHNDPIHELLDD
  LGEVPTIESLIGESCGNSNDPSKEALAKTEVSLTLTNKFDVPGDENAEMDARTILLNTKRLIVDVIRFQP
  GETLTEILETPATNEQEAEHQRAMQRRAIRDAKTPDKMKKSKPMKEDNNLSLQEKKEKIQTGLKKLTELG
  TVDPKNRYQELINDIAKDIRNQRRYRQRRKAELVKLQQTYSALNSKATFYGEQVDYYKSYIKTCLDNLAS
  KGKVSKKPREMKGKKSKKISLKYTAARLHEKGVLLEIEDLQANQFKNVIFEIGPTEEVGDFEVKAKFMGV
  QMETFMLHYQDLLQLQYEGVAVMKLFDRAKVNVNLLIFLLNKKFYGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001108489
• ORF Size: 4971 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108489.1, NP_001101959.2
• RefSeq Size: 6158 bp
• RefSeq ORF: 4974 bp
• Locus ID: 361598
• Cytogenetics: 1q31
• MW: 188.8 kDa