Poli (NM_001106137) Rat Tagged ORF Clone

CAT#: RR204969

Poli (Myc-DDK-tagged ORF) - Rat polymerase (DNA directed), iota (Poli), (10 ug)

Product Images

Circular map for RR204969

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Poli
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204969 representing NM_001106137
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGTGGAGCCGGAGGAGGAAGGCGGCCCTGCGGAGGAAGAAGACTTTTCTCGTGCCATGGAGCCCT
  CGGACTCGGGGGCTGCGGGCGGCTCGCGGGCAGTTTGCGATCAAGGGGCACCATCACGAATCGCTTCGTC
  CAGAGTCATAGTACATGTGGACCTGGATTGTTTTTATGCCCAAGTAGAGATGATCTCCAATCCAGAATTA
  AAGGACAAACCTTTAGGTGTGCAACAGAAATACTTGGTGGTTACTTGCAACTATGAAGCCAGGAAACTTG
  GAGTGAAGAAGCTTATGAATGTCAGAGATGCAAAAGAAAAGTGTCCTCAGCTCGTCCTGGTGAATGGAGA
  AGACTTGAGCCGCTACCGGGAGATGTCCTATAAGGTCACAGAATTGTTGGAGGAATTTAGTCCAGCTGTG
  GAGAGGCTTGGATTTGATGAAAATTTTGTGGATCTGACAGAAATGGTTGAGAAAAGACTGCAGCAGCTTC
  CGAGTGACGAGGTTCCGTCAGTGACCATGTCCGGCCATGTTTACAATAACCAGTCTGTAAACCTGCACAG
  CAGCACGCATGTGAGACTTGCGGTTGGATCGCAGATTGCAGCAGAGATGCGGGAAGCCATGCATAACCAG
  CTGGGGCTCACAGGCTGCGCTGGCGTAGCGCCTAATAAACTATTGGCAAAGTTAGTGTCTGGTGTTTTTA
  AACCAAATCAACAGACAGTCTTATTACCTGAGAGTTGTCAGCATCTCATTCACAGTTTGAACCACATAAA
  GGAAATGCCTGGTATCGGCTATAAAACCGCCAAGCGTCTTGAAGTCCTGGGAATCAATAGTGTGCATGAT
  CTCCAGACCTTTCCAATCAAAACCTTAGAAAAGGAATTGGGAATTTCTGTTGCTCAGCGCATCCGGAAGC
  TCAGCTTTGGAGAGGACAAGTCGCCTGTAACACCCTCAGGGCCACCACAGTCCTTTAGTGAAGAAGATAC
  ATTTAAAAAGTGTTCCTCAGAAGTGGAAGCTAAAACTAAGATTGAAGAATTACTTTCCAGCCTTTTAAAC
  AGAGTATGCCATGATGGAAGGAAGCCCCATACAATAAGATTAGTCATCCGTCGATACTCTGACAAACACT
  GTAATCGAGAGAGTCGCCAGTGCCCAATCCCATCTCACGTCATTCAGAAGTTAGGGACAGGAAATTATGA
  TGCAGTGCCGCCCCTGATGGAGATCCTTATGAAACTTTTCCGAAATATGGTAAATGTGAAGATGGCCTTT
  CACCTGACCCTTCTGAGTGTGTGCTTCTGCAACCTGAAAGCCCTGAACAGTGCTAAGAAAGGGCCTATGG
  ACTGCTTCCTAACGTCGTCCCTGTCAACAACTGCCTGCTCTGGCAAGCGGAGCTTTAAAGTGAAGGACAT
  CCACATGGAGGACTCCTACAAGGAGAAAGAAGCAAACTGGGATTGTCTCCCAAGTAGAAGAATCGAAAGC
  ACGAGAAGGGGGGAGTCTCCATCGGATGCCACCTGCTTTCCTAAAGAAAAGGACACGAGTGACTTGCCAC
  TCCAGTTCCTGCCAGAGGGTGTTGACCAAGAAGTCTTCACGCAGCTTCCAGCAGATATTCAAGAAGAAAT
  CCTTTCTGGAAAATCTAGAGAAAATCTTCAAGGGAAAGGAAGTTTGAGTTGTCCGTTACATGCCTCGAGA
  GGAGTATTGTCTTTCTTTTCTACAAAGCAGACGCAGGCGGGCTGCTTAAGTCCCAGAGATACTCTGCTCA
  CTGGCAAGCAGGCATCGGCTGTGTCTCCCTGTGAGCCGGGAACGTCGGGGTTGAGTACCAGTAGCTCCTC
  CCATCCATCCTGCGGAAAGGACTGCTCCTATTATGTAGATAATCAGTTAAAGGACGAACGTATGAGTCAA
  GGCCCTACAGAGTCTCAGGGATTCCACTTTTCCAATACGAACCCTGCTGTTTCCGTTTTTCATTCATTTC
  CAAATCTGCAGAGTGAACAGCTTTTCTCCACACACCGCACTGCAGACAGCCACAAGCAGACAGCCACCAC
  CTCTCACCAGGGACTAGAAAACAGAGAGCAAGATTCTGCTGATGAAAAGCTTACTTTCCCATCGGACATT
  GATCCTCAGGTTTTCTATGAGCTACCAGAAGAGGTCCAAAGAGAACTGATGGCCGAATGGAAGAGAGCTG
  GGGCAGAGTTCCCCTCTGTGCACAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204969 representing NM_001106137
  Red=Cloning site Green=Tags(s)
  
  MGVEPEEEGGPAEEEDFSRAMEPSDSGAAGGSRAVCDQGAPSRIASSRVIVHVDLDCFYAQVEMISNPEL
  KDKPLGVQQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGEDLSRYREMSYKVTELLEEFSPAV
  ERLGFDENFVDLTEMVEKRLQQLPSDEVPSVTMSGHVYNNQSVNLHSSTHVRLAVGSQIAAEMREAMHNQ
  LGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNHIKEMPGIGYKTAKRLEVLGINSVHD
  LQTFPIKTLEKELGISVAQRIRKLSFGEDKSPVTPSGPPQSFSEEDTFKKCSSEVEAKTKIEELLSSLLN
  RVCHDGRKPHTIRLVIRRYSDKHCNRESRQCPIPSHVIQKLGTGNYDAVPPLMEILMKLFRNMVNVKMAF
  HLTLLSVCFCNLKALNSAKKGPMDCFLTSSLSTTACSGKRSFKVKDIHMEDSYKEKEANWDCLPSRRIES
  TRRGESPSDATCFPKEKDTSDLPLQFLPEGVDQEVFTQLPADIQEEILSGKSRENLQGKGSLSCPLHASR
  GVLSFFSTKQTQAGCLSPRDTLLTGKQASAVSPCEPGTSGLSTSSSSHPSCGKDCSYYVDNQLKDERMSQ
  GPTESQGFHFSNTNPAVSVFHSFPNLQSEQLFSTHRTADSHKQTATTSHQGLENREQDSADEKLTFPSDI
  DPQVFYELPEEVQRELMAEWKRAGAEFPSVHK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001106137
• ORF Size: 2196 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001106137.1, NP_001099607.1
• RefSeq Size: 2360 bp
• RefSeq ORF: 2199 bp
• Locus ID: 291526
• Cytogenetics: 18q12.1
• MW: 81.2 kDa