Rnpep (NM_031097) Rat Tagged ORF Clone

CAT#: RR204439

Rnpep (Myc-DDK-tagged ORF) - Rat arginyl aminopeptidase (aminopeptidase B) (Rnpep), (10 ug)

Product Images

Circular map for RR204439

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rnpep
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204439 representing NM_031097
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGCAGCGGACCCAGCAGCTGCCACAGCGCGGCCCGGCGGCCACTTCACTCGGCACAAGCCGTGG
  ACGTGGCCTCGGCCTCCAGTTTCCGCGCCTTCGAGATCCTGCACCTGCACCTGGACCTGCGGGCCGAGTT
  GCCTCCGGGCCCGGGTCCGGGGAGCCGCGGACTGAACGGCAAGGCGACGCTGGAACTGCGCTGCTTGCTG
  CCGGAGGGCGCCTCGGAGCTGCGGCTCGATTCGCACAGTTGCCTGGAGGTGATGGCGGCGACCCTGCTGC
  GGGGGCAGCCCGGAGACCAGCAGCAGCTCACGGAGCCCGTGCCTTTCCACACACAGCCCTTCTCGCACTA
  TGGCCAGGCCCTGTGCGTGGTCTTTCCGAAGCCCTGCTGTGCCGCCGAGCGCTTCCGGCTGGAGCTCACC
  TACCGCGTCGGAGAGGGACCAGGGGTTTGCTGGTTAGCTCCTGAGCAGACAGCAGGAAAGAAGAAACCCT
  TTGTGTATACCCAGGGTCAGGCAGTCTTAAACCGAGCCTTTTTCCCTTGCTTCGACACCCCAGCCGTGAA
  ATGCACGTATTCGGCTCTTGTTGAGGTTCCAGATGGCTTCACAGCTGTGATGAGTGCAAGCACATGGGAG
  AGGAGAGGACCAAATAAGTTCTTCTTCCAGATGAGCCAGCCCATCCCCTCCTATCTGATAGCTTTGGCCA
  TTGGAGATCTGGCTTCAGCTGAGGTTGGACCCAGGAGCCGGGTGACAGAGCCCTGCCTGATTGAAGCCGC
  CAAGGAGGAATACAATGGGGTCATAGAAGAATTTCTGGCAACAGGAGAGAAGCTTTTCGGACCCTATGTT
  TGGGGAAGATATGATTTACTCTTTATGCCACCATCTTTCCCGTTTGGAGGAATGGAGAATCCCTGCCTGA
  CCTTTGTCACCCCGTGCCTGCTAGCAGGGGACCGATCCTTAGCCGACGTCATCATCCACGAGATCTCCCA
  CAGTTGGTTTGGGAACCTGGTCACCAATGCCAACTGGGGGGAATTCTGGCTCAATGAAGGCTTCACCATG
  TATGCCCAGAGGAGGATCTCCACCATCCTGTTCGGAGCTGCTTACACTTGCTTGGAGGCCGCAACGGGGA
  GGGCTCTGCTACGGCAGCACATGGATGTGTCTGGAGAGGAAAACCCACTCAACAAGCTTCGGGTGAAGAT
  TGAACCAGGTGTTGACCCAGATGACACCTACAATGAAACCCCCTACGAGAAAGGTTACTGCTTTGTCTCA
  TACCTGGCCCATCTGGTGGGTGATCAGGAACAGTTTGACAAGTTTCTCAAGGCCTATGTAGATGAGTTTA
  AGTTCCAAAGTATCTTGGCTGAAGACTTTCTGGAATTCTACCTGGAGTATTTCCCTGAGCTGAAGAAGAA
  GGGAGTGGATTCCATCCCAGGTTTTGAGTTCAACCGATGGCTGAACACCCCTGGCTGGCCCCCCTACCTC
  CCCGACCTCTCCCCTGGGGACTCACTCATGAAGCCAGCTGAAGAGCTGGCGGAGCTGTGGGCAGCCTCAG
  AGCCAGACATGCAAGCCATCGAGGCTGTGGCCATCTCTACCTGGAAGACCTACCAGCTTGTCTACTTCCT
  AGATAAGATCCTGCAGAAGTCTCCTCTCCCTCCTGGGAACGTGAAAAAACTTGGGGAGACATACCCAAAG
  ATTTCCAATGCTCAGAATGCTGAGTTACGGCTAAGATGGGGCCAAATCATCCTTAAGAATGACCACCAAG
  AAGAGTTCTGGAAAGTGAAGGACTTCCTGCAGAGTCAGGGAAAGCAGAAGTACACTCTGCCACTGTACCA
  TGCCATGATGGGTGGCAGCGAGATGGCCCGGACCCTCGCCAAGGAGACCTTTTCAGCCACCGCTTCCCAG
  CTCCACAGTAACGTGGTCAACTATGTCCAGCAGATCCTGGCGCCCAAGGGCAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204439 representing NM_031097
  Red=Cloning site Green=Tags(s)
  
  MESSGPSSCHSAARRPLHSAQAVDVASASSFRAFEILHLHLDLRAELPPGPGPGSRGLNGKATLELRCLL
  PEGASELRLDSHSCLEVMAATLLRGQPGDQQQLTEPVPFHTQPFSHYGQALCVVFPKPCCAAERFRLELT
  YRVGEGPGVCWLAPEQTAGKKKPFVYTQGQAVLNRAFFPCFDTPAVKCTYSALVEVPDGFTAVMSASTWE
  RRGPNKFFFQMSQPIPSYLIALAIGDLASAEVGPRSRVTEPCLIEAAKEEYNGVIEEFLATGEKLFGPYV
  WGRYDLLFMPPSFPFGGMENPCLTFVTPCLLAGDRSLADVIIHEISHSWFGNLVTNANWGEFWLNEGFTM
  YAQRRISTILFGAAYTCLEAATGRALLRQHMDVSGEENPLNKLRVKIEPGVDPDDTYNETPYEKGYCFVS
  YLAHLVGDQEQFDKFLKAYVDEFKFQSILAEDFLEFYLEYFPELKKKGVDSIPGFEFNRWLNTPGWPPYL
  PDLSPGDSLMKPAEELAELWAASEPDMQAIEAVAISTWKTYQLVYFLDKILQKSPLPPGNVKKLGETYPK
  ISNAQNAELRLRWGQIILKNDHQEEFWKVKDFLQSQGKQKYTLPLYHAMMGGSEMARTLAKETFSATASQ
  LHSNVVNYVQQILAPKGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031097
• ORF Size: 1944 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031097.1, NP_112359.1
• RefSeq Size: 2208 bp
• RefSeq ORF: 1947 bp
• Locus ID: 81761
• UniProt ID: O09175
• Cytogenetics: 13q13
• MW: 72.4 kDa
• Gene Summary: binds zinc; catalyzes the release of N-terminal Arg and Lys from oligopeptides when P1' is not Pro [RGD, Feb 2006]