Clcn4 (NM_022198) Rat Tagged ORF Clone

CAT#: RR204393

Clcn4 (Myc-DDK-tagged ORF) - Rat chloride channel 4-2 (Clcn4-2), (10 ug)

Product Images

Circular map for RR204393

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Clcn4
• Synonyms: Clcn4-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204393 representing NM_022198
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGCCTCTGGGAACCTAATGGACTTCCTTGATGAGCCCTTCCCTGATGTGGGGACCTATGAAGACT
  TCCACACCATAGACTGGCTGAGGGAAAAGTCCCGGGACACTGACAGACATAGAAAGATAACCAGCAAAAG
  TAAAGAGTCTATTTGGGAGTTCATCAAGAGTCTGCTGGACGCATGGTCAGGATGGGTGGTGATGCTACTT
  ATTGGTCTGCTAGCAGGTACCCTGGCTGGAGTTATCGATCTCGCTGTTGACTGGATGACAGACCTCAAGG
  AGGGCGTCTGTCTGTCTGCATTCTGGTACAGCCATGAGCAATGCTGCTGGACCTCCAATGAGACCACTTT
  TGAGGACAGGGACAAGTGTCCCCTGTGGCAGAAGTGGTCAGAGCTTCTTCTGAGCCAGTCAGAGGGTGCT
  AGCGCTTACATTCTGAATTACTTAATGTACATTCTATGGGCATTGCTGTTTGCATTTCTGGCTGTCTCCC
  TGGTACGTGTGTTTGCACCATATGCCTGTGGCTCTGGCATACCTGAGATAAAGACTATTTTGAGTGGCTT
  TATCATCAGGGGCTACTTGGGGAAATGGACTCTTCTAATCAAGACTGTCACTCTCGTGCTGGTTGTATCC
  TCTGGCCTGAGTCTTGGGAAAGAGGGGCCACTGGTGCATGTGGCATGTTGCTGTGGCAACTTCTTTAGCA
  GCCTTTTCTCCAAGTATAGCAAGAATGAAGGCAAGAGGCGTGAGGTGCTTTCCGCTGCAGCTGCTGCTGG
  TGTCTCTGTGGCCTTTGGTGCTCCCATAGGAGGTGTACTCTTCAGTCTAGAGGAGGTCAGTTACTACTTT
  CCCTTGAAAACCTTGTGGAGGTCATTTTTTGCAGCCCTGGTGGCTGCCTTCACACTGCGCTCCATCAACC
  CCTTTGGAAATAGCCGCCTGGTTCTCTTTTATGTGGAGTATCATACACCTTGGTACATGGCTGAACTCTT
  CCCTTTCATCCTGCTTGGAGTCTTTGGGGGTTTGTGGGGAACCGTCTTCACACGCTGCAACATTGCCTGG
  TGCAGGAGGCGTAAAACCACCAGGCTGGGGAAGTATCCAGTGTTGGAGGTCATTGTGGTGACAGCCATCA
  CTGCCATCATTGCTTATCCCAATCCCTACACTCGCCAGAGCACCAGTGAGCTCATATCTGAGCTATTCAA
  TGATTGTGGGGCTCTTGAGTCTTCTCAGCTCTGTGACTACATCAATGACCCCAACATGACTCGGCCTGTG
  GATGACATTCCGGACCGGCCAGCTGGGGTTGGAGTTTACACAGCCATGTGGCAGCTGGCCTTGGCACTGA
  TCTTCAAAATAGTCATTACTATATTTACCTTTGGCATGAAGATTCCCTCAGGTCTCTTCATCCCCAGTAT
  GGCTGTAGGAGCCATGGCAGGCCGGATGGTGGGAATTGGTGTGGAGCAGCTGGCCTACCATCATCATGAC
  TGGATCATCTTCAGGAACTGGTGCAGGCCTGGAGCGGACTGTGTCACACCAGGGCTTTATGCGATGGTGG
  GAGCTGCAGCCTGTCTAGGTGGAGTGACTAGGATGACAGTGTCTCTGGTAGTCATCATGTTTGAACTGAC
  TGGAGGTCTGGAATATATTGTACCCCTGATGGCAGCTGCTGTTACCAGCAAGTGGGTGGCTGATGCCTTT
  GGGAAAGAAGGGATTTATGAAGCCCACATCCATCTGAATGGGTACCCATTTCTTGATGTGAAGGATGAGT
  TCACTCACCGTACCCTAGCCACTGATGTGATGCGGCCCCGCAGGGGAGAACCACCTTTGTCAGTACTAAC
  CCAGGACAGCATGACTGTGGAGGATGTGGAGACTCTCATAAAAGAGACAGACTACAATGGCTTTCCTGTG
  GTGGTCTCGAGAGATTCGGAGCGCCTCATAGGCTTTGCCCAGAGGCGGGAGCTAATCTTGGCTATAAAAA
  ATGCCAGACAGAGGCAAGAGGGCATCGTGAGCAATTCCATCATGTACTTCACAGAGGAGCCCCCTGAGCT
  GCCTGCCAACAGCCCACATCCACTGAAGCTGAGGCGCATTCTGAACCTGAGCCCTTTTACTGTCACAGAC
  CACACCCCCATGGAGACAGTGGTGGACATTTTCCGGAAACTGGGGCTCCGACAATGCCTGGTGACACGGA
  GTGGGAGGCTTCTTGGGATCATCACAAAAAAAGACGTTCTGAGACACATGGCGCAGATGGCAAACCAGGA
  CCCTGAATCCATCATGTTTAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204393 representing NM_022198
  Red=Cloning site Green=Tags(s)
  
  MSASGNLMDFLDEPFPDVGTYEDFHTIDWLREKSRDTDRHRKITSKSKESIWEFIKSLLDAWSGWVVMLL
  IGLLAGTLAGVIDLAVDWMTDLKEGVCLSAFWYSHEQCCWTSNETTFEDRDKCPLWQKWSELLLSQSEGA
  SAYILNYLMYILWALLFAFLAVSLVRVFAPYACGSGIPEIKTILSGFIIRGYLGKWTLLIKTVTLVLVVS
  SGLSLGKEGPLVHVACCCGNFFSSLFSKYSKNEGKRREVLSAAAAAGVSVAFGAPIGGVLFSLEEVSYYF
  PLKTLWRSFFAALVAAFTLRSINPFGNSRLVLFYVEYHTPWYMAELFPFILLGVFGGLWGTVFTRCNIAW
  CRRRKTTRLGKYPVLEVIVVTAITAIIAYPNPYTRQSTSELISELFNDCGALESSQLCDYINDPNMTRPV
  DDIPDRPAGVGVYTAMWQLALALIFKIVITIFTFGMKIPSGLFIPSMAVGAMAGRMVGIGVEQLAYHHHD
  WIIFRNWCRPGADCVTPGLYAMVGAAACLGGVTRMTVSLVVIMFELTGGLEYIVPLMAAAVTSKWVADAF
  GKEGIYEAHIHLNGYPFLDVKDEFTHRTLATDVMRPRRGEPPLSVLTQDSMTVEDVETLIKETDYNGFPV
  VVSRDSERLIGFAQRRELILAIKNARQRQEGIVSNSIMYFTEEPPELPANSPHPLKLRRILNLSPFTVTD
  HTPMETVVDIFRKLGLRQCLVTRSGRLLGIITKKDVLRHMAQMANQDPESIMFN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022198
• ORF Size: 2262 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022198.2, NP_071534.2
• RefSeq Size: 4415 bp
• RefSeq ORF: 2265 bp
• Locus ID: 60586
• Cytogenetics: Xq13
• MW: 84.4 kDa
• Gene Summary: may act as a chloride channel [RGD, Feb 2006]