Fermt2 (NM_001011915) Rat Tagged ORF Clone

CAT#: RR204382

Fermt2 (Myc-DDK-tagged ORF) - Rat fermitin family homolog 2 (Drosophila) (Fermt2), (10 ug)

Product Images

Circular map for RR204382

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fermt2
• Synonyms: Plekhc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204382 representing NM_001011915
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGGACGGGATAAGGATGCCAGATGGCTGCTACGCGGACGGGACGTGGGAACTGAGCGTCCATG
  TCACCGACCTGAACCGTGATGTCACCCTCAGAGTGACCGGGGAGGTGCACATCGGAGGGGTGATGCTGAA
  GTTGGTGGAAAAACTCGATGTCAAAAAAGATTGGTCTGACCATGCTCTCTGGTGGGAAAAGAAGAAAACT
  TGGCTGCTTAAGACACACTGGACCTTGGATAAGTATGGCATCCAGGCAGACGCCAAGCTTCAGTTCACCC
  CACAGCACAAACTGCTCCGCCTGCAGCTTCCCAACATGAAATATGTGAAGGTGAAAGTGAACTTCTCTGA
  CCGAGTCTTCAAAGCTGTGTCGGACATCTGCAAGACTTTCAATATAAGACACCCTGAAGAACTTTCTCTC
  TTAAAAAAACCCAGAGATCCCACGAAGAAGAAAAAGAAAAAGCTAGATGACCAGTCTGAAGATGAGGCAC
  TTGAATTGGAAGGACCTCTTATCATGCCTGGATCAGGAAGCATATACTCAAGCCCGGGACTTTATAGTAA
  AACAATGACCCCTACTTACGACGCTCACGATGGAAGCCCCTTGTCACCAACCTCCGCCTGGTTTGGTGAC
  AGTGCCCTGTCAGAAGGCAATCCCGGGATACTTGCTGTCAGTCAGCCGGTAACATCACCAGAAATACTGG
  CAAAAATGTTCAAGCCTCAAGCTCTTCTTGATAAAGCAAAAACCAACCAAGGGTGGCTGGATTCCTCAAG
  ATCTCTGATGGAGCAAGATGTGAAGGAGAACGAGGCCTTGCTGCTGCGATTCAAGTATTACAGCTTTTTC
  GATCTGAATCCAAAGTATGACGCCATCAGAATCAATCAGCTTTACGAGCAGGCCAAGTGGGCTCTCCTCC
  TAGAAGAGATCGAGTGCACGGAAGAAGAGATGATGATGTTCGCGGCCTTGCAGTATCATATCAATAAGCT
  GTCAATCATGACATCAGAAAATCATTTAAACAACAGTGACAAAGAAGTCGATGAAGTTGACGCCGCCCTT
  TCTGACCTGGAGATCACTTTGGAAGGCGGGAAAACATCAACAATCCTGGGTGACATTACTTCAATCCCAG
  AGCTTGCTGACTATATTAAAGTTTTCAAGCCTAAAAAGCTGACTTTAAAGGGGTACAAGCAGTACTGGTG
  CACCTTCAAAGACACGTCCATCTCCTGCTATAAGAGCCGAGAAGAGGCCAGTGGCACACCTGCTCACCAG
  ATGAACCTCAGAGGATGCGAAGTTACTCCGGATGTAAACATTTCGGGCCAGAAATTTAACATCAAACTCC
  TAATTCCGGTTGCAGAGGGCATGAACGAGATCTGGCTTCGCTGTGATAATGAGAAGCAGTATGCGCACTG
  GATGGCGGCCTGCAGACTGGCTTCCAAAGGCAAGACCATGGCAGACAGCTCTTACAACCTAGAAGTTCAG
  AACATCCTCTCCTTCCTGAAGATGCAACATCTGAACCCAGATCCTCAGCTTATCCCTGACCAGATCACAA
  CTGACATCAATCCCGAGTGCCTGGTGTCCCCGAGATACCTAAAAAAGTACAAGAACAAACAGATAACAGC
  ACGGATCTTGGAGGCCCACCAGAACGTAGCGCAGATGAGTCTGATCGAAGCCAAGATGAGATTCATTCAG
  GCGTGGCAGTCTCTGCCCGAGTTCGGCATCACACACTTCATCGCCAGATTTCAAGGCGGCAAGAGAGAAG
  AACTGATTGGAATTGCGTACAATAGATTGATTCGGATGGACGCCAGCACGGGAGACGCCATCAAAACATG
  GCGGTTCAGCAATATGAAGCAGTGGAATGTCAACTGGGAGATCAAAATGGTCACTGTAGAGTTTGCAGAC
  GAGGTCCGGTTGTCCTTCATCTGTACCGAAGTAGACTGCAAGGTGGTCCATGAATTCATTGGTGGCTACA
  TATTTCTCTCAACGCGTGCAAAAGACCAAAATGAGAGTTTAGATGAGGAGATGTTCTACAAACTCACCAG
  TGGTTGGGTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204382 representing NM_001011915
  Red=Cloning site Green=Tags(s)
  
  MALDGIRMPDGCYADGTWELSVHVTDLNRDVTLRVTGEVHIGGVMLKLVEKLDVKKDWSDHALWWEKKKT
  WLLKTHWTLDKYGIQADAKLQFTPQHKLLRLQLPNMKYVKVKVNFSDRVFKAVSDICKTFNIRHPEELSL
  LKKPRDPTKKKKKKLDDQSEDEALELEGPLIMPGSGSIYSSPGLYSKTMTPTYDAHDGSPLSPTSAWFGD
  SALSEGNPGILAVSQPVTSPEILAKMFKPQALLDKAKTNQGWLDSSRSLMEQDVKENEALLLRFKYYSFF
  DLNPKYDAIRINQLYEQAKWALLLEEIECTEEEMMMFAALQYHINKLSIMTSENHLNNSDKEVDEVDAAL
  SDLEITLEGGKTSTILGDITSIPELADYIKVFKPKKLTLKGYKQYWCTFKDTSISCYKSREEASGTPAHQ
  MNLRGCEVTPDVNISGQKFNIKLLIPVAEGMNEIWLRCDNEKQYAHWMAACRLASKGKTMADSSYNLEVQ
  NILSFLKMQHLNPDPQLIPDQITTDINPECLVSPRYLKKYKNKQITARILEAHQNVAQMSLIEAKMRFIQ
  AWQSLPEFGITHFIARFQGGKREELIGIAYNRLIRMDASTGDAIKTWRFSNMKQWNVNWEIKMVTVEFAD
  EVRLSFICTEVDCKVVHEFIGGYIFLSTRAKDQNESLDEEMFYKLTSGWV
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001011915
• ORF Size: 2040 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001011915.1, NP_001011915.1
• RefSeq Size: 3239 bp
• RefSeq ORF: 2043 bp
• Locus ID: 289992
• Cytogenetics: 15p14
• MW: 77.9 kDa