Ucp2 (NM_019354) Rat Tagged ORF Clone

CAT#: RR204365

Ucp2 (Myc-DDK-tagged ORF) - Rat uncoupling protein 2 (mitochondrial, proton carrier) (Ucp2), nuclear gene encoding mitochondrial protein, (10 ug)

Product Images

Circular map for RR204365

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ucp2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204365 representing NM_019354
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTGGTTTCAAGGCCACCGATGTGCCCCCCACAGCCACCGTGAAGTTCCTGGGGGCTGGGACAGCAG
  CCTGTATTGCAGATCTCATCACTTTCCCTCTAGACACCGCCAAAGTCCGGCTGCAGATCCAAGGAGAGAG
  TCAAGGGCTAGCGCGCACCGCCGCCAGCGCCCAGTACCGCGGCGTGCTGGGCACCATCCTAACCATGGTG
  CGCACTGAGGGTCCGCGCAGCCTCTACAATGGGCTGGTCGCCGGCCTACAGCGCCAGATGAGCTTTGCCT
  CCGTCCGCATTGGCCTCTACGACTCTGTAAAGCAGTTCTACACCAAGGGCTCAGAGCATGCAGGCATCGG
  GAGCCGCCTCCTGGCAGGTAGCACCACAGGTGCCCTGGCTGTGGCTGTGGCCCAACCTACAGATGTGGTA
  AAGGTCCGCTTCCAGGCCCAGGCCCGGGCTGGCGGTGGTCGGAGATACCAGAGCACTGTCGAAGCCTACA
  AGACCATTGCACGAGAGGAAGGGATCCGGGGCCTCTGGAAAGGGACCTCTCCCAATGTTGCCCGAAATGC
  CATTGTCAACTGTACTGAGCTGGTGACCTATGACCTCATCAAAGATACTCTCCTGAAAGCCAACCTCATG
  ACAGACGACCTCCCTTGCCACTTCACTTCTGCCTTCGGGGCGGGCTTCTGCACCACCGTCATTGCCTCCC
  CCGTTGATGTGGTCAAGACGAGATATATGAACTCTGCCTTGGGCCAGTACCACAGCGCCGGCCACTGTGC
  CCTGACCATGCTCCGGAAGGAGGGGCCCCGAGCCTTCTACAAGGGGTTCATGCCTTCCTTCCTCCGCTTG
  GGATCCTGGAACGTAGTAATGTTTGTCACCTATGAGCAGCTCAAAAGGGCCCTGATGGCTGCCTATGAAT
  CCCGGGAGGCACCCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204365 representing NM_019354
  Red=Cloning site Green=Tags(s)
  
  MVGFKATDVPPTATVKFLGAGTAACIADLITFPLDTAKVRLQIQGESQGLARTAASAQYRGVLGTILTMV
  RTEGPRSLYNGLVAGLQRQMSFASVRIGLYDSVKQFYTKGSEHAGIGSRLLAGSTTGALAVAVAQPTDVV
  KVRFQAQARAGGGRRYQSTVEAYKTIAREEGIRGLWKGTSPNVARNAIVNCTELVTYDLIKDTLLKANLM
  TDDLPCHFTSAFGAGFCTTVIASPVDVVKTRYMNSALGQYHSAGHCALTMLRKEGPRAFYKGFMPSFLRL
  GSWNVVMFVTYEQLKRALMAAYESREAPF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019354
• ORF Size: 927 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019354.3
• RefSeq Size: 930 bp
• RefSeq ORF: 930 bp
• Locus ID: 54315
• UniProt ID: P56500
• Cytogenetics: 1q32
• MW: 33.8 kDa
• Gene Summary: catalyzes a proton leak across the inner mitochondrial membrane to uncouple oxidative phosphorylation; may reduce the concentration of reactive ozygen species in mitochondria [RGD, Feb 2006]