Itgb1 (NM_017022) Rat Tagged ORF Clone

CAT#: RR204350

Itgb1 (Myc-DDK-tagged ORF) - Rat integrin beta 1 (fibronectin receptor beta) (Itgb1), (10 ug)

Product Images

Circular map for RR204350

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Itgb1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204350 representing NM_017022
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTTGCAACTGGTTTTCTGGATTGGATTGATCAGCTTGATTTGTTCTGTATTTGGCCAAACAGATA
  AAAATAGATGTTTAAAAGCAAATGCCAAATCTTGTGGAGAATGTATACAAGCAGGGCCAAATTGTGGGTG
  GTGTACAAATACGACATTTTTACAAGAAGGAATGCCTACTTCTGCACGATGTGATGATTTAGAAGCTTTG
  AAAAAGAAGGGTTGCCAACCAAGTGACATAGAGAATCCCAGAGGCTCCCAAACTATAAAGAAGAATAAAA
  ACGTCACCAATCGCAGCAAAGGGATGGCAGAGAAGCTCCGGCCAGAAGACATTACTCAGATCCAACCACA
  ACAGCTGCTTCTAAAGTTGCGATCAGGAGAACCACAGAAGTTTACATTAAAATTCAAGAGGGCTGAAGAC
  TACCCTATTGATCTGTACTACCTTATGGATCTCTCCTACTCTATGAAAGATGATCTGGAGAATGTGAAGA
  GTCTTGGGACGGATCTGATGAATGAAATGAGGAGGATTACTTCAGACTTCCGCATTGGCTTTGGCTCATT
  TGTGGAGAAAACTGTGATGCCATACATTAGTACAACACCAGCAAAGCTAAGAAATCCTTGTACGAGTGAA
  CAAAACTGTACCAGCCCATTTAGCTACAAAAACGTGCTTAGTCTTACTGACAGAGGAGAGTTTTTCAATG
  AACTTGTTGGTCAGCAGCGCATATCTGGAAACTTGGACTCTCCAGAAGGTGGCTTTGATGCAATCATGCA
  GGTTGCAGTTTGTGGGTCGCTGATTGGCTGGAGGAATGTAACACGACTGCTGGTGTTTTCCACGGATGCT
  GGGTTTCACTTTGCTGGAGATGGGAAACTTGGTGGTATTGTTTTACCCAATGATGGCCAGTGTCACCTGG
  AAAATAATGTGTATACAATGAGCCATTACTATGATTATCCTTCAATTGCTCACCTTGTTCAGAAACTAAG
  TGAAAATAATATTCAGACGATTTTTGCAGTCACTGAAGAGTTCCAGCCTGTTTACAAGGAATTGAAGAAT
  TTGATTCCTAAGTCAGCAGTGGGCACACTGTCTGGAAACTCTAGTAATGTGATACAGCTGATTATCGATG
  CCTACAATTCCCTTTCTTCAGAAGTCATTCTGGAAAACAGCAAATTGCCAGATGGAGTAACAATAAATTA
  CAAGTCCTACTGCAAGAATGGAGTGAATGGGACAGGAGAAAATGGACGAAAGTGCTCTAACATTTCCATT
  GGAGATGAGGTTCAATTTGAAATTAGCATAACTGCTAATAAATGTCCAAACAAGGAGTCTGAAACAATTA
  AAATTAAACCTCTGGGCTTCACAGAAGAAGTAGAGGTGGTCCTTCAGTTCATCTGTAAGTGTAATTGTCA
  AAGCCATGGCATCCCAGCAAGTCCCAAGTGCCATGAGGGAAATGGGACATTTGAGTGTGGAGCCTGCAGG
  TGCAATGAGGGTCGTGTTGGGAGACACTGTGAATGTAGCACAGATGAAGTGAACAGTGAAGACATGGATG
  CTTACTGCAGAAAAGAGAACAGCTCAGAGATCTGCAGTAACAATGGAGAGTGTGTCTGTGGACAGTGTGT
  GTGCAGGAAGAGAGAGAATACAAATGAAATTTACTCCGGCAAATTCTGCGAGTGTGATAACTTCAACTGC
  GATAGGTCCAACGGCTTAATCTGTGGAGGAAATGGTGTGTGCAGGTGTCGTGTCTGTGAATGCTATCCCA
  ACTACACTGGCAGTGCATGTGACTGTTCCTTGGACACTGTTCCATGCGTAGCGACAAATGGTCAGATCTG
  CAATGGCCGGGGCATCTGTGAATGTGGTGCTTGTAAGTGCACAGATCCCAAGTTCCAAGGGCCAACCTGT
  GAGACCTGTCAGACCTGCCTTGGTGTCTGTGCAGAGCATAAAGAATGTGTTCAGTGCAGAGCCTTCAATA
  AAGGAGAAAAAAAAGACACGTGTGCACAGGAGTGCTCCCATTTCAACCTCACTAAAGTGGAAAGCAGGGA
  GAAGTTGCCCCAGCCTGTGCAGGTGGACCCTGTGACCCACTGCAAGGAGAAGGACATTGATGACTGCTGG
  TTCTATTTCACCTACTCAGTGAACAGCAACGGTGAAGCTCACGTGCATGTTGTGGAGACTCCAGACTGTC
  CTACTGGTCCCGACATCATCCCAATTGTAGCAGGCGTGGTTGCCGGAATTGTTCTTATTGGCCTTGCCTT
  GCTGCTGATTTGGAAACTTTTAATGATAATTCATGACAGAAGGGAATTTGCTAAATTTGAAAAGGAGAAA
  ATGAATGCCAAGTGGGACACGGGTGAAAATCCTATTTACAAGAGTGCCGTGACAACTGTGGTCAATCCGA
  AGTATGAGGGAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204350 representing NM_017022
  Red=Cloning site Green=Tags(s)
  
  MNLQLVFWIGLISLICSVFGQTDKNRCLKANAKSCGECIQAGPNCGWCTNTTFLQEGMPTSARCDDLEAL
  KKKGCQPSDIENPRGSQTIKKNKNVTNRSKGMAEKLRPEDITQIQPQQLLLKLRSGEPQKFTLKFKRAED
  YPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSE
  QNCTSPFSYKNVLSLTDRGEFFNELVGQQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDA
  GFHFAGDGKLGGIVLPNDGQCHLENNVYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKN
  LIPKSAVGTLSGNSSNVIQLIIDAYNSLSSEVILENSKLPDGVTINYKSYCKNGVNGTGENGRKCSNISI
  GDEVQFEISITANKCPNKESETIKIKPLGFTEEVEVVLQFICKCNCQSHGIPASPKCHEGNGTFECGACR
  CNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRENTNEIYSGKFCECDNFNC
  DRSNGLICGGNGVCRCRVCECYPNYTGSACDCSLDTVPCVATNGQICNGRGICECGACKCTDPKFQGPTC
  ETCQTCLGVCAEHKECVQCRAFNKGEKKDTCAQECSHFNLTKVESREKLPQPVQVDPVTHCKEKDIDDCW
  FYFTYSVNSNGEAHVHVVETPDCPTGPDIIPIVAGVVAGIVLIGLALLLIWKLLMIIHDRREFAKFEKEK
  MNAKWDTGENPIYKSAVTTVVNPKYEGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017022
• ORF Size: 2394 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017022.2, NP_058718.2
• RefSeq Size: 3683 bp
• RefSeq ORF: 2397 bp
• Locus ID: 24511
• UniProt ID: P49134
• Cytogenetics: 19q12
• MW: 88.3 kDa
• Gene Summary: beta subunit of integrin receptor which binds collagen [RGD, Feb 2006]