C6 (NM_176074) Rat Tagged ORF Clone

CAT#: RR204161

  • TrueORF®

C6 (Myc-DDK-tagged ORF) - Rat complement component 6 (C6), (10 ug)

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro


  "NM_176074" in other vectors (3)

Reconstitution Protocol

USD 941.00

6 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 650.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

USD 198.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol C6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR204161 representing NM_176074
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCAGACATCTCACCTTGTGTTTCATTTTGCTGATCATACTGATTGACAAGAGTGAAGCCTGTTTCT
GTGACCACTACCCATGGACTCACTGGTCCAGCTGTTCTAAGTCCTGCAATTCTGGAACCCAGAGCAGACA
GAGACAAATCGTAGTGAACGATTACTATCGGGATAACTCATGCGATCAGCTCTGTACCAAGCAGGAGACC
AGACAGTGCAACGTGGAGACATGTCCCATCAACTGTGTCCTAGGAGACTATGGAACATGGTCAGACTGTG
ACCCTTGTATTAGAAAACAGGTTAAAGTTAGATCTGTTCTGCGCCCAAGTCAGTTTGGGGGACAACCATG
CACAGAGCCCCTGGTGACCTTTCAACCATGTGTCCCATCTGAGCTCTGCAAAATTGAAGAGACTGATTGC
AAGAATAAATTCCTCTGTGACAGTGGGCGCTGTATTCCCAGCAAGTTAAAATGCAATGGAGAAAATGACT
GTGGAGACAATTCAGACGAAAGGAACTGTGGGAGGACAAAGCCAGTATGCTCACGGACATACACTCCTAT
CCCCAGTGTGCAGCTGATGGGCGCTGGGTTTCATTTTCTGGCAGGAGAGCCCAGAGGAGATGTCCCTGAT
AACTCCTTCACTGGAGGAATATGTAAATCCGTCAGGAGCAGCCGAACGAGTAATCCACACCGAGTTCCAG
CCAATCTGGAAAATGTCAACTTTGAGGTACAAACTATAGAAGATGACTTGAAAACAGATTTCTACAAGGA
TTTAGCCACTATTGGAAAAAATAAAAATGAAGACCGTTCATTGTCTGGTGAGAAGAAAGACTCTTTCTAC
GTACCAATTTTTTATTCCTCAAAGAAAAGTGAAAATTTCCAACGTAACTCAGGCTTCAAAAACGCCATTG
AAGCCTCCCACAAGAAGGATTCGAGCTTTGTTAGGATCCATAAAGTGATAAAAGTCTTAAACTTCACAAT
GAAAACGACAGACCTGCAGCTCTCAGACGTCTTCCTAAAAGCCCTCATCCACCTGCCTTTAGAATACAAC
TTTGCTTTGTACAGCCGGATATTTGATGACTTTGGGACCCACTATTTCACCTCAGGCTCCCTTGGGGGCA
AGTATGACCTTCTCTACCAATTCAGCCGCCAGGAGCTACAGAACTCAGGTTTAACAGAAGAAGAAACTCG
AAACTGTGTCCGGTATGAAACAAAGAAACGTTTCTTATTTTTTACGAAAACATACAAGGAAGACCGGTGT
ACCACAAATAGGCTGTCTGAAAAGTACAAAGGTTCCTTTTTACAGGGATCGGAGAAATCTATATCCCTGG
TCCAGGGCGGGAGGAGTCAGCAGGCAGCAGCCTTGGCTTGGGAGAAGGGCAGCTCTGGTCCAGAGGCGAA
TGTCTTCTCTGAGTGGTTAGAGTCGGTGAAGGAAAACCCTGCTGTGGTTGATTATGAGCTTGCTCCGATC
ATCGACTTGGTCAGAAACATCCCATGTGCAGTGACAAAACGGAACAACCTCAGGAAAGCCCTTCAAGAAT
ATGCAGCCAAGTTTGACCCTTGCCAATGTGCTCCATGTCCTAATAATGGCCGCCCCAGGCTCTCAGGCAC
AGAATGTTTGTGTGTGTGCCAGAGTGGTACCTACGGTGAGAACTGTGAAAAACGGTCCCCAGATTATAAA
TCGAATGCAGTGGATGGGAACTGGGGCTGCTGGTCTTCCTGGAGCGCATGCAATGCTGCTTATAGGAGGT
CAAGGAGCCGGGAGTGTAATAACCCTGAGCCACAGCGAGGAGGGCAGCGCTGTGAGGGCAAGCATTGGCA
AGAAGAAGACTGTACATTCTCAATAATGGAAAAAGTTGGACAACCGTGCATCAGTGATGATGAAGAAATA
AAAGAAGTAGACCTTGCTGAGCCAGAAGCAGATTCAGGGTGTCCTCAGCCACCTCTCCCAGAAAATGCAT
TTGTCTGGAATGAAAAGAAACTGTACTCAGTCGGGGAGGAAGTTGAAATTTCATGTCTCACTGGATTCAA
AGCTGTGGGATACCAGTACTTCAGATGCTTACCAGACAGAACCTGGAGGCAAGGGGATGTCGAATGCCAA
CGGACCGAGTGCCTCAAACCAGTCGTTCAGGATGTCCTGACCATCTCCCCATTTCAGAGTGTGTACAAGA
TTGGGGAATCCATTGAGCTGACCTGTCCCAGAGGCTTTGTTGTTGCTGGCCCATCGAGGTATACATGCAA
GGGAGACTCCTGGACACCTCCCATTCCAAATTCACTGAGCTGTGAAAAAGATATTCTGACAAAGTCAAAG
GGCCTTTGTCAACCAGGACAAAAGCAATCAGGATCCGAGTGTGTTTGTATGTCCCCAGAAGAAGACTGTA
GCAGTTATTCGGAAGATCTCTGTATATTTGATGAGGGATCCAGTCAGTACTTCACTTCATCTGCTTGCAA
ATTTTTGGCTGAAAAATGTTTAAACAGCAACCAGTTCCACTTTGTCCATGCTGGTTCCTGCCAAGAAGGC
CCACAGTTAGAATGGGGTCTTGAGAGGCTAAAACTCGCAATGAAGAGCACAAAGAGAGTGCCCTGTGGAT
ATGATACTTGCTATGACTGGGAAAAATGTTCAGCCCACACCTCCAACTGTGTCTGCCTATTGCCCCCACA
ATGCCCCAAGGATGAAAACCAACTCCACTGTGTCAAAATGGGATCATCAATGCGTGGGAAAACAGTAAAC
ATCTGTACACTGGGAGCCGTGAGGTGTGCAAACAGGAAGGTGGAAATACTGAATCCTGGGAGGTGCTTGG
AT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR204161 representing NM_176074
Red=Cloning site Green=Tags(s)

MTRHLTLCFILLIILIDKSEACFCDHYPWTHWSSCSKSCNSGTQSRQRQIVVNDYYRDNSCDQLCTKQET
RQCNVETCPINCVLGDYGTWSDCDPCIRKQVKVRSVLRPSQFGGQPCTEPLVTFQPCVPSELCKIEETDC
KNKFLCDSGRCIPSKLKCNGENDCGDNSDERNCGRTKPVCSRTYTPIPSVQLMGAGFHFLAGEPRGDVPD
NSFTGGICKSVRSSRTSNPHRVPANLENVNFEVQTIEDDLKTDFYKDLATIGKNKNEDRSLSGEKKDSFY
VPIFYSSKKSENFQRNSGFKNAIEASHKKDSSFVRIHKVIKVLNFTMKTTDLQLSDVFLKALIHLPLEYN
FALYSRIFDDFGTHYFTSGSLGGKYDLLYQFSRQELQNSGLTEEETRNCVRYETKKRFLFFTKTYKEDRC
TTNRLSEKYKGSFLQGSEKSISLVQGGRSQQAAALAWEKGSSGPEANVFSEWLESVKENPAVVDYELAPI
IDLVRNIPCAVTKRNNLRKALQEYAAKFDPCQCAPCPNNGRPRLSGTECLCVCQSGTYGENCEKRSPDYK
SNAVDGNWGCWSSWSACNAAYRRSRSRECNNPEPQRGGQRCEGKHWQEEDCTFSIMEKVGQPCISDDEEI
KEVDLAEPEADSGCPQPPLPENAFVWNEKKLYSVGEEVEISCLTGFKAVGYQYFRCLPDRTWRQGDVECQ
RTECLKPVVQDVLTISPFQSVYKIGESIELTCPRGFVVAGPSRYTCKGDSWTPPIPNSLSCEKDILTKSK
GLCQPGQKQSGSECVCMSPEEDCSSYSEDLCIFDEGSSQYFTSSACKFLAEKCLNSNQFHFVHAGSCQEG
PQLEWGLERLKLAMKSTKRVPCGYDTCYDWEKCSAHTSNCVCLLPPQCPKDENQLHCVKMGSSMRGKTVN
ICTLGAVRCANRKVEILNPGRCLD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_176074
ORF Size 2802 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_176074.3, NP_788263.1
RefSeq Size 3036 bp
RefSeq ORF 2805 bp
Locus ID 24237
UniProt ID Q811M5
Cytogenetics 2q16
MW 105.1 kDa
Gene Summary component of the complement cascade for immune response; may be a component of the membrane attack complex [RGD, Feb 2006]

Other Versions

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.