Ar (NM_012502) Rat Tagged ORF Clone

CAT#: RR203819

Ar (Myc-DDK-tagged ORF) - Rat androgen receptor (Ar), (10 ug)

Product Images

Circular map for RR203819

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ar
• Synonyms: Andr; Tfm
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203819 representing NM_012502
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTGCAGTTAGGGCTGGGAAGGGTCTACCCACGGCCCCCGTCCAAGACCTATCGAGGAGCGTTCC
  AGAATCTGTTCCAGAGCGTGCGCGAAGCGATCCAGAACCCGGGCCCCAGGCACCCTGAGGCCGCTAGCAT
  AGCACCTCCCGGTGCCTGTTTACAGCAGCGGCAGGAGACTAGCCCCCGGCGGCGGCGGCGGCAGCAGCAC
  CCTGAGGATGGCTCTCCTCAAGCCCACATCAGAGGCACCACAGGCTACCTGGCCCTGGAGGAGGAACAGC
  AGCCTTCACAGCAGCAGTCAGCCTCCGAGGGCCACCCTGAGAGCGGCTGCCTCCCGGAGCCTGGAGCTGC
  CACGGCTCCTGGCAAGGGGCTGCCGCAGCAGCCACCAGCTCCTCCAGATCAGGATGACTCAGCTGCCCCA
  TCCACGTTGTCCCTACTGGGCCCCACTTTCCCAGGCTTAAGCAGCTGCTCCGCAGACATTAAAGACATCC
  TGAGCGAGGCCGGCACCATGCAACTTCTTCAGCAGCAGCAGCAACAGCAACAGCAGCAGCAGCAGCAGCA
  GCAGCAGCAGCAGCAACAGCAGCAGGAGGTAATATCCGAAGGCAGCAGCAGCGTGAGAGCAAGGGAGGCC
  ACTGGGGCTCCCTCTTCCTCCAAGGATAGTTACCTAGGGGGCAATTCGACCATATCTGACAGTGCCAAGG
  AGTTGTGTAAAGCAGTGTCTGTGTCCATGGGGTTGGGTGTGGAAGCACTGGAACATCTGAGTCCAGGGGA
  GCAGCTTCGGGGCGACTGCATGTACGCGTCGCTCCTGGGAGGTCCACCCGCCGTGCGTCCCACTCCTTGT
  GCGCCTCTGGCCGAATGCAAAGGTCTTTCCCTGGACGAAGGCCCGGGCAAAGGCACTGAAGAGACTGCTG
  AGTATTCCTCTTTCAAGGGAGGTTACGCCAAAGGGTTGGAAGGTGAGAGTCTGGGCTGCTCTGGCAGCAG
  TGAAGCAGGTAGCTCTGGGACACTTGAGATCCCGTCCTCACTGTCTCTGTATAAGTCTGGAGCAGTAGAC
  GAGGCAGCAGCATACCAGAATCGCGACTACTACAACTTTCCGCTCGCTCTGTCCGGGCCGCCGCACCCCC
  CGCCCCCTACCCATCCACACGCCCGCATCAAGCTGGAGAACCCGTCGGACTACGGCAGCGCCTGGGCTGC
  GGCGGCAGCGCAATGCCGCTATGGGGACTTGGCTAGCCTACATGGAGGGAGTGTAGCCGGACCCAGCACT
  GGATCGCCCCCAGCCACCGCCTCTTCTTCCTGGCATACTCTCTTCACAGCTGAAGAAGGCCAATTATATG
  GGCCAGGAGGCGGGGGCGGCAGCAGTAGCCCAAGCGATGCTGGGCCTGTAGCCCCCTATGGCTACACTCG
  GCCCCCTCAGGGGCTGGCAAGCCAGGAGGGTGACTTCTCTGCCTCTGAAGTGTGGTATCCTGGTGGAGTT
  GTGAACAGAGTCCCCTATCCCAGTCCCAGTTGTGTTAAAAGTGAAATGGGACCTTGGATGGAGAACTACT
  CCGGACCTTATGGGGACATGCGTTTGGACAGTACCAGGGACCACGTTTTACCCATCGACTATTACTTCCC
  ACCCCAGAAGACCTGCCTGATCTGTGGAGATGAAGCTTCTGGTTGTCACTACGGAGCTCTCACTTGTGGC
  AGCTGCAAGGTCTTCTTCAAAAGAGCTGCGGAAGGGAAACAGAAGTATCTATGTGCCAGCAGAAATGATT
  GCACCATTGATAAATTTCGGAGGAAAAATTGTCCATCGTGTCGTCTCCGGAAATGTTATGAAGCAGGGAT
  GACTCTGGGAGCTCGTAAGCTGAAGAAACTTGGAAATCTCAAACTACAGGAAGAAGGAGAAAACTCCAGT
  GCTGGTAGCCCCACTGAGGACCCATCCCAGAAGATGACTGTATCACACATTGAAGGCTATGAATGTCAAC
  CTATCTTTCTTAATGTCCTGGAAGCCATTGAGCCAGGAGTGGTGTGTGCCGGACATGACAACAACCAGCC
  TGATTCCTTTGCTGCCTTGTTATCTAGTCTCAACGAGCTTGGCGAGAGACAGCTTGTACATGTGGTCAAG
  TGGGCCAAGGCCTTGCCTGGCTTCCGCAACTTGCATGTGGATGACCAGATGGCAGTCATTCAGTATTCCT
  GGATGGGACTGATGGTATTTGCCATGGGTTGGCGGTCCTTCACTAATGTCAACTCTAGGATGCTCTACTT
  TGCACCTGACCTGGTTTTCAATGAGTATCGCATGCACAAGTCTCGAATGTACAGCCAGTGCGTGAGGATG
  AGGCACCTTTCTCAAGAGTTTGGATGGCTCCAGATAACCCCCCAGGAATTCCTGTGCATGAAAGCACTGC
  TACTCTTCAGCATTATTCCAGTGGATGGGCTGAAAAATCAAAAATTCTTTGATGAACTTCGAATGAACTA
  CATCAAGGAACTTGATCGCATCATTGCATGCAAAAGAAAAAATCCCACATCCTGCTCAAGGCGCTTCTAC
  CAGCTCACCAAGCTCCTGGATTCTGTGCAGCCTATTGCAAGAGAGCTGCATCAATTCACTTTTGACCTGC
  TAATCAAGTCCCATATGGTGAGCGTGGACTTTCCTGAAATGATGGCAGAGATCATCTCTGTGCAAGTGCC
  CAAGATCCTTTCTGGGAAAGTCAAGCCCATCTATTTCCACACACAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203819 representing NM_012502
  Red=Cloning site Green=Tags(s)
  
  MEVQLGLGRVYPRPPSKTYRGAFQNLFQSVREAIQNPGPRHPEAASIAPPGACLQQRQETSPRRRRRQQH
  PEDGSPQAHIRGTTGYLALEEEQQPSQQQSASEGHPESGCLPEPGAATAPGKGLPQQPPAPPDQDDSAAP
  STLSLLGPTFPGLSSCSADIKDILSEAGTMQLLQQQQQQQQQQQQQQQQQQQQQQEVISEGSSSVRAREA
  TGAPSSSKDSYLGGNSTISDSAKELCKAVSVSMGLGVEALEHLSPGEQLRGDCMYASLLGGPPAVRPTPC
  APLAECKGLSLDEGPGKGTEETAEYSSFKGGYAKGLEGESLGCSGSSEAGSSGTLEIPSSLSLYKSGAVD
  EAAAYQNRDYYNFPLALSGPPHPPPPTHPHARIKLENPSDYGSAWAAAAAQCRYGDLASLHGGSVAGPST
  GSPPATASSSWHTLFTAEEGQLYGPGGGGGSSSPSDAGPVAPYGYTRPPQGLASQEGDFSASEVWYPGGV
  VNRVPYPSPSCVKSEMGPWMENYSGPYGDMRLDSTRDHVLPIDYYFPPQKTCLICGDEASGCHYGALTCG
  SCKVFFKRAAEGKQKYLCASRNDCTIDKFRRKNCPSCRLRKCYEAGMTLGARKLKKLGNLKLQEEGENSS
  AGSPTEDPSQKMTVSHIEGYECQPIFLNVLEAIEPGVVCAGHDNNQPDSFAALLSSLNELGERQLVHVVK
  WAKALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNVNSRMLYFAPDLVFNEYRMHKSRMYSQCVRM
  RHLSQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYIKELDRIIACKRKNPTSCSRRFY
  QLTKLLDSVQPIARELHQFTFDLLIKSHMVSVDFPEMMAEIISVQVPKILSGKVKPIYFHTQ
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012502
• ORF Size: 2706 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012502.1, NP_036634.1
• RefSeq Size: 4137 bp
• RefSeq ORF: 2709 bp
• Locus ID: 24208
• UniProt ID: P15207
• Cytogenetics: Xq22
• MW: 98.2 kDa
• Gene Summary: binds androgens and activates transcription; required for male sexual development [RGD, Feb 2006]