Nme7 (NM_138532) Rat Tagged ORF Clone

CAT#: RR203382

Nme7 (Myc-DDK-tagged ORF) - Rat non-metastatic cells 7, protein expressed in (nucleoside-diphosphate kinase) (Nme7), (10 ug)

Product Images

Circular map for RR203382

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nme7
• Synonyms: Nm23-r7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203382 representing NM_138532
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGCTGGTCAGCAGGGAAGAAGTTGCGGTTTGATTTCTCCGTATCTTGCACCCAAGAATCAGAGTG
  AGAGGTTTGCTTTCATTGCAGAGTGGTATGACCCAAATGCTTCACTGCTCCGACGCTATGAGCTTCTGTA
  CTACCCGGTAGATGGATCTGTTGAAATGCATGATGTCAAGAATCGTCGCACCTTCTTAAAGCGGACCAAG
  TATGAGGACCTGCGCGTGGAAGATTTATTTATAGGCAACAAAGTCAATGTGTTTTCTCGACAGCTGGTGC
  TGATTGACTATGGGGACCAGTACACAGCCCGCCAGCTGGGCAGCAGGAAAGAGAAAACTTTAGCCCTCAT
  CAAACCGGATGCAGTATCGAAGGCTGGAGAAATCATTGAGATGATAAACAAAAGTGGGTTCACTATAACC
  AAACTCCGAATGATGACGCTGTCAAGGAAAGAAGCAGCAGACTTTCATGTAGACCACCACTCAAGGCCTT
  TTTATAACGAACTGATCCAGTTTATCACAAGTGGGCCTGTTATTGCCATGGAGATCTTGAGAGATGATGC
  CATATGTGAGTGGAAAAGGTTGCTTGGACCCGCAAACTCTGGGATAGCGCGTTCAGAAGCCCCCGGAAGC
  GTCCGAGCCCTCTTTGGGACAGATGGCATAAGAAATGCGGCTCACGGCTCTGATACTTTTGAGTCCGCTG
  CCAGAGAAATGGAATTATTTTTCCCCTCCAGTGGAGGCTGTGGCCCAACAAACACTGCTAAATTTACCAA
  TTGTACCTGCTGCATTATCAAGCCCCATGCTATCAGTGAAGGAATGTTGGGAAAGATTTTAATAGCTATT
  CGAGATGCATGCTTTGAGATCTCAGCAATACAGATGTTCAATATGGATCGAGCTAATGTTGAAGAATTCT
  ATGAAGTCTATAAAGGTGTACTGTCCGATTATAATGACATGGTGACAGAGCTGTACTCCGGCCCTTGCGT
  AGCAATAGAGATCCAGCAGAGCAATCCTACGAAGACGTTCCGAGAATTCTGTGGACCTTCCGATCCTGAA
  ATCGCCAGGCATTTACGACCTGAGACCCTCAGGGCAAATTTTGGTAAAACCAAGGTTCAAAATGCTGTTC
  ATTGTACAGATCTGCCAGAGGATGGGCTCCTGGAGGTCCAGTATTTCTTCAAGATCTTGGATAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203382 representing NM_138532
  Red=Cloning site Green=Tags(s)
  
  MKAGQQGRSCGLISPYLAPKNQSERFAFIAEWYDPNASLLRRYELLYYPVDGSVEMHDVKNRRTFLKRTK
  YEDLRVEDLFIGNKVNVFSRQLVLIDYGDQYTARQLGSRKEKTLALIKPDAVSKAGEIIEMINKSGFTIT
  KLRMMTLSRKEAADFHVDHHSRPFYNELIQFITSGPVIAMEILRDDAICEWKRLLGPANSGIARSEAPGS
  VRALFGTDGIRNAAHGSDTFESAAREMELFFPSSGGCGPTNTAKFTNCTCCIIKPHAISEGMLGKILIAI
  RDACFEISAIQMFNMDRANVEEFYEVYKGVLSDYNDMVTELYSGPCVAIEIQQSNPTKTFREFCGPSDPE
  IARHLRPETLRANFGKTKVQNAVHCTDLPEDGLLEVQYFFKILDN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138532
• ORF Size: 1185 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138532.1, NP_612541.1
• RefSeq Size: 1659 bp
• RefSeq ORF: 1188 bp
• Locus ID: 171566
• UniProt ID: Q9QXL7
• Cytogenetics: 13q23
• MW: 44.5 kDa
• Gene Summary: nucleoside-diphosphate kinase important for purine and pyrimidine biosynthesis [RGD, Feb 2006]