Ephb2 (NM_001127319) Rat Tagged ORF Clone

CAT#: RR203198

Ephb2 (Myc-DDK-tagged ORF) - Rat Eph receptor B2 (Ephb2), (10 ug)

Product Images

Circular map for RR203198

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ephb2
• Synonyms: RGD1564232
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203198 representing NM_001127319
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGTGCGCAGGCTGGGGGCCGCGCTGCTGCTGCTGCCGCTGCTAGCCGCCGTGGAAGAAACACTGA
  TGGACTCTACTACAGCAACGGCTGAGCTGGGCTGGATGGTACATCCCCCATCAGGGTGGGAAGAGGTGAG
  CGGCTACGATGAGAACATGAACACTATCCGTACCTACCAGGTGTGCAATGTCTTTGAGTCAAGCCAGAAC
  AACTGGCTACGGACCAAATTCATCCGGCGCCGCGGCGCCCACCGCATCCACGTGGAGATGAAGTTCTCGG
  TGCGTGACTGTAGCAGCATTCCCAGCGTGCCCGGCTCCTGCAAGGAGACCTTCAACCTCTACTACTATGA
  GGCTGATTTTGACTTAGCCACCAAGACCTTTCCCAACTGGATGGAGAATCCGTGGGTGAAGGTGGACACC
  ATTGCGGCTGATGAGAGCTTTTCCCAGGTGGATCTGGGTGGCCGCGTCATGAAAATCAACACTGAGGTGC
  GAAGCTTCGGTCCCGTGTCCCGCAATGGTTTCTACCTGGCCTTCCAGGACTACGGCGGCTGTATGTCCCT
  CATTGCGGTGCGCGTCTTCTACCGGAAGTGCCCCCGAGTCATCCAGAATGGTGCCATCTTCCAGGAGACA
  CTGTCGGGGGCTGAGAGCACCTCACTGGTAGCTGCTCGGGGCAGCTGCATCGCCAACGCTGAAGAAGTGG
  ATGTACCCATCAAACTCTACTGTAACGGGGACGGCGAATGGCTGGTGCCCATCGGTCGCTGCATGTGCAA
  GGCGGGCTTCGAGGCTGTGGAGAACGGTACCGTCTGCCGAGGTTGTCCATCAGGAACCTTCAAGGCCAAC
  CAAGGGGATGAAGCCTGTACCCACTGTCCCATCAACAGCCGTACCACCTCCGAGGGTGCCACCAACTGTG
  TGTGCCGTAACGGCTACTACAGGGCTGACCTGGACCCCTTAGACATGCCTTGCACAACCATCCCCTCTGC
  GCCCCAGGCTGTGATCTCCAGTGTCAACGAGACGTCCCTCGTGCTAGAGTGGACCCCGCCACGAGACTCG
  GGGGGCCGTGAGGACCTTGTTTATAACATCATCTGCAAGAGCTGTGGCTCAGGCCGGGGTGCCTGCACGC
  GCTGTGGAGACAATGTGCAGTACGCACCCCGCCAGCTAGGCCTGACCGAGCCACGCATCTACATCAGTGA
  CCTGCTGGCACACACACAGTACACCTTCGAGATCCAGGCCGTGAATGGTGTCACCGACCAGAGTCCCTTC
  TCACCTCAGTTCGCCTCTGTGAACATCACCACCAACCAAGCAGCACCGTCGGCCGTGTCCATCATGCACC
  AGGTGAGCCGCACCGTGGACAGCATCACCCTGTCGTGGTCCCAGCCAGACCAGCCCAACGGTGTGATCCT
  GGACTATGAACTACAGTACTATGAGAAGGAGCTCAGTGAGTACAACGCCACAGCCATAAAAAGCCCCACC
  AACACAGTCACTGTGCAGGGCCTCAAAGCCGGCACCATCTATGTCTTCCAGGTGCGGGCACGCACCGTCG
  CAGGCTATGGGCGTTACAGTGGCAAGATGTACTTCCAAACCATGACAGAAGCCGAGTACCAGACCAGCAT
  CAAGGAAAAGCTGCCCCTCATCGTCGGCTCCTCCGCTGCCGGCGTAGTCTTCGTCATCGCCGTGGTCGTC
  ATTGCCATCGTATGTAACAGACGGGGGTTTGAGCGTGCCGACTCAGAGTACACGGACAAGCTGCAGCACT
  ACACCAGCGGCCACATGACCCCAGGCATGAAGATCTATATTGACCCTTTCACCTATGAAGATCCTAATGA
  GGCAGTGCGGGAGTTTGCCAAGGAAATCGACATCTCCTGTGTCAAGATTGAGCAGGTGATTGGAGCAGGG
  GAATTTGGTGAGGTCTGCAGTGGCCACTTGAAGCTGCCAGGGAAGAGAGAGATCTTTGTAGCCATCAAGA
  CCCTCAAGTCAGGATACACAGAGAAACAGCGCCGGGACTTCCTGAGTGAGGCATCCATCATGGGCCAGTT
  CGACCACCCCAATGTCATCCACCTGGAAGGGGTTGTCACCAAGAGCACACCTGTCATGATCATCACTGAA
  TTCATGGAGAACGGTTCCCTGGACTCCTTCCTCCGGCAAAACGATGGGCAGTTCACGGTCATCCAACTGG
  TGGGCATGCTGAGGGGCATCGCAGCTGGCATGAAGTACCTGGCGGACATGAACTATGTGCACCGGGACCT
  TGCTGCACGCAACATCCTTGTCAATAGCAACCTGGTGTGCAAGGTGTCTGACTTCGGGCTCTCACGCTTC
  CTGGAGGACGACACATCTGACCCCACCTACACCAGCGCTCTGGGTGGGAAGATCCCCATCCGTTGGACAG
  CACCGGAAGCCATCCAGTACCGGAAATTCACCTCAGCCAGTGATGTGTGGAGCTATGGCATTGTCATGTG
  GGAGGTGATGTCCTACGGGGAGCGACCCTACTGGGACATGACCAACCAAGACGTAATCAATGCCATCGAG
  CAGGACTACCGGCTACCTCCGCCCATGGACTGCCCCAGCGCGCTGCACCAGCTCATGCTGGATTGCTGGC
  AGAAGGACCGCAACCACCGGCCCAAGTTCGGCCAGATCGTCAACACGCTGGACAAGATGATCCGAAACCC
  CAACAGCCTCAAAGCTATGGCGCCCCTGTCCTCTGGTATCAACCTGCCACTACTGGACCGCACGATACCC
  GACTACACCAGCTTTAACACAGTGGATGAGTGGCTGGAGGCCATCAAGATGGGCCAGTACAAGGAGAGCT
  TTACCAACGCCGGCTTCACCTCTTTTGACGTCGTATCTCAGATGATGATGGAGGACATTCTCCGGGTTGG
  GGTCACTCTAGCTGGCCACCAGAAAAAAATCCTGAACAGTATCCAGGTGATGCGGGCCCAGATGAACCAG
  ATCCAGTCGGTAGAGGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203198 representing NM_001127319
  Red=Cloning site Green=Tags(s)
  
  MAVRRLGAALLLLPLLAAVEETLMDSTTATAELGWMVHPPSGWEEVSGYDENMNTIRTYQVCNVFESSQN
  NWLRTKFIRRRGAHRIHVEMKFSVRDCSSIPSVPGSCKETFNLYYYEADFDLATKTFPNWMENPWVKVDT
  IAADESFSQVDLGGRVMKINTEVRSFGPVSRNGFYLAFQDYGGCMSLIAVRVFYRKCPRVIQNGAIFQET
  LSGAESTSLVAARGSCIANAEEVDVPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCRGCPSGTFKAN
  QGDEACTHCPINSRTTSEGATNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLVLEWTPPRDS
  GGREDLVYNIICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPF
  SPQFASVNITTNQAAPSAVSIMHQVSRTVDSITLSWSQPDQPNGVILDYELQYYEKELSEYNATAIKSPT
  NTVTVQGLKAGTIYVFQVRARTVAGYGRYSGKMYFQTMTEAEYQTSIKEKLPLIVGSSAAGVVFVIAVVV
  IAIVCNRRGFERADSEYTDKLQHYTSGHMTPGMKIYIDPFTYEDPNEAVREFAKEIDISCVKIEQVIGAG
  EFGEVCSGHLKLPGKREIFVAIKTLKSGYTEKQRRDFLSEASIMGQFDHPNVIHLEGVVTKSTPVMIITE
  FMENGSLDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLADMNYVHRDLAARNILVNSNLVCKVSDFGLSRF
  LEDDTSDPTYTSALGGKIPIRWTAPEAIQYRKFTSASDVWSYGIVMWEVMSYGERPYWDMTNQDVINAIE
  QDYRLPPPMDCPSALHQLMLDCWQKDRNHRPKFGQIVNTLDKMIRNPNSLKAMAPLSSGINLPLLDRTIP
  DYTSFNTVDEWLEAIKMGQYKESFTNAGFTSFDVVSQMMMEDILRVGVTLAGHQKKILNSIQVMRAQMNQ
  IQSVEV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001127319
• ORF Size: 2958 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127319.1, NP_001120791.1
• RefSeq Size: 2970 bp
• RefSeq ORF: 2961 bp
• Locus ID: 313633
• Cytogenetics: 5q36
• MW: 109.9 kDa