Fbp1 (NM_012558) Rat Tagged ORF Clone

CAT#: RR203095

Fbp1 (Myc-DDK-tagged ORF) - Rat fructose-1,6- biphosphatase 1 (Fbp1), (10 ug)

Product Images

Circular map for RR203095

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fbp1
• Synonyms: Fdp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203095 representing NM_012558
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGACCATGCGCCCTTCGAAACGGATATCAGCACCCTGACCCGCTTCGTCCTGGAGGAGGGCAGGA
  AGGCTGGGGGCACGGGCGAGATGACCCAGCTGCTGAATTCGCTCTGCACCGCGATCAAAGCCATCTCGTC
  AGCGGTGCGCCAGGCCGGCATCGCTCAGCTCTATGGCATCGCTGGCTCAACCAATGTGACTGGGGATCAA
  GTGAAGAAGCTGGACATACTTTCCAATGACCTGGTGATCAATATGCTGAAGTCGTCCTATGCTACCTGTG
  TCCTTGTGTCGGAAGAAGATACACACGCCATCATAATAGAGCCCGAGAAGAGGGGCAAATATGTTGTCTG
  TTTTGATCCCCTCGATGGCTCATCCAACATCGACTGCCTCGCGTCCATCGGAACCATCTTTGGCATTTAC
  AGAAAGACATCTGCTAATGAGCCTTCGGAGAAAGATGCTCTGCAGCCAGGCAGGAACCTGGTGGCAGCAG
  GCTATGCACTCTATGGCAGCGCCACCATGTTGGTCCTGGCCATGAATTGTGGCGTCAACTGCTTCATGCT
  GGACCCGTCCATTGGAGAATTCATTTTGGTGGACAGGGATGTGAAGATCAAGAAGAAAGGTAACATCTAC
  AGCATTAATGAGGGCTATGCTAAGGACTTTGACCCTGCCATCAATGAGTATATCCAGAGGAAAAAGTTCC
  CTCCGGATAATTCAGCTCCCTATGGTGCCCGGTATGTGGGGTCCATGGTGGCTGATGTTCACCGCACTCT
  GGTGTATGGAGGGATCTTCTTATACCCTGCCAACAAGAAAAATCCATCTGGAAAGCTGCGGCTGCTGTAC
  GAGTGCAATCCCATCGCTTACGTCATGGAGAAGGCCGGTGGACTTGCCACGACGGGGAATGAAGACATAT
  TAGACATCGTCCCCACTGAGATCCACCAGAAGGCACCAGTTATCATGGGGTCCACCGAAGACGTGCAGGA
  GTTCCTGGAGATCTACAATAAGGACAAAGCCAAATCAAGGCCGTCCCTGCCCCTGCCCCAGAGCAGAGCT
  CGAGAGTCACCGGTCCATTCTATCTGTGACGAACTTTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203095 representing NM_012558
  Red=Cloning site Green=Tags(s)
  
  MVDHAPFETDISTLTRFVLEEGRKAGGTGEMTQLLNSLCTAIKAISSAVRQAGIAQLYGIAGSTNVTGDQ
  VKKLDILSNDLVINMLKSSYATCVLVSEEDTHAIIIEPEKRGKYVVCFDPLDGSSNIDCLASIGTIFGIY
  RKTSANEPSEKDALQPGRNLVAAGYALYGSATMLVLAMNCGVNCFMLDPSIGEFILVDRDVKIKKKGNIY
  SINEGYAKDFDPAINEYIQRKKFPPDNSAPYGARYVGSMVADVHRTLVYGGIFLYPANKKNPSGKLRLLY
  ECNPIAYVMEKAGGLATTGNEDILDIVPTEIHQKAPVIMGSTEDVQEFLEIYNKDKAKSRPSLPLPQSRA
  RESPVHSICDELF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012558
• ORF Size: 1089 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012558.3, NP_036690.2
• RefSeq Size: 1474 bp
• RefSeq ORF: 1092 bp
• Locus ID: 24362
• UniProt ID: P19112
• Cytogenetics: 17p14
• MW: 39.6 kDa
• Gene Summary: catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate in gluconeogenesis [RGD, Feb 2006]