Pfas (NM_001105791) Rat Tagged ORF Clone

CAT#: RR203065

Pfas (Myc-DDK-tagged ORF) - Rat phosphoribosylformylglycinamidine synthase (Pfas), (10 ug)

Product Images

Circular map for RR203065

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pfas
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203065 representing NM_001105791
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTTGCTCGGGAGTCCTGGCTGGTCCCTGGCTCCAATGATTTGTTGCTGGAGGTGGGACNNGCTGA
  ATTTCTCTACTCCAGCATCGACCAACATTGTTTCTGTGTGCCAGGCTGCTGGGCTGAGAGCTGTGGATCG
  GGTAGAGACCACCCGGCGCTACCGGCTTTCGTTTGCCAAGCAGCCTACAGCTGAAATGGAAGCCATTTCT
  CTGGCTGCCTTGCATGACCGGATGACAGAGCAGCACTACCCTGACCCCATTCAGAGCTTCTCTCCTCAGA
  GCATCCCAGCCCCACTCAAGGACTCCATCAACATCCTAGCCGAGGGTCGGCCTGCCCTGGAGAAGGCCAA
  CCAGGAGCTAGGTTTGGCTCTTGACTCCTGGGACCTCGATTTCTACACCAAGCGCTTTCAAGAGCTGCAG
  CGAAACCCCAGTACAGTGGAGGTCTTTGACTTGGCTCAGTCCAATAGTGAGCACAGCCGACATTGGTTCT
  TCAAGGGCCAGCTCCATGTGGACGGGAAGAAGCTAGCACGTTCCCTCTTTGAATCCATCATGAGCACTCA
  GTCATCCTCCAACCCTAACAATGTCCTCAAGTTCTGTGACAACAGCAGTGCAATCCAAGGGAAGGAAGTC
  CGATTCCTGCGGCCTGAGGACTCTACACGACCAAGCTGCTTCCGGCAACAGCGGGGGCTTAGACATGTTG
  TCTTCACAGCAGAGACACATAACTTCCCCACCGGAGTGGCGCCCTTCAGCGGTGCAACCACAGGCACAGG
  TGGCCGCATCAGAGATGTCCAGTGTACAGGCCGTGGAGCCCACGTGGTGGCTGGCACTGCTGGCTATTGC
  TTTGGGAACCTGCATATCCCAGGTTATAATCTGCCCTGGGAGGATCCAAGCTTCCAGTATCCTGGGAACT
  TCGCCAGGCCTTTGGAAGTTGCTATTGAGGCCAGTAATGGAGCATCTGACTATGGCAACAAGTTTGGAGA
  ACCAGTGCTGGCTGGATTTGCCCGCTCTTTGGGTCTCCAGCTTCCAGATGGTCAGAGACGTGAGTGGATC
  AAGCCCATCATGTTCAGTGGGGGCATTGGGTCCATGGAAGCCAAGCATGTAGGCAAAGAACCCCCAGAGC
  CAGGCATGGAGGTTGTGAAGGTCGGGGGTCCCGTCTACAGGATTGGAGTTGGGGGTGGAGCTGCTTCGTC
  CGTGCAGGTTCAGGGAGACAACACCAGTGACCTGGACTTCGGAGCTGTGCAGCGGGGAGATCCGGAAATG
  GAGCAGAAGATGAATCGTGTGATCCGGGCTTGTGTGGAGGCCCCAGGAGGAAACCCCATATGCAGCCTCC
  ATGACCAGGGTGCTGGTGGCAATGGCAATGTCCTAAAGGAGCTGAGTGAGCCAGCGGGAGCTGTCATTTA
  CACCAGCCGCTTTCAGCTGGGTGATCCAACCTTGAATGCACTGGAAATCTGGGGGGCTGAGTACCAGGAG
  TCAAATGCACTTCTTCTGAGGCCCTCTGACCGGGACTTTCTGAGTCGCGCCAGTGCCCGGGAACGCTGCC
  CAGCTTGTTTTGTGGGCACAATCACTGGAGACAAGAGAATCGTGCTAGTGGACGACCGCGAATGCTTGAT
  GGGAAAAAGTGGCCAGGGAGATGCTCCCCCGACACCTCCAACTCCTGTGGACCTGGACCTTGACTGGGTT
  CTGGGAAAGATGCCTCAGAAGGAGTTCTTCCTCCAGAGAAAACCCCCTGTGCTGCAGCCTTTGGCCTTGC
  CCCCCGACCTGAGTGTGGGCCAGGCTCTAGAACGGGTTCTAAGGCTGCCTGCTGTGGCCAGCAAGCGCTA
  CCTCACTAACAAGGTGGATCGCTCTGTGGGTGGCCTTGTTGCCCAACAGCAGTGTGTCGGGCCCCTGCAG
  ACTCCTCTGGCTGATGTGGCTGTTGTAGCACTGAGTCACCAGGAGCTTGTAGGGGCCGCCACAGCCCTGG
  GAGAGCAGCCAGTCAAGAGCCTGCTGGACCCCAAGGCTGCTGCCAGGCTAGCCGTGTCTGAGGCCCTCAC
  CAACCTGGTATTCGCTCTGGTCACTGATCTCCGAGATGTGAAGTGCAGTGGGAACTGGATGTGGGCAGCC
  AAGCTCCCGGGTGAGGGTGCAGGGCTAGCCGATGCCTGTGAGGCTATGGTGACAGTGATGGCAGCACTGG
  GTGTGGCAGTAGATGGTGGCAAGGACTCCCTCAGCATGGCTGCCCGGGTCGGCACTGAGACCGTGCCAGC
  TCCTGGGTCACTGGTCATTTCAGCATATGCTGTCTGTCCAGACATCACAGCCACTGTGACCCCAGACCTC
  AAGCATCCTGGAGGAAAAGGTCATTTGCTCTATGTGCCTTTGAGCCCTGGGCAGCACCGGCTGGGAGGAA
  CGGCTCTGGCCCAATGTTTCTCTCAGCTCGGGGAACACCCTCCTGATCTAGACCTTCCCGAGAACCTCGT
  GCGTGCCTTTCATATCACCCAGGGGCTGCTGAAAGGTGAGTGGGGCCCTGATCGCCGTCTTTGCTCAGGC
  CATGATGTCAGCGATGGAGGGCTTGTAACCTGCCTTCTAGAGATGGCCTTTGCTGGGAATTGTGGGATAG
  AGGTAGATGTACCTGCCCCTGGGATCCATGCATTGCCTGTGCTGTTCGCAGAAGAGCCAGGTCTGGTGTT
  GGAAGTACAGGAGGCAGATGTAGCCGGAGTACTGCAGAGGTACCAGAGTGCTGGCCTACACTGTCTGGAA
  CTAGGCCACACTGGCGAGGCTGGACCCCAGGCCATGGTCCGAGTGTCAGTGAATGGGACTGTGGTTGTAG
  AGGAGCCTGTTGGGCAGTTGAGAGCCCTCTGGGAGGAAACAAGTTTCCAGCTGGACCTACTGCAGGCAGA
  GCCACGCTGTGTGACAGAGGAGAAACAGGGCCTGAAGGAACGGATAGGACCTAGCTACTGCCTGCCGCCT
  ACCTTTCCTGTGGCCTCTGTGCCCTGTAAACCTGGTGGTCCCATCCCTCGAGTTGCCATCTTGCGTGAAG
  AGGGCAGCAATGGAGACCGAGAGATGGCTGATGCTTTTCACTTGGCTGGCTTTGAGGTGTGGGATGTGAC
  CATGCAGGACCTCTGCTCTGGGGCCGTCAGGCTGGACACGTTCCGTGGTGTGGCCTTTGTGGGTGGCTTC
  AGCTACGCAGATGTCCTGGGCTCTGCCAAAGGTTGGGCAGCTGCAGTGACCTTCAATCCACAGGCCAGGG
  AAGAGCTGGGCCGCTTCCGCAGGCGGCCAGACACCTTCAGCCTGGGCGTGTGTAATGGCTGTCAGCTGCT
  GGCTCTTCTGGGCTGGGTGGGAAGTGATCCCAATGAAGACCAAGTTGAGCCGGACCACGACTCCCAGCCA
  GCACAGCCAGGCCTCCTGCTCCGTCACAATCTCTCTGGGCGGTTTGAGTCCCGATGGGCCACTGTACGTG
  TGGAGCCGGGGCCAGCCCTGATGCTTCGAGGGATGGAAGGCTCTGTGCTGCCTGTGTGGAGTGCTCATGG
  GGAAGGTTACATGGCATTTTCTTCTCCTGAACTTCAAGCCAAGATCAAGGCCAAGGGCTTGGTTCCTCTG
  CACTGGGCAGATGATGATGGGAACCCCACAGAACAGTACCCTTTGAACCCCAATGGATCTCCAGGGGGCA
  TAGCTGGCATCTGCTCCCCAGATGGTCGCCATCTGGCACTCATGCCACACCCTGAGCGGGCTGTGAGGCT
  TTGGCAGTGGGCATGGCGACCCTCTCCCTTTGATGGTCTTTCCACCTCCCCGTGGCTGCAGCTCTTCATC
  AATGCTCGGAACTGGACCCAGGAGGACAGCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203065 representing NM_001105791
  Red=Cloning site Green=Tags(s)
  
  MMLLGSPGWSLAPMICCWRWDXLNFSTPASTNIVSVCQAAGLRAVDRVETTRRYRLSFAKQPTAEMEAIS
  LAALHDRMTEQHYPDPIQSFSPQSIPAPLKDSINILAEGRPALEKANQELGLALDSWDLDFYTKRFQELQ
  RNPSTVEVFDLAQSNSEHSRHWFFKGQLHVDGKKLARSLFESIMSTQSSSNPNNVLKFCDNSSAIQGKEV
  RFLRPEDSTRPSCFRQQRGLRHVVFTAETHNFPTGVAPFSGATTGTGGRIRDVQCTGRGAHVVAGTAGYC
  FGNLHIPGYNLPWEDPSFQYPGNFARPLEVAIEASNGASDYGNKFGEPVLAGFARSLGLQLPDGQRREWI
  KPIMFSGGIGSMEAKHVGKEPPEPGMEVVKVGGPVYRIGVGGGAASSVQVQGDNTSDLDFGAVQRGDPEM
  EQKMNRVIRACVEAPGGNPICSLHDQGAGGNGNVLKELSEPAGAVIYTSRFQLGDPTLNALEIWGAEYQE
  SNALLLRPSDRDFLSRASARERCPACFVGTITGDKRIVLVDDRECLMGKSGQGDAPPTPPTPVDLDLDWV
  LGKMPQKEFFLQRKPPVLQPLALPPDLSVGQALERVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLQ
  TPLADVAVVALSHQELVGAATALGEQPVKSLLDPKAAARLAVSEALTNLVFALVTDLRDVKCSGNWMWAA
  KLPGEGAGLADACEAMVTVMAALGVAVDGGKDSLSMAARVGTETVPAPGSLVISAYAVCPDITATVTPDL
  KHPGGKGHLLYVPLSPGQHRLGGTALAQCFSQLGEHPPDLDLPENLVRAFHITQGLLKGEWGPDRRLCSG
  HDVSDGGLVTCLLEMAFAGNCGIEVDVPAPGIHALPVLFAEEPGLVLEVQEADVAGVLQRYQSAGLHCLE
  LGHTGEAGPQAMVRVSVNGTVVVEEPVGQLRALWEETSFQLDLLQAEPRCVTEEKQGLKERIGPSYCLPP
  TFPVASVPCKPGGPIPRVAILREEGSNGDREMADAFHLAGFEVWDVTMQDLCSGAVRLDTFRGVAFVGGF
  SYADVLGSAKGWAAAVTFNPQAREELGRFRRRPDTFSLGVCNGCQLLALLGWVGSDPNEDQVEPDHDSQP
  AQPGLLLRHNLSGRFESRWATVRVEPGPALMLRGMEGSVLPVWSAHGEGYMAFSSPELQAKIKAKGLVPL
  HWADDDGNPTEQYPLNPNGSPGGIAGICSPDGRHLALMPHPERAVRLWQWAWRPSPFDGLSTSPWLQLFI
  NARNWTQEDSC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001105791
• ORF Size: 3813 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001105791.1, NP_001099261.1
• RefSeq Size: 4488 bp
• RefSeq ORF: 3816 bp
• Locus ID: 287420
• Cytogenetics: 10q24
• MW: 136.9 kDa