Mtrex (NM_001034093) Rat Tagged ORF Clone

CAT#: RR202950

Skiv2l2 (Myc-DDK-tagged ORF) - Rat superkiller viralicidic activity 2-like 2 (S. cerevisiae) (Skiv2l2), (10 ug)

Product Images

Circular map for RR202950

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mtrex
• Synonyms: RGD1305984; Skiv2l2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202950 representing NM_001034093
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACGCTTTCGGGGATGAGCTTTTCAGCGTTTTCGAGGATGATTCGACCACTGCAGCGGGAACCA
  AGAAAGACAAAGAAAAGGAGAAAGATAAATGGAAGGGGCCACCGGGGCCTGCAGACAAGGCCGGGAAACG
  ACTGGATACCAGATTACAGTCAGAACCAGCCAGTAGTGGGAAAAACAAGAGAGATCTAGATGTTGAAGGC
  ACAGATGAACCTATTTTTGGCAAGAAGCCCAGGATAGAAGACTCAATTAATGAAGATTTAAGCTTGGCGG
  ACCTGATGCCCAGAGTCAAGGTACAGTCGGTTGAAACAGTTGAAGGCTGTACACACGAGGTCGCTCTTCC
  TGCAGATGAGGATTATATACCACTTAAGCCTCGAGTTGGGAAGGCAGCAAAGGAATACCCGTTCATTCTT
  GATGCTTTCCAAAGAGAAGCCATTCAGTGTGTTGATAACAATCAGTCTGTGTTAGTATCTGCTCATACAT
  CAGCGGGGAAAACCGTGTGCGCTGAGTATGCTATTGCACTGGCTTTAAGGGAAAAACAGCGTGTAATATT
  TACCAGCCCAATAAAGGCTCTAAGTAATCAGAAATACCGTGAAATGTATGAAGAATTTCAAGATGTTGGT
  CTGATGACTGGAGATGTTACTATTAATCCCACAGCATCTTGTCTGGTTATGACTACAGAGATTTTGAGAA
  GTATGCTGTACAGAGGCTCTGAAGTTATGCGAGAAGTTGCGTGGGTCATATTTGATGAAATTCATTACAT
  GAGAGATTCAGAGCGTGGTGTAGTATGGGAAGAAACTATTATTTTGCTTCCAGACAATGTTCACTATGTC
  TTTCTTTCGGCTACTATTCCCAATGCTCGACAGTTTGCTGAGTGGATCTGCCACCTACATAAACAGCCCT
  GTCATGTAATTTATACTGATTATCGACCTACTCCATTGCAACACTACATATTTCCAGCAGGGGGAGATGG
  CCTTCACCTTGTGGTTGATGAAAATGGTGACTTCAGAGAAGATAATTTTAACACTGCAATGCAAGTACTT
  CGGGATGCAGGTGATTTGGCCAAAGGAGACCAGAAGGGACGGAAAGGAGGAACAAAAGGTCCATCAAATG
  TGTTCAAGATTGTGAAGATGATTATGGAAAGAAATTTTCAACCTGTGATTATCTTCAGTTTTAGTAAGAA
  GGATTGTGAAGCATACGCCCTTCAAATGACTAAACTAGACTTCAACACAGATGAAGAAAAGAAGATGGTT
  GAAGAAGTTTTCAATAATGCAATTGACTGTTTGTCTGATGAAGATAAAAAGCTCCCTCAGGTTGAACATG
  TACTTCCACTTTTGAAACGGGGGATTGGTATTCACCATGGTGGTTTGCTTCCTATTTTAAAGGAAACTAT
  AGAGATTCTCTTTTCTGAAGGATTGATAAAGGCCTTATTTGCCACAGAGACTTTTGCTATGGGAATTAAC
  ATGCCAGCTAGAACTGTTTTATTTACAAATGCCCGTAAATATGATGGGAAGGATTTTAGATGGATTTCCT
  CAGGTGAGTACATCCAGATGTCTGGTCGTGCAGGAAGGAGGGGGATGGATGACAGAGGGATTGTGATTCT
  TATGGTCGATGAAAAGATGAGCCCAACAATTGGAAAACAACTGCTTAAGGGCTCAGCTGATCCTCTGAAT
  AGTGCTTTCCATCTAACGTACAACATGGTTTTGAACTTACTAAGAGTAGAAGAAATTAATCCCGAGTACA
  TGCTTGAAAAATCCTTCTACCAGTTTCAGCATTATAGAGCAATTCCAGGAGTTGTAGAAAAGGTAAAGAA
  CTCGGAAGAACAGTATAATAAAATAGTAATTCCCAATGAAGAAAATGTGGTCATCTATTACAAGATTAGA
  CAGCAACTTGCCAAGCTGGGAAAAGAGATTGAAGAATATATTCATAAGCCAAAATACTGCTTACCCTTTC
  TACAACCAGGCCGTTTAGTAAAGGTGAAGAATGAAGGTGATGATTTCGGCTGGGGAGTAGTGGTGAACTT
  CTCAAAAAAGTCAAACGTCAAGCCCAATTCTGGCGAACTGGATCCCTTGTATGTGGTGGAAGTGCTCCTG
  CGCTGTAGCAAGGAAAGCCTGAAAAACTCCGCCACAGAGGCAGCAAAGCCAGCAAAGCCGGATGAGAAGG
  GGGAGATGCAGGTTGTTCCGGTTTTGGTGCATCTCCTGTCTGCCATCAGCACTGTTAGACTCTACATTCC
  AAAAGACCTCCGCCCCGTGGACAACAGACAGAGTGTCCTAAAATCCATACAGGAAGTTCAAAAGCGGTTT
  CCCGATGGTGTTCCGTTGTTAGATCCTATCGATGATATGGGCATTCAAGATCAAGGACTGAAAAAAGTCA
  TTCAGAAAGTAGAGGCTTTTGAGCATCGAATGTACTCGCACCCACTTCACAATGATCCTAATCTGGAAAC
  TGTGTATACCCTTTGTGAAAAAAAGGCACAGATTGCAATAGATATTAAGTCTGCAAAGAGAGAACTGAAG
  AAAGCAAGAACTGTTCTGCAAATGGATGAACTCAAATGTCGTAAGCGTGTTCTAAGAAGGCTGGGTTTTG
  CTACATCTTCTGATGTCATAGAGATGAAAGGACGGGTGGCTTGTGAGATAAGCAGTGCTGACGAGCTCCT
  TCTAACAGAGATGATGTTTAATGGGCTCTTCAATGACCTTTCTTCAGAACAGGCGACCGCACTGCTAAGC
  TGTTTTGTGTTTCAAGAGAATTCTAGTGAGATGCCCAAATTAACAGAGCAGTTAGCTGGACCACTCCGAC
  AAATGCAGGAATGTGCTAAAAGGATTGCAAAGGTTTCTGCAGAGGCAAAACTGGAGATTGATGAAGAAAC
  TTACTTAAGCTCATTCAAGCCTCACCTGATGGATGTGGTGTACACCTGGGCAACTGGGGCCACATTTGCC
  CACATCTGCAAAATGACAGATGTCTTTGAAGGCAGTATAATCCGTTGTATGAGGCGCCTGGAAGAACTGC
  TTCGACAGATGTGTCAAGCAGCGAAAGCCATTGGAAACACAGAGCTGGAGAACAAGTTTGCTGAAGGAAT
  CACCAAAATCAAGAGGGATATTGTATTTGCTGCCAGCCTCTACTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202950 representing NM_001034093
  Red=Cloning site Green=Tags(s)
  
  MADAFGDELFSVFEDDSTTAAGTKKDKEKEKDKWKGPPGPADKAGKRLDTRLQSEPASSGKNKRDLDVEG
  TDEPIFGKKPRIEDSINEDLSLADLMPRVKVQSVETVEGCTHEVALPADEDYIPLKPRVGKAAKEYPFIL
  DAFQREAIQCVDNNQSVLVSAHTSAGKTVCAEYAIALALREKQRVIFTSPIKALSNQKYREMYEEFQDVG
  LMTGDVTINPTASCLVMTTEILRSMLYRGSEVMREVAWVIFDEIHYMRDSERGVVWEETIILLPDNVHYV
  FLSATIPNARQFAEWICHLHKQPCHVIYTDYRPTPLQHYIFPAGGDGLHLVVDENGDFREDNFNTAMQVL
  RDAGDLAKGDQKGRKGGTKGPSNVFKIVKMIMERNFQPVIIFSFSKKDCEAYALQMTKLDFNTDEEKKMV
  EEVFNNAIDCLSDEDKKLPQVEHVLPLLKRGIGIHHGGLLPILKETIEILFSEGLIKALFATETFAMGIN
  MPARTVLFTNARKYDGKDFRWISSGEYIQMSGRAGRRGMDDRGIVILMVDEKMSPTIGKQLLKGSADPLN
  SAFHLTYNMVLNLLRVEEINPEYMLEKSFYQFQHYRAIPGVVEKVKNSEEQYNKIVIPNEENVVIYYKIR
  QQLAKLGKEIEEYIHKPKYCLPFLQPGRLVKVKNEGDDFGWGVVVNFSKKSNVKPNSGELDPLYVVEVLL
  RCSKESLKNSATEAAKPAKPDEKGEMQVVPVLVHLLSAISTVRLYIPKDLRPVDNRQSVLKSIQEVQKRF
  PDGVPLLDPIDDMGIQDQGLKKVIQKVEAFEHRMYSHPLHNDPNLETVYTLCEKKAQIAIDIKSAKRELK
  KARTVLQMDELKCRKRVLRRLGFATSSDVIEMKGRVACEISSADELLLTEMMFNGLFNDLSSEQATALLS
  CFVFQENSSEMPKLTEQLAGPLRQMQECAKRIAKVSAEAKLEIDEETYLSSFKPHLMDVVYTWATGATFA
  HICKMTDVFEGSIIRCMRRLEELLRQMCQAAKAIGNTELENKFAEGITKIKRDIVFAASLYL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001034093
• ORF Size: 3126 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034093.1, NP_001029265.1
• RefSeq Size: 3582 bp
• RefSeq ORF: 3129 bp
• Locus ID: 365668
• Cytogenetics: 2q14
• MW: 117.9 kDa