Pfn1 (NM_022511) Rat Tagged ORF Clone
CAT#: RR202723
- TrueORF®
Pfn1 (Myc-DDK-tagged ORF) - Rat profilin 1 (Pfn1), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_022511" in other vectors (3)
USD 198.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Pfn1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR202723 representing NM_022511
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGGTGGAACGCCTACATCGACAGCCTTATGGCGGACGGGACCTGTCAGGACGCGGCCATCGTAG GCTACAAGGACTCGCCCTCCGTCTGGGCCGCTGTCCCCGGGAAGACCTTCGTTAGCATTACGCCAGCTGA GGTTGGTGTCCTGGTAGGCAAAGACCGGTCAAGTTTTTTCGTGAATGGGCTGACACTTGGGGGCCAGAAA TGTTCTGTGATCCGGGACTCACTGCTGCAAGACGGGGAATTTACAATGGATCTTCGTACCAAGAGCACCG GGGGAGCCCCCACCTTCAATGTCACTGTCACCATGACTGCCAAGACGCTAGTCCTGCTGATGGGCAAAGA AGGTGTCCACGGTGGTTTGATCAACAAGAAATGTTATGAAATGGCCTCTCACCTGCGGCGTTCCCAGTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202723 representing NM_022511
Red=Cloning site Green=Tags(s) MAGWNAYIDSLMADGTCQDAAIVGYKDSPSVWAAVPGKTFVSITPAEVGVLVGKDRSSFFVNGLTLGGQK CSVIRDSLLQDGEFTMDLRTKSTGGAPTFNVTVTMTAKTLVLLMGKEGVHGGLINKKCYEMASHLRRSQY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_022511 |
ORF Size | 420 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_022511.2, NP_071956.2 |
RefSeq Size | 862 bp |
RefSeq ORF | 423 bp |
Locus ID | 64303 |
UniProt ID | P62963 |
Cytogenetics | 10q24 |
MW | 15 kDa |
Gene Summary | regulates actin polymerization; may reorganize the actin cytoskeleton; may be involved in the pathogenesis of glomerulonephritis [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN202723 | Pfn1 (untagged ORF) - Rat profilin 1 (Pfn1), (10 ug) |
USD 165.00 |
|
RR202723L3 | Lenti ORF clone of Pfn1 (Myc-DDK-tagged ORF) - Rat profilin 1 (Pfn1), (10 ug) |
USD 465.00 |
|
RR202723L4 | Lenti ORF clone of Pfn1 (mGFP-tagged ORF) - Rat profilin 1 (Pfn1), (10 ug) |
USD 465.00 |
{0} Product Review(s)
Be the first one to submit a review