Plekhg4 (NM_001135870) Rat Tagged ORF Clone

CAT#: RR201981

Plekhg4 (Myc-DDK-tagged ORF) - Rat pleckstrin homology domain containing, family G (with RhoGef domain) member 4 (Plekhg4), (10 ug)

Product Images

Circular map for RR201981

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Plekhg4
• Synonyms: RGD1310790
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201981 representing NM_001135870
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGGCCTCTTGAGTGTGGGGATGTCTCCCTAGACTACCAGGGCCATGCTGCAGATGGGAGGAGGT
  TTGTTGTGTGCAGTTTCAGGAACACCGGGAAGGAGGAGGAACCTGCTCACCAGATACAGGCTATGGACCC
  AAGACTTTCAAGACCAGCAGTAGGGGCAACCCAAGGCATAGGGCTACAAGGAGATTTCTTATCCAAGAAG
  CCTCAATCACTTCCAGATCAAAGCGCTGCAGATAGGTCAGGGGGCAACCAGAGGAGCATATTAGGAGCCC
  CCCAAGCCCAGTCAGAGGAGCCAGCCTTCTCTGAAATGGAAAACTGTTTGTGTCCTGGTTCCTCCCACCT
  CAACCTGACACAGGGTGAGAATGACCATCGAGGGGGAGGATTGATAGGGGACCCAGAGCCAGGCAGAGCG
  CTGCCAGCTGGCTTTAGCCCTTTATTGGAGATGTCCTCAAAGCTACCGGAGGCAGACCCTAGTGGATCCA
  GCTTCCCTAGGCCTGCTGACTGCCTCCTAGCTCGAGATCTCACCTGGGAGCTACTGGCCAGCGGCATGGC
  TGCCCTCCCAGGGACACGGGACATAGAAGGCCGAGCAGTGCTACTTTTGTGTGCCCACAGCCCAGCTTGG
  CTTCATCCCAAGTGTAATAGCCATGAGCTGCTGAGTCTTCTGTTCTACCTACGAGGCATCCCCAGGCCTG
  AGGTGCAGGCTCTGGGACTGACCGTACTAGTGGATGCCCGAATCTGCCCACCGAGCTCTGCCCTCACCTG
  GGGCCTCAGCCAACTACAAGAAGCATCCCCAGGGTCTGTACAGCAACTGCTGCTGGTTGGGAAGATGCCC
  GAGGACATGCCCGTGGGGCTCCAGGCCAAGCAGCTGGCTTCTCATCAGAGCCTCCTCACCCACATTCCTA
  ACATGGAGTTGCCTACTTCACTAGGAGGATGCCTGTCTTACTGCCACCAAGCCTGGCTGGATTTCCGGAT
  GCGTCTGGAAACCCTACAGCAGAGTTGTCAGGTGGCTTGTGCCCTGCTTCAGGGCGTCATTGACAGTATG
  AAGGCTATGCGGCAGCCCTTGGAGTCTGGAGAAGTTGGTGAGCTGCTACAGCAGGCACAGTCCCTCATGC
  AGCAGGTATTAGAGTCACCACAGTTGTCATGGTTACAAAGTCAGGGAAGCCTGGAGCTCGCATGGTTGAA
  ACAAGGGATGCCACAGGTGGCCCTGAGCCCCGATTACAGGTCTGCAGTAGACAAGGCTGATGAGTTGTAT
  GGTCAAGTGGATGAACTGTTGCATCGGCTAACCCTGAAGAGCAACCAGCGAATCCGGGCCCTAGAGCTCC
  TCCAAGCACTGGAAGCCCAGGAGGGTGTGCTGTGCCAGATTGAAGTGTGGCTACAGGAGGTGGGCTGGCC
  AGGGCTGGAGGAACCAGGGGAGCCCTCACTGGACATGCTACTCCAGGCCCAGGGCCCTTTTCAAGAGCTG
  GACCTGGTTGCCCAGGAACAGGTCAAGCAAGGGGAGAAGTTACTGCAGCCATTAGTTGGCTGGGAGGCTG
  CTGAACTGGGGCCCACTGGAAAGCGCTTTCTGGCCCTGAGATCCCAGCTGATGGAATTTTCCAGAGCTTT
  GGCCCAGCGGCGCCAGCGGTTGGCAGATGCTGAGAAGCTGTTTCAGTTCTTTAAGCAGGCCTCAACATGG
  ACGGAGGAAGGGCAGCGGGTGCTGACAGAGCTGGAGCAGGAGACCCCGGAGGTTGTGCTGCAGCGACTGC
  AGCTGCACTGGAGCAAACACCCTGACTTGCCTCCTGCCCACTTCCGGAAAATGTGGGCTCTGGCTACAGG
  CCTAGGCTCAGAAGGCATTCGCCAGGAATGTCGCTGGGCCTGGGCACAGTGCCAGGACACCTGGCTGGCT
  CTGGATCAGAAGCGTGAGGCTGCACTAAAGCCACAATCAATGAATAGCACAGCTACTTTGTATGTCAGGC
  GGGCTCCTGCTATACCCACCATCCCTCCACTGAGGAAGTCCTACAGCTTTGATCAGAATCTGGGGAAGCA
  TCTTGGAGATGCTTCCAATCGTGGCCACTGTGCAGCCACTGTTACTGACTGCCACAGACCTGAGGCCAGA
  GGAGGCATCCGGCCCAGGTCATCACCTTCTGTGCCTCTGTCAGGCAGTTCTGACTTCAGGAGCCCCAACA
  GGCTACAGCTAGTATTGGCGGAGATGGTGGCCACAGAGCGTGAGTATGTCCGGGCCCTTGACTACACCAT
  ACAGAACTACTTCCCTGAGTTAGATCGGTCCGATGTGCCCCAGGGCCTCCGTGGCCAGCGTGCACACCTC
  TTTGGCAACCTTGAAAAGCTTCGGGATTTCCACTTCCATTTCTTCCTGCGTGAGCTAGAGGCTTGCACCC
  GGCACCCACCTCGAGTGGCTCATGCCTTCCTGCGCCACAGGGTGCAGTTTGGAATGTATGCACTCTACAG
  CAAGAATAAACCTCGGTCTGATGCCCTGATGAGCAACTATGGCCACACCTTCTTCAAGGAGAAGCAGCAG
  GCACTGGGAGACCACCTGGACTTGGCTTCCTACCTGCTAAAGCCCATCCAGCGCATGAGCAAGTATGCCT
  TACTGCTGCAGGAGCTGGCGAGGGCCTGTGGGGGTCCAGCACAAGAGCTGGGTGCCCTTCAGGCAGCCCA
  GAGCCTTGTGCATTTCCAGCTGAGACATGGCAATGACCTGCTAGCCATGGATGCCATTCAGGGCTGTGAC
  GTTAACCTCAAGGAGCAGGGGCAGTTGGTACGACAGGATGAGTTCACAGTGCGGGCTGGGCACCACAAGT
  CCTGTCGTCGCGTTTTCCTGTTTGAGGAGCTGCTGCTCTTCAGCAAACCTCGACGTGGGCCTGCAGGTGT
  TGACATATTCACCTACAAGCGTTCCTTCAAAATGGCAGACCTCGGCCTCACGGAGTGCTGTGGGGAAAGT
  AAACTGCGCTTCGAGATCTGGTTCCGACGTCGCAAGGCCAGGGACCTGTTTGTGCTTCAGGCTTCCGATG
  TGGCCACCAAACAAGCCTGGACAGCTGATATCTCCCGTCTCCTCTGGAGGCAGGCTGTACACAACAAGGA
  GGTGCGCATGGCTGAGATGGCATCCATGGGTGTGGGGAACAAAGCCTTCTGGGACATTGCCCCCAGTGAG
  GAGGCTATCAATGACCGCAACATCAACTATGTGCTGAAAAGACGAGATGTTCGCTCTAGGGCCTCCATTG
  CTGTGGCCCCATTTGACTATGACAACCCTTATCTGGGTGCCTTGGCCTCCCTACCTGGAGACCGTGCCTC
  TTGCTCTGTTCTGGGGTCTCTCAATCTGCACCTGTACAGAGATCCAGCTCTTCTGGGTGTTCACTGGTCC
  TTGCTTCCACCCAACTTCCCAGAAGAAGCATCACTAGATACTGAGGTGGACTTGGGCAGCCAGCCTTCTT
  TGACTCCTGAGGACTCAGAGGTCTCATCCCAGTGCCCATCAGCCAGTGGCTCTAGTGGTTCTGACAGCAG
  CTGTGTGTCGGGGCAGACCATGGGCAGAGGCCTTGAAGACTTGTCCTGTTTTTTCAGTCTGTGTGTCGGG
  GCAGACCATGGGCAGAGGCCTTGAAGACTTGTCCTGTTTTTTCAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201981 representing NM_001135870
  Red=Cloning site Green=Tags(s)
  
  MEGPLECGDVSLDYQGHAADGRRFVVCSFRNTGKEEEPAHQIQAMDPRLSRPAVGATQGIGLQGDFLSKK
  PQSLPDQSAADRSGGNQRSILGAPQAQSEEPAFSEMENCLCPGSSHLNLTQGENDHRGGGLIGDPEPGRA
  LPAGFSPLLEMSSKLPEADPSGSSFPRPADCLLARDLTWELLASGMAALPGTRDIEGRAVLLLCAHSPAW
  LHPKCNSHELLSLLFYLRGIPRPEVQALGLTVLVDARICPPSSALTWGLSQLQEASPGSVQQLLLVGKMP
  EDMPVGLQAKQLASHQSLLTHIPNMELPTSLGGCLSYCHQAWLDFRMRLETLQQSCQVACALLQGVIDSM
  KAMRQPLESGEVGELLQQAQSLMQQVLESPQLSWLQSQGSLELAWLKQGMPQVALSPDYRSAVDKADELY
  GQVDELLHRLTLKSNQRIRALELLQALEAQEGVLCQIEVWLQEVGWPGLEEPGEPSLDMLLQAQGPFQEL
  DLVAQEQVKQGEKLLQPLVGWEAAELGPTGKRFLALRSQLMEFSRALAQRRQRLADAEKLFQFFKQASTW
  TEEGQRVLTELEQETPEVVLQRLQLHWSKHPDLPPAHFRKMWALATGLGSEGIRQECRWAWAQCQDTWLA
  LDQKREAALKPQSMNSTATLYVRRAPAIPTIPPLRKSYSFDQNLGKHLGDASNRGHCAATVTDCHRPEAR
  GGIRPRSSPSVPLSGSSDFRSPNRLQLVLAEMVATEREYVRALDYTIQNYFPELDRSDVPQGLRGQRAHL
  FGNLEKLRDFHFHFFLRELEACTRHPPRVAHAFLRHRVQFGMYALYSKNKPRSDALMSNYGHTFFKEKQQ
  ALGDHLDLASYLLKPIQRMSKYALLLQELARACGGPAQELGALQAAQSLVHFQLRHGNDLLAMDAIQGCD
  VNLKEQGQLVRQDEFTVRAGHHKSCRRVFLFEELLLFSKPRRGPAGVDIFTYKRSFKMADLGLTECCGES
  KLRFEIWFRRRKARDLFVLQASDVATKQAWTADISRLLWRQAVHNKEVRMAEMASMGVGNKAFWDIAPSE
  EAINDRNINYVLKRRDVRSRASIAVAPFDYDNPYLGALASLPGDRASCSVLGSLNLHLYRDPALLGVHWS
  LLPPNFPEEASLDTEVDLGSQPSLTPEDSEVSSQCPSASGSSGSDSSCVSGQTMGRGLEDLSCFFSLCVG
  ADHGQRP*RLVLFFQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001135870
• ORF Size: 3558 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 3561 bp
• RefSeq ORF: 3561 bp
• Locus ID: 307796
• Cytogenetics: 19q12
• MW: 131.7 kDa