Mme (NM_012608) Rat Tagged ORF Clone

CAT#: RR201833

Mme (Myc-DDK-tagged ORF) - Rat membrane metallo endopeptidase (Mme), (10 ug)

Product Images

Circular map for RR201833

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mme
• Synonyms: CD10; Nep; SFE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201833 representing NM_012608
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAGATCAGAAAGTCAGATGGATATTACTGATATCAATGCTCCAAAGCCGAAGAAGAAACAGCGAT
  GGACTCCACTGGAGATCAGCCTTTCTGTGCTCGTCTTGCTCCTGACTATCATAGCTGTGACAATGATTGC
  TCTCTATGCAACCTATGATGATGGTATTTGCAAATCATCAGACTGCATAAAATCAGCTGCTCGACTGATC
  CAGAACATGGATGCCTCTGCTGAGCCATGTACGGACTTCTTCAAATATGCTTGTGGAGGCTGGTTGAAAC
  GCAATGTCATCCCTGAGACCAGTTCCCGATACAGTAATTTTGACATTCTAAGAGATGAACTAGAAGTCAT
  TTTGAAAGATGTCCTTCAAGAACCCAAAACTGAGGACATAGTAGCAGTGCAGAAAGCAAAAACTTTGTAC
  AGATCATGTATAAATGAATCTGCTATTGATAGCAGAGGTGGGCAACCTCTGCTCACACTGTTACCAGATA
  TATATGGGTGGCCAGTAGCATCACAAAACTGGGAACAAACATATGGTACTTCTTGGACAGCTGAGAAATC
  TATTGCACAACTGAATTCTAAATATGGGAAAAAGGTCCTCATTAATTTTTTTGTTGGCACTGATGATAAG
  AATTCTACCCAGCATATAATTCATTTTGACCAGCCTCGACTTGGCCTCCCTTCCAGAGACTACTATGAGT
  GTACAGGAATATATAAAGAGGCTTGCACAGCATATGTGGATTTTATGATTTCTGTGGCCAGACTGATTCG
  TCAGGAACAAAGATTGCCTATTGATGAAAACCAGCTCTCTTTGGAAATGAATAAAGTTATGGAATTGGAA
  AAAGAAATTGCCAATGCCACAACTAAACCAGAAGACCGAAATGACCCAATGCTGCTTTATAACAAAATGA
  CATTGGCCAAGCTCCAAAATAACTTCTCTCTGGAGATCAATGGGAAGCCATTCAGCTGGTCAAATTTCAC
  AAATGAAATCATGTCAACTGTGAATATTAATATTCAAAATGAGGAAGAAGTGGTTGTTTATGCTCCAGAA
  TATTTAACCAAACTTAAGCCTATTCTTACCAAATATTCTCCCAGAGATCTTCAAAATTTAATGTCCTGGA
  GGTTCATAATGGATCTTGTAAGCAGCCTCAGCCGAAACTACAAGGAGTCCAGAAATGCTTTCCGCAAGGC
  CCTTTACGGGACTACATCCGAAACTGCAACCTGGAGACGGTGTGCCAACTACGTCAATGGGAACATGGAG
  AATGCTGTGGGGAGGCTTTATGTGGAAGCAGCTTTTGCTGGAGAGAGCAAGCACGTGGTTGAAGATTTGA
  TTGCACAAATCCGTGAAGTTTTTATTCAGACTTTAGATGACCTCACTTGGATGGATGCTGAGACAAAAAA
  GAAAGCTGAAGAGAAGGCCCTGGCAATTAAAGAAAGGATTGGCTATCCTGATGACATCATCTCCAATGAG
  AATAAACTGAATAATGAGTATCTTGAGTTGAACTACAAGGAAGAGGAATACTTTGAGAACATAATTCAAA
  ATTTGAAATTCAGCCAAAGCAAGCAGCTAAAGAAGCTCCGAGAAAAGGTGGACAAAGATGAGTGGATAAG
  TGGCGCGGCGGTAGTCAATGCATTTTATTCCTCAGGCAGAAATCAGATCGTCTTCCCCGCCGGCATTTTG
  CAGCCCCCATTCTTTAGTGCTCGGCAGTCCAACTCATTGAACTATGGGGGCATCGGCATGGTCATCGGAC
  ATGAAATCACACATGGCTTTGATGACAATGGCAGAAATTTTAACAAAGATGGAGACCTCGTTGACTGGTG
  GACTCAGCAGTCTGCAAATAATTTCAAAGACCAATCCCAGTGTATGGTGTACCAGTATGGAAACTTTACA
  TGGGACCTAGCAGGTGGACAGCATCTCAATGGAATTAACACACTAGGAGAAAATATTGCTGATAATGGAG
  GGATTGGCCAAGCATACAGAGCCTATCAGAATTATGTTAAAAAGAATGGTGAAGAAAAATTACTCCCTGG
  ACTTGACCTCAATCACAAACAACTATTCTTCTTGAACTTTGCCCAGGTGTGGTGTGGAACCTACCGGCCA
  GAGTATGCAGTCAATTCCATTAAAACAGATGTACACAGTCCTGGCAATTTCAGGATCATTGGGACTTTGC
  AGAACTCTGCTGAGTTTGCGGATGCCTTTCATTGCCGCAAGAACTCATACATGAATCCAGAAAGGAAATG
  TCGGGTTTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201833 representing NM_012608
  Red=Cloning site Green=Tags(s)
  
  MGRSESQMDITDINAPKPKKKQRWTPLEISLSVLVLLLTIIAVTMIALYATYDDGICKSSDCIKSAARLI
  QNMDASAEPCTDFFKYACGGWLKRNVIPETSSRYSNFDILRDELEVILKDVLQEPKTEDIVAVQKAKTLY
  RSCINESAIDSRGGQPLLTLLPDIYGWPVASQNWEQTYGTSWTAEKSIAQLNSKYGKKVLINFFVGTDDK
  NSTQHIIHFDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEQRLPIDENQLSLEMNKVMELE
  KEIANATTKPEDRNDPMLLYNKMTLAKLQNNFSLEINGKPFSWSNFTNEIMSTVNINIQNEEEVVVYAPE
  YLTKLKPILTKYSPRDLQNLMSWRFIMDLVSSLSRNYKESRNAFRKALYGTTSETATWRRCANYVNGNME
  NAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNE
  NKLNNEYLELNYKEEEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGIL
  QPPFFSARQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVYQYGNFT
  WDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRP
  EYAVNSIKTDVHSPGNFRIIGTLQNSAEFADAFHCRKNSYMNPERKCRVW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012608
• ORF Size: 2250 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012608.2, NP_036740.1
• RefSeq Size: 3493 bp
• RefSeq ORF: 2253 bp
• Locus ID: 24590
• UniProt ID: P07861
• Cytogenetics: 2q31
• MW: 85.8 kDa
• Gene Summary: zinc-containing metallo-enzyme; involved in degradation of cardiac peptides [RGD, Feb 2006]