Tacc1 (NM_001004107) Rat Tagged ORF Clone

CAT#: RR201768

Tacc1 (Myc-DDK-tagged ORF) - Rat transforming, acidic coiled-coil containing protein 1 (Tacc1), (10 ug)

Product Images

Circular map for RR201768

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tacc1
• Synonyms: Tacc1a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201768 representing NM_001004107
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTTCAGTCCCTGGCAGATCCTGTCGCCCGTGCAGTGGGCCAAGTGGACGTGGTCCGCGGTGCGCG
  GTTCGGGCGCGGGAGAGGACGAAGCTGGAGGTCCCGAAGGCGACCCCGAGGAGGAAGAGGACTCGCAAGC
  CGAGACCAAATCCCTGAGTTTCAGCTCGGATTCTGAAGGTAATTTTGAGACTCCTGAAGCTGAAACTCCA
  ATCAGATCGTCTCTCAAGGAGTCTTGTGATTCATCACTAGGATTGTCAGAACCCGAGGCCAAACCCCAAG
  AATCACGAGAAGCCGATGAACAGCCGGTAGCAGAATTAATTGAAAAATGCTCGCCTGATGCGTGTTCTAG
  ATCTTCAGGAAATGAAGCGCCACAGGCCACTATCGATTCTTACTCAGTCAAGGATGTCAGAGGGAAGGCG
  GAACATGATGTTAGCAAGATCTCAGTAGTGAGGCCGTTTTCCATAGAAACCAGGGACTGCACAGATGACC
  CTGCTGCTCATGGCTGTGTCCCTGTGCTCCCAGGCAAGGCCTTGCCTTCCACCACACCCGAAGCCACTCA
  GGACCAGCCAATGATGGACAGAGGCATGGGGGTTACATTGGAGGCTTTTACAGAAGTCAATCTGAAGACT
  GGAGGCCCCTGCCCAGAGCCTGTGCCCAGCAGAAGCAGGCTAAGAAAGCCCAAGCCTGTCTCCCTGAGGA
  AGAAGACAGCTGGGGACAACACCTTTGAACCCGAAATGTTGATGGAGGGTTCACTTCTCCCTAAGGCTTC
  CTCCCCGTGGATTCCTGAGGAGTCTGATAAGAACTCGAATCCTAGTTTGCTGAGAGGCTTGAGGGCCCAC
  AAATCTCCCCTTAACCTTAAAGAAGCTGCTAGCATTCACTGTAACAGCGCCGGTGACTCGGGGGTTGATC
  TGCAGGAGGGGTCCAGGGCCTCACCTCTCAAACTTGATGATTTTACAGAAGACGGGCGGAGTGTCGAGAC
  CAGGAGTGCCCTTCCGAAGCAGCCTGGCAGGAAACCCAGCCACAAACTGGCACCCAATATACGAAAAGAT
  GGCTCCAGTAAGCCAGTAGGTGTTGAGCAGCTCACAGACCCAACCATTGAAGATGCCTCCCTCGCCCAGA
  CGTCTCCCAAGTTAGACCCAAGTAAATGGGGTCGTCCTAACTTTAATTCCTTCGGGAGCTGCCCCATTTT
  GCAGAGCTCCCCGCCCGTCTCCTCCAAAGGTTCCTACCACCTTGACCCAGATAACGTTAACGCCGATGAA
  TCCGGGGACCCCTGTAAGCCGGCGACAGCTTTGACAGGCAGCGGCTTTTGCTCTGCCACTGGTAATCATG
  TTAATGAAATCTTAGACTCACCCAAGAAGGCACAGTCGCGTTTAATAACGAGTGGCTGCAAGGTGAAGAA
  ATACGAAACTCAGTCTCTTGATTTGGACGCTTGTTCTCAGGATGAAGGAGCCGTGATCTCACAGATTGCA
  GACATTCCTAATCGGGACGGGCACGCCACCGATGAGGAGAAGCTGGCGTCCACGTCGTGTAGTCAGAAGT
  CAGCCGGGGCTGAAGTGAAAGGCGTAGAAAAGGAGGCATACCAGAAGATGGAGGAGGAGGAGCTGACTGT
  GCATGGACTGCTGGAGACCTCTTCAGAAAAGGCCCCCGTGTCTGTGGCCTGTGGAGGTGAGAGCCCTCTG
  GATGGGATCTGCCTCAGTGAAGCAGACAAGACTGCTGTGCTTACATTGATCAGAGAAGAGATCATCACGA
  AAGAGATTGAAGCAAATGAATGGAAGAAGAAATACGAAGAGACCCGAGAAGAAGTCCTGGAGATGAGGAA
  AATTGTGGCAGAATATGAAAAGACCATTGCCCAAATGATCGAAGACGAGCAGAGGACAAACATGAGCTCT
  CAGAAGAGTTTCCAGCAGCTGACTATGGAGAAGGAGCAGGCTCTGGCTGACCTGAACTCTGTGGAAAGGT
  CCCTTTCTGATCTCTTCAGAAGATATGAAAACCTGAAAGGCGTGCTAGAAGGGTTCAAGAAGAATGAAGA
  AGCCTTGAAAAAGTGTGCTCAGGATTACTTAGCCAGAGTGAAGCAGGAAGAGCAGCGCTACCAGGCCCTG
  AAGGTCCACGCTGAGGAAAAGCTGGACAGAGCCAACGAGGAAATCGCTCAGGTTCGGTCCAAGGCGAAGG
  CTGAGAGTGCGGCTCTCCATGCTGGACTCCGGAGGGAGCAGATGAAGGTGGAGTCCCTGGAGAGGGCCCT
  TCAGCAGAAGAATCAAGAGATTGAAGAACTGACAAAAATCTGTGACGAGTTGATCGCAAAGCTTGGAAAG
  GCCGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201768 representing NM_001004107
  Red=Cloning site Green=Tags(s)
  
  MAFSPWQILSPVQWAKWTWSAVRGSGAGEDEAGGPEGDPEEEEDSQAETKSLSFSSDSEGNFETPEAETP
  IRSSLKESCDSSLGLSEPEAKPQESREADEQPVAELIEKCSPDACSRSSGNEAPQATIDSYSVKDVRGKA
  EHDVSKISVVRPFSIETRDCTDDPAAHGCVPVLPGKALPSTTPEATQDQPMMDRGMGVTLEAFTEVNLKT
  GGPCPEPVPSRSRLRKPKPVSLRKKTAGDNTFEPEMLMEGSLLPKASSPWIPEESDKNSNPSLLRGLRAH
  KSPLNLKEAASIHCNSAGDSGVDLQEGSRASPLKLDDFTEDGRSVETRSALPKQPGRKPSHKLAPNIRKD
  GSSKPVGVEQLTDPTIEDASLAQTSPKLDPSKWGRPNFNSFGSCPILQSSPPVSSKGSYHLDPDNVNADE
  SGDPCKPATALTGSGFCSATGNHVNEILDSPKKAQSRLITSGCKVKKYETQSLDLDACSQDEGAVISQIA
  DIPNRDGHATDEEKLASTSCSQKSAGAEVKGVEKEAYQKMEEEELTVHGLLETSSEKAPVSVACGGESPL
  DGICLSEADKTAVLTLIREEIITKEIEANEWKKKYEETREEVLEMRKIVAEYEKTIAQMIEDEQRTNMSS
  QKSFQQLTMEKEQALADLNSVERSLSDLFRRYENLKGVLEGFKKNEEALKKCAQDYLARVKQEEQRYQAL
  KVHAEEKLDRANEEIAQVRSKAKAESAALHAGLRREQMKVESLERALQQKNQEIEELTKICDELIAKLGK
  AD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004107
• ORF Size: 2316 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004107.3, NP_001004107.1
• RefSeq Size: 7632 bp
• RefSeq ORF: 2319 bp
• Locus ID: 306562
• Cytogenetics: 16q12.4
• MW: 84.3 kDa
• Gene Summary: This gene encodes a member of the transforming, acidic coiled-coil (TACC) family of proteins. Members of this family are centrosomal proteins that interact with microtubules and tubulin. TACC proteins are thought to be involved in centrosome/mitotic spindle dynamics and gene regulation. [provided by RefSeq, Jul 2008]