Setd7 (NM_001109558) Rat Tagged ORF Clone

CAT#: RR201698

Setd7 (Myc-DDK-tagged ORF) - Rat SET domain containing (lysine methyltransferase) 7 (Setd7), (10 ug)

Product Images

Circular map for RR201698

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Setd7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201698 representing NM_001109558
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAGCGACGACGAGGTAGTGGAGGAAGCCGTGGAAGGGCACCTGGACGATGACGGATTACCACACG
  GGTTCTGCACTGTCACCTACTCCTCCACAGACAGATTTGAGGGCAACTTCGTGCATGGAGAAAAGAATGG
  ACGTGGGAAATTCTTCTTCTTTGACGGCAGCACCCTGGAGGGATACTATGTGGATGATGCCCTGCAGGGC
  CAGGGCGTTTACACCTATGAGGATGGAGGTGTTCTGCAAGGCACCTATGTGGATGGGGAGCTGAATGGGC
  CAGCCCAGGAGTACGACACCGACGGGAGGCTGATCTTCAAGGGACAGTACAAAGACAACATTCGACACGG
  AGTGTGTTGGATCCATTACCCAGATGGAGGCAGTCTCGTCGGGGAAGTCAATGAAGATGGGGAGATGACA
  GGGGAAAAGATAGCCTACGTGTACCCTGATCAGAGGACTGCGCTCTATGGGAAGTTCATTGATGGAGAGA
  TGCTGGAAGGGAAGCTGGCCACCCTCATGGCCACAGAAGAAGGGAAGCCACACTTTGAACTGACATCGGG
  AAGTTCCGTGTTCCACTTTGATAAATCGACTTCCTCCTGCATCTCTAGCGATGCCCTCCTTCCAGACCCT
  TATGAGTCCGAGAGGGTTTATGTTGCCGACTCTCTCATCTCCAGTGCCGGAGAAGGACTGTTTTCCAAGG
  TAGCAGTGGGACCCAATACTGTCATGTCATTTTATAATGGAGTCCGAATTACACACCAAGAGGTTGACAG
  CAGGGACTGGGCCCTAAATGGGAACACTCTCTCCCTGGATGAGGAAACAGTCATTGATGTGCCTGAGCCC
  TACAACCACGTGTCCAAGTACTGTGCCTCCTTGGGACACAAGGCGAATCACTCCTTTACTCCAAACTGCG
  TCTATGACATGTTTGTCCATCCCCGTTTTGGGCCCATCAAGTGCATCCGCACCCTTCGAGCTGTGGAGGC
  TGAGGAGGAGCTGACCGTTGCCTACGGCTATGACCACAGCCCCCCGGGGAAGAGTGGGCCCGAAGCCCCC
  GAGTGGTATCAGGTGGAGCTGAAGGCCTTCCAGGCCACCCAGCAAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201698 representing NM_001109558
  Red=Cloning site Green=Tags(s)
  
  MDSDDEVVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQG
  QGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQYKDNIRHGVCWIHYPDGGSLVGEVNEDGEMT
  GEKIAYVYPDQRTALYGKFIDGEMLEGKLATLMATEEGKPHFELTSGSSVFHFDKSTSSCISSDALLPDP
  YESERVYVADSLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEP
  YNHVSKYCASLGHKANHSFTPNCVYDMFVHPRFGPIKCIRTLRAVEAEEELTVAYGYDHSPPGKSGPEAP
  EWYQVELKAFQATQQK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001109558
• ORF Size: 1098 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001109558.1, NP_001103028.1
• RefSeq Size: 1977 bp
• RefSeq ORF: 1101 bp
• Locus ID: 689954
• Cytogenetics: 2q26
• MW: 40.5 kDa