Galns (NM_001047851) Rat Tagged ORF Clone

CAT#: RR201689

Galns (Myc-DDK-tagged ORF) - Rat galactosamine (N-acetyl)-6-sulfate sulfatase (Galns), (10 ug)

Product Images

Circular map for RR201689

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Galns
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201689 representing NM_001047851
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGCCTGTTCCACAGCGATCAGGGCCCAGCAGCTACTGCTGCCGGTACTGAGTGCTCTAGGACTCT
  TGGCTGCAGGTGCTCCGCAGCCCCCCAACATTGTGCTACTGCTCATGGACGATATGGGGTGGGGTGACCT
  GGGTGTGTATGGAGAACCTTCCAGAGAGACCCCAAATTTAGACCGGATGGCTGCAGAAGGGATGCTTTTC
  CCAAGCTTCTATTCTGCCAACCCTTTGTGCTCACCATCTAGGGCAGCCCTGCTCACAGGACGGTTGCCCA
  TCCGCAATGGCTTCTACACAACCAACGCACATGCAAGGAATGCTTACACACCTCAGGAGATCATGGGCGG
  GATCCCTAACTCAGAACACCTCCTGCCTGAGCTCCTGAAGAAAGCAGGCTATACCAACAAGATCGTGGGC
  AAATGGCATCTGGGTCATAGACCCCAGTTCCACCCCCTGAAGCATGGATTTGATGAGTGGTTTGGATCCC
  CCAATTGTCATTTTGGACCATACGACAACAAGGTCAAGCCCAACATCCCTGTGTACAGGGACTGGGAAAT
  GGTTGGCAGATTTTATGAAGAGTTCCCAATCAACCTAAAGACCGGGGAAGCTAACCTCACCCAACTCTAC
  TTACAAGAAGCTCTGGACTTCATCCGGACACAACATGCAAGGCAGAGCCCCTTCTTCCTCTACTGGGCCA
  TCGACGCCACACACGCACCAGTGTATGCCTCAAAACAGTTCCTGGGTACCAGCCTTCGAGGGCGTTATGG
  CGATGCTGTCCGGGAAATAGATGACAGTGTTGGAAAGATCCTGAGCCTTCTGCAGAACTTGGGCATCAGT
  AAGAACACATTTGTCTTCTTTACGTCTGACAATGGTGCAGCGCTCATCTCTGCTCCCAAAGAAGGTGGCA
  GCAATGGTCCCTTCCTGTGTGGGAAGCAGACTACGTTTGAAGGCGGGATGAGGGAGCCTGCAATCGCTTG
  GTGGCCAGGGCACATTGCCGCAGGCCAGGTCAGCCACCAGCTGGGAAGCATCATGGACCTCTTCACCACC
  AGCCTGTCCCTTGCAGGCCTGAAGCCCCCCAGTGACAGGGTAATTGATGGCCTTGACCTCCTCCCCACCA
  TGCTCCAGGGCCACATCATAGACAGGCCCATATTTTATTACCGTGGCAACACACTGATGGCAGTCACTCT
  TGGCCAGTACAAAGCACACCTCTGGACTTGGACCAACTCCTGGGAGGAGTTCAGACAGGGCATTGACTTC
  TGCCCCGGGCAGAATGTTTCAGGAGTCACAACCCACACCCAGGAAGAGCACACGGAGCTACCCCTGATCT
  TCCACCTGGGACGTGACCCAGGGGAGAGATTCCCACTCAGGTTCACCAGCAATGAGTACCAGGATGCCCT
  CAGCAGGACCACCCAGGTCATCCAGCAACACCAAAAGTCTTTGGTCCCTGGACAGCCCCAGCTCAATGTG
  TGTAACCAGGCTGTCATGAATTGGGCGCCTCCAGGCTGTGAAAAACTAGGGAAGTGTCTGACACCTCCTG
  AGTCTGTCCCCGAGAAGTGTTTCTGGGCCCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201689 representing NM_001047851
  Red=Cloning site Green=Tags(s)
  
  MTACSTAIRAQQLLLPVLSALGLLAAGAPQPPNIVLLLMDDMGWGDLGVYGEPSRETPNLDRMAAEGMLF
  PSFYSANPLCSPSRAALLTGRLPIRNGFYTTNAHARNAYTPQEIMGGIPNSEHLLPELLKKAGYTNKIVG
  KWHLGHRPQFHPLKHGFDEWFGSPNCHFGPYDNKVKPNIPVYRDWEMVGRFYEEFPINLKTGEANLTQLY
  LQEALDFIRTQHARQSPFFLYWAIDATHAPVYASKQFLGTSLRGRYGDAVREIDDSVGKILSLLQNLGIS
  KNTFVFFTSDNGAALISAPKEGGSNGPFLCGKQTTFEGGMREPAIAWWPGHIAAGQVSHQLGSIMDLFTT
  SLSLAGLKPPSDRVIDGLDLLPTMLQGHIIDRPIFYYRGNTLMAVTLGQYKAHLWTWTNSWEEFRQGIDF
  CPGQNVSGVTTHTQEEHTELPLIFHLGRDPGERFPLRFTSNEYQDALSRTTQVIQQHQKSLVPGQPQLNV
  CNQAVMNWAPPGCEKLGKCLTPPESVPEKCFWAH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001047851
• ORF Size: 1572 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001047851.1, NP_001041316.1
• RefSeq Size: 1575 bp
• RefSeq ORF: 1575 bp
• Locus ID: 292073
• UniProt ID: Q32KJ6
• Cytogenetics: 19q12
• MW: 58.3 kDa