Usp10 (NM_001034146) Rat Tagged ORF Clone

CAT#: RR201548

Usp10 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 10 (Usp10), (10 ug)

Product Images

Circular map for RR201548

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Usp10
• Synonyms: MGC124997
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201548 representing NM_001034146
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTCCACAACCCACAGTATATTTTTGGCGATTTCAGCCCTGATGAATTCAATCAGTTTTTTGTGA
  CTCCCCGCTCTTCAGTCGAGCTCCCTCCATACAGTGGGACTCAGTGTGGCATTCAGGCTGAAGAGGAACT
  TCTGGACGGACAAGAACATCAGAGGATTGAGTTTGGTGTAGACGAAGTCATCGAACCCAGTGATGGTCTG
  CAGAGAGCCCCTAGCTACAGTATTTCAAGCACCTTGAACCCTCAGGCACCTGAATTTATCCTTGGTTGTC
  CAACTTCCAAGAAGACCCCTGATGACATAGAAAAAGACGAGACCTACAGCTCCATTGATCAGTACCCGGC
  CTCAGCCTTGGCTCTGGAAAGCAGCTCTAACGCAGAGGCCGAGACCTTGGAGAATGACAGTGGCGCTGGT
  GGTCTTGGTCAGAGGGAGCGGAAGAAGAAGAAGAAGCGGCCCCCTGGGTACTACAGTTATCTGAAAGATG
  GTAGTGAAGAGGGTGCTTCCCCGGCCGCCCTTGTCAACGGCCATGCCACGTCAGTGGGCACGAACAGCGA
  GGGTGTGGAGGATCCTGAGTTCATGGTGGACATGCTTCCTTCAGTCATGCCCAGGACTTGTGACAGCCCT
  CAGAACCCCATGGACTTAATCAGTGACCCTGTCCCTGATAGTCCTTTCCCCAGGACACTAGGTGGTGATG
  CCAGGACTGCAGGACTGCCTGAGGGCTGCCGTGAAACTGACTTTGAGCAGCCCTGCCTCCCTACAGACAA
  CCTGCTAAGGACAGCTGTGACGCAGCCCAACGCTGGCGCCGACACTACTGAGAACCTCGCAGTTGCTAAT
  GGAAAAATACTTGAATCCTTGGGCGAGGGCACTGCTGCTGCCAATGGGGTAGAGCTGCACACTGATGAGA
  GTGCAGACTTGGACCCTGCGAAGCCTGAGAGCCAGTCACCTCCTGCCGAAAGTGCGCTCTCTGTCTCTGG
  CGCCATTTCCATTAGCCAACCTGCAAAGTCCTGGGCTAGCCTCTTTCATGATTCTAAGCCTTCTTCCTCC
  TCACCCGTGGCGTATGTGGAAACTAAGTGTTCCCCCCCTGTCCCGTCCCCCCTGGCCTCTGAAAAACAGA
  TGGAAGTCAAAGAAGGGCTTGTTCCCGTGTCAGAGGACCCCGTAGCCATAAAGATTGCAGAGTTATTGGA
  GACTGTAACCCTAGTACATAAGCCAGTGTCATTGCAACCCCGTGGGCTGATCAATAAAGGAAACTGGTGC
  TACATTAATGCCACCCTGCAGGCATTGGTGGCTTGCCCTCCGATGTATCACCTGATGAAGTTCATTCCTC
  TGTACTCAAAAGTGCAAAGGCCGTGCACGTCCACGCCCATGATAGATAGCTTTGTTCGGCTCATGAACGA
  GTTTACTAATATGCCAGTACCTCCCAAACCCCGCCAAGCTCTTGGGGATAAAATCGTGAGAGATATCCGC
  CCAGGAGCTGCCTTTGAACCCACATACATTTATCGACTCCTGACAGTCATCAAGTCGAGCCTGTCTGAAA
  AGGGCCGACAGGAGGACGCCGAGGAGTACCTAGGCTTCATCCTCAATGGACTCCACGAGGAGATGCTGAG
  CCTGAAGAAGCTTCTCTCACCCACGCACGAAAAGCACTCTGTTTCTAATGGGCCCGGAAGCCACTTGATA
  GAGGATGAGGAGCTGGAAGACACAGGCGAGGGCAGTGAGGACGAGTGGGAGCAAGTGGGTCCCAAGAACA
  AGACGTCTGTCACCCGGCAGGCGGATTTCGTTCAGACGCCTATCACTGGCATTTTCGGTGGACACATCAG
  GTCTGTGGTTTACCAGCAGAGTTCCAAAGAATCTGCCACGCTGCAGCCATTTTTCACACTGCAGTTGGAC
  ATCCAGTCCGACAAGATTCGCACAGTCCAGGATGCCTTGGAAAGCTTGGTGGCAAGAGAGTCTGTCCAGG
  GCTACACCACCAAGACCAAGCAGGAGGTTGAAGTCAGCCGTAGAGTGACTCTGGAGAAGCTTCCCCCTGT
  CCTTGTGCTCCACCTGAAGCGCTTCGTCTATGAGAAGACGGGCGGATGCCAGAAGCTCGTCAAGAACATC
  GAGTACCCTGTGGACTTGGAGATCAGCAGAGAACTGCTTTCTCCAGGCGTCAAAAATAAAAACTTTAAAT
  GCCACAGAACCTATAGGCTGTTCGCAGTGGTCTACCATCATGGCAACAGTGCTACAGGTGGCCACTACAC
  CACGGACGTCTTCCAGATTGGGCTTAACGGCTGGCTGCGAATTGATGACCAGACGGTCAAGGTTATCAAC
  CAGTACCAGGTGGTGAGACCAAGTGCTGACCGCACCGCCTACCTCCTGTATTACCGCCGTGTGGACCTGC
  TG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201548 representing NM_001034146
  Red=Cloning site Green=Tags(s)
  
  MALHNPQYIFGDFSPDEFNQFFVTPRSSVELPPYSGTQCGIQAEEELLDGQEHQRIEFGVDEVIEPSDGL
  QRAPSYSISSTLNPQAPEFILGCPTSKKTPDDIEKDETYSSIDQYPASALALESSSNAEAETLENDSGAG
  GLGQRERKKKKKRPPGYYSYLKDGSEEGASPAALVNGHATSVGTNSEGVEDPEFMVDMLPSVMPRTCDSP
  QNPMDLISDPVPDSPFPRTLGGDARTAGLPEGCRETDFEQPCLPTDNLLRTAVTQPNAGADTTENLAVAN
  GKILESLGEGTAAANGVELHTDESADLDPAKPESQSPPAESALSVSGAISISQPAKSWASLFHDSKPSSS
  SPVAYVETKCSPPVPSPLASEKQMEVKEGLVPVSEDPVAIKIAELLETVTLVHKPVSLQPRGLINKGNWC
  YINATLQALVACPPMYHLMKFIPLYSKVQRPCTSTPMIDSFVRLMNEFTNMPVPPKPRQALGDKIVRDIR
  PGAAFEPTYIYRLLTVIKSSLSEKGRQEDAEEYLGFILNGLHEEMLSLKKLLSPTHEKHSVSNGPGSHLI
  EDEELEDTGEGSEDEWEQVGPKNKTSVTRQADFVQTPITGIFGGHIRSVVYQQSSKESATLQPFFTLQLD
  IQSDKIRTVQDALESLVARESVQGYTTKTKQEVEVSRRVTLEKLPPVLVLHLKRFVYEKTGGCQKLVKNI
  EYPVDLEISRELLSPGVKNKNFKCHRTYRLFAVVYHHGNSATGGHYTTDVFQIGLNGWLRIDDQTVKVIN
  QYQVVRPSADRTAYLLYYRRVDLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001034146
• ORF Size: 2382 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034146.1, NP_001029318.1
• RefSeq Size: 3287 bp
• RefSeq ORF: 2385 bp
• Locus ID: 307905
• UniProt ID: Q3KR59
• Cytogenetics: 19q12
• MW: 87.3 kDa
• Gene Summary: Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation via deubiquitinating IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage. Deubiquitinates TBX21 leading to its stabilization.[UniProtKB/Swiss-Prot Function]