Capn10 (NM_031673) Rat Tagged ORF Clone

CAT#: RR201453

Capn10 (Myc-DDK-tagged ORF) - Rat calpain 10 (Capn10), (10 ug)

Product Images

Circular map for RR201453

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Capn10
• Synonyms: MGC93346
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201453 representing NM_031673
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGCGGTCCGGGCCGAGACGCGGGCGCGGGAGCTCTTCCGGGACGCCGCATTCCCCGCCTCGGACT
  CCTCGCTCTTTTACAACTTGTCCACGCCTCTGGCCCAGTTTCGGGAGGACATCACTTGGAGACGGCCCCA
  GGACATTTGTGCCACACCTCAACTGTTTCCAGATAACCCATGGGAAGGACAGGTGAAGCAAGGGCTGCTG
  GGGGATTGCTGGTTCCTGTGTGCCTGTGCCGCCCTTCAGAAGAGTCGACACCTCCTGGACCAGGTCTTCC
  CTCCAGGACAGCCAGGCTGGTCTGACCAGGAATACCAAGGCTTCTTCACCTGTCGGATTTGGCAGTTTGG
  ACACTGGGAGGAAGTGACCATAGATGACCGTCTGCCTTGTCTTGCCGGGAGACTCTGCTTTTCTCGATGC
  CAGAGAGAGGATGTGTTCTGGCTTCCCTTACTGGAAAAGGCCTACGCTAAGGTTCATGGATCCTATGAGC
  ACCTGTGGGCAGGGCAGGTGGCAGATGCCCTGGTGGATCTCACTGGCAGCCTGGCAGAGAGGTGGAGCCT
  GAAGGACATAAGGAAAGCCAGCGGCCAGCAGGACAGACCCAGTGGTGGAGAGCACAGAGCCTGTCAGCAG
  CTACTCCGTCTCAAGGACCAGTGTCTGCTCAGCTGCTCTGTGCTGAGCCCGAGAGCAGGTGCTAGGGAGC
  TCGGGGAGTTCCATGCCTTCATTATCTCAGATCTGCAGGAGCTCAGGAGTCAGACTGGTCAGGGTATCCT
  CCTGCTGAGGATTCACAACCCTTGGGGCCGGCGTTGTTGGCAGGGCCTCTGGAGAGAAGGCGGTGAAGGG
  TGGAACCAGGTAGAGCCAGCTAAGGAGTCTGAGCTGCTGGCACAACTCCAGGAAGGAGAGTTCTGGGTAG
  AGGAAGAGGAGTTCCTCAGGGAGTTTGATGAGGTCACCATCGGCTACCCAGTCACAGAGGCCGGCCACCT
  GCAAAGTCTCTACACAGAGAAGGTGCTGTGCCATACGCGGGCACTGCCTGGTGCCTGGGTGACAGGGCAA
  TCAGCTGGAGGCTGCCGGAACAACAGTTGCTTTCCCTGCAACCCCAAGTTCTGGTTACGGCTCTTGGAAC
  CCAGTGAGGTGTGTGTGGCTGTCCTTCAGAGACCCCGGAGGCGCTTAGTGGGCCAGACCCGGGCACTGGC
  GGGCGCCAGCCCTGCACCAGTGAACCTCCCAGGCAAAGACTACCAGGCTGTGGGCCTGCACATCTGGAAG
  GTAGAGAAACGGAAGATCAGCCTGCCCAGAGTCCTGTCTGCACCCCCGGTGGCTGGCACTGCATGCCATG
  CGTATGATCGTGAGATCCACTTGCGTTGTGAGCTCTCACCAGGCTACTACCTGGCTGTCCCTAGCACCTT
  TTTGAAGGATGTGCCAGGGCAGTTCCTGCTCCGAGTCTTCTCCACCGGGAAAATCTCCCTCAGTGCCGTC
  AGGCTGGCCACCAAGGGTGCATCACCTGGAGCAGCCCTGCCTGCAGGCGAGTGGGAGACTGTGCAGTTGC
  AGGGCAGCTGGAGAGCTGGCCAGACAGCCGGGGGCAGTAGAAACTTTGCCTCTTACCCCTGCAATCCCTG
  CCTCCCCTTCTCTGTTCCTGAGGGTGCTGGTCCCCGCTACATCCGTATCACCCTACAGCAACACTGCCGG
  CTCAGTGACAGCCAGCTCCACCCCATTGGTTTCCATGTCTTTCAGGTTCCAGCAGACGGTGAAAAACAGG
  ATGCGTGTTCCCTGCTGCTCCAGGAGCCACTGCTAAGCTGTGTACCACATTGCTATGCCCAGGAAGTAAG
  CCGCCTCTGCCTCCTTTCTGCGGGGAACTACAGGATTGTGCCCTCCACCTACCTGCCAGATACAGAGGGT
  ACCTTCACAGTGACCATAGCAACCAGAATAGACAGACAGTCCATTCACAGCCAGGAGATGCTGGGCCAGC
  TACTCCAGGAGGTCTCCTTCATGGCAGTGATGAAAGCCTACTCCAGGAGGTCTCCTTCATGGCAGTGATG
  AAAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201453 representing NM_031673
  Red=Cloning site Green=Tags(s)
  
  MRAVRAETRARELFRDAAFPASDSSLFYNLSTPLAQFREDITWRRPQDICATPQLFPDNPWEGQVKQGLL
  GDCWFLCACAALQKSRHLLDQVFPPGQPGWSDQEYQGFFTCRIWQFGHWEEVTIDDRLPCLAGRLCFSRC
  QREDVFWLPLLEKAYAKVHGSYEHLWAGQVADALVDLTGSLAERWSLKDIRKASGQQDRPSGGEHRACQQ
  LLRLKDQCLLSCSVLSPRAGARELGEFHAFIISDLQELRSQTGQGILLLRIHNPWGRRCWQGLWREGGEG
  WNQVEPAKESELLAQLQEGEFWVEEEEFLREFDEVTIGYPVTEAGHLQSLYTEKVLCHTRALPGAWVTGQ
  SAGGCRNNSCFPCNPKFWLRLLEPSEVCVAVLQRPRRRLVGQTRALAGASPAPVNLPGKDYQAVGLHIWK
  VEKRKISLPRVLSAPPVAGTACHAYDREIHLRCELSPGYYLAVPSTFLKDVPGQFLLRVFSTGKISLSAV
  RLATKGASPGAALPAGEWETVQLQGSWRAGQTAGGSRNFASYPCNPCLPFSVPEGAGPRYIRITLQQHCR
  LSDSQLHPIGFHVFQVPADGEKQDACSLLLQEPLLSCVPHCYAQEVSRLCLLSAGNYRIVPSTYLPDTEG
  TFTVTIATRIDRQSIHSQEMLGQLLQEVSFMAVMKAYSRRSPSWQ**K
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031673
• ORF Size: 1998 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 2561 bp
• RefSeq ORF: 2001 bp
• Locus ID: 63834
• UniProt ID: Q9ES66
• Cytogenetics: 9q36
• MW: 74.5 kDa
• Gene Summary: large subunit of calpain, a calcium-dependent cysteine protease; human homolog has been associated with type II diabetes mellitus [RGD, Feb 2006]