Bmp2 (NM_017178) Rat Tagged ORF Clone

CAT#: RR201404

Bmp2 (Myc-DDK-tagged ORF) - Rat bone morphogenetic protein 2 (Bmp2), (10 ug)

Product Images

Circular map for RR201404

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bmp2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201404 representing NM_017178
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGCCGGGACCCGCTGTCTTCTAGTGTTGCTGCTTCCCCAGGTCCTCCTGGGCGGCGCGGCCGGCC
  TCATTCCGGAGCTGGGCCGCAAGAAGTTCGCCGGGGCATCGCGCCCCTTATCCCGGCCTTCGGAGGACGT
  CCTCAGCGAGTTTGAGTTGAGGCTGCTCAGCATGTTTGGCCTGAAGCAGAGACCCACCCCCAGCAAGGAC
  GTCGTGGTGCCCCCCTATATGCTCGACCTGTACCGCCGGCACTCGGGCCAGCCAGGAGCGCTCGCCCCAG
  ACCACCGGCTGGAGAGGGCAGCCAGCCGCGCCAACACCGTGCTCAGCTTCCATCACGAAGAAGCCATCGA
  GGAACTTTCAGAAATGAGTGGGAAAACTTCCCGACGCTTCTTCTTCAATTTAAGTTCTGTCCCTACTGAT
  GAGTTTCTCACATCTGCAGAGCTCCAGATTTTTCGGGAACAAATGCAGGAAGCTTTGGGAAATAGTAGTT
  TCCAGCACCGAATTAATATTTATGAAATTATAAAGCCTGCCACAGCCAGCTCAAAATTTCCTGTGACCAG
  ACTATTGGACACCAGGTTAGTGACTCAGAACACAAGTCAGTGGGAGAGCTTTGATGTCACCCCGGCTGTG
  ATGCGATGGACAGCACAGGGACACACCAACCATGGGTTTGTGGTGGAAGTGGCCCACTTAGAGGAGAAGC
  CAGGTGTCTCCAAGAGACATGTGAGGATTAGCAGGTCTTTGCACCAAGATGAACACAGCTGGTCTCAGGT
  AAGACCACTGCTAGTGACTTTTGGCCACGACGGTAAAGGACATCCACTCCACAAACGAGAAAAGCGTCAA
  GCCAAACACAAACAGCGGAAGCGTCTTAAGTCCAGCTGCAAAAGGCACCCTTTGTATGTGGACTTCAGTG
  ATGTGGGGTGGAATGACTGGATCGTGGCCCCTCCAGGCTATCATGCCTTTTACTGCCATGGGGAATGTCC
  TTTTCCCCTGGCTGATCACCTGAACTCCACCAACCATGCCATAGTGCAGACTCTGGTAAACTCTGTGAAT
  TCCAAAATCCCTAAGGCATGCTGTGTCCCCACTGAGCTTAGCGCAATCTCCATGTTGTACCTAGATGAAA
  ACGAAAAGGTTGTGCTAAAAAACTATCAGGACATGGTTGTGGAGGGTTGCGGGTGTCGCACGAAAAGGTT
  GTGCTAAAAAACTATCAGGACATGGTTGTGGAGGGTTGCGGGTGTCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201404 representing NM_017178
  Red=Cloning site Green=Tags(s)
  
  MVAGTRCLLVLLLPQVLLGGAAGLIPELGRKKFAGASRPLSRPSEDVLSEFELRLLSMFGLKQRPTPSKD
  VVVPPYMLDLYRRHSGQPGALAPDHRLERAASRANTVLSFHHEEAIEELSEMSGKTSRRFFFNLSSVPTD
  EFLTSAELQIFREQMQEALGNSSFQHRINIYEIIKPATASSKFPVTRLLDTRLVTQNTSQWESFDVTPAV
  MRWTAQGHTNHGFVVEVAHLEEKPGVSKRHVRISRSLHQDEHSWSQVRPLLVTFGHDGKGHPLHKREKRQ
  AKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVN
  SKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCRTKRLC*KTIRTWLWRVAGV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017178
• ORF Size: 1179 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 1275 bp
• RefSeq ORF: 1182 bp
• Locus ID: 29373
• UniProt ID: P49001
• Cytogenetics: 3q36
• MW: 44.4 kDa
• Gene Summary: involved in cellular signaling during limb development; induces bone formation [RGD, Feb 2006]