Havcr1 (NM_173149) Rat Tagged ORF Clone

CAT#: RR200917

Havcr1 (Myc-DDK-tagged ORF) - Rat hepatitis A virus cellular receptor 1 (Havcr1), (10 ug)

Product Images

Circular map for RR200917

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Havcr1
• Synonyms: KIM-1; Kim1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200917 representing NM_173149
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCAACTTCAAGTCTTCATTTCAGGCCTCCTGCTGCTTCTTCCAGGCTCTGTAGATTCTTATGAAG
  TAGTGAAGGGGGTGGTGGGTCACCCTGTCACAATTCCATGTACTTACTCAACACGTGGAGGAATCACAAC
  GACATGTTGGGGCCGGGGGCAATGCCCATATTCTAGTTGTCAAAATATACTTATTTGGACCAATGGATAC
  CAAGTCACCTATCGGAGCAGCGGTCGATACAACATAAAGGGGCGTATTTCAGAAGGAGACGTATCCTTGA
  CAATAGAGAACTCTGTTGATAGTGATAGTGGTCTGTATTGTTGCCGAGTGGAGATTCCTGGATGGTTCAA
  CGATCAGAAAATGACCTTTTCATTGGAAGTTAAACCAGAAATTCCCACAAGTCCTCCAACAAGACCCACA
  ACTACAAGACCCACAACCACAAGGCCCACAACTATTTCAACAAGATCCACACATGTACCAACATCAACCA
  GAGTCTCCACCTCTACTCCAACACCAGAACAAACACAGACTCACAAACCAGAAATCACTACATTTTATGC
  CCATGAGACAACTGCTGAGGTGACAGAAACTCCATCATATACTCCTGCAGACTGGAATGGCACTGTGACA
  TCCTCAGAGGAGGCCTGGAATAATCACACTGTAAGAATCCCTTTGAGGAAGCCGCAGAGAAACCCGACTA
  AGGGCTTCTATGTTGGCATGTCCGTTGCAGCCCTGCTGCTGCTGCTGCTTGCGAGCACCGTGGTTGTCAC
  CAGGTACATCATTATAAGAAAGAAGATGGGCTCTCTGAGCTTTGTTGCCTTCCATGTCTCTAAGAGTAGA
  GCTTTGCAGAACGCAGCGATTGTGCATCCCCGAGCTGAAGACAACATCTACATTATTGAAGATAGATCTC
  GAGGTGCAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200917 representing NM_173149
  Red=Cloning site Green=Tags(s)
  
  MVQLQVFISGLLLLLPGSVDSYEVVKGVVGHPVTIPCTYSTRGGITTTCWGRGQCPYSSCQNILIWTNGY
  QVTYRSSGRYNIKGRISEGDVSLTIENSVDSDSGLYCCRVEIPGWFNDQKMTFSLEVKPEIPTSPPTRPT
  TTRPTTTRPTTISTRSTHVPTSTRVSTSTPTPEQTQTHKPEITTFYAHETTAEVTETPSYTPADWNGTVT
  SSEEAWNNHTVRIPLRKPQRNPTKGFYVGMSVAALLLLLLASTVVVTRYIIIRKKMGSLSFVAFHVSKSR
  ALQNAAIVHPRAEDNIYIIEDRSRGAE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173149
• ORF Size: 921 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173149.2, NP_775172.1
• RefSeq Size: 2538 bp
• RefSeq ORF: 924 bp
• Locus ID: 286934
• UniProt ID: O54947
• Cytogenetics: 10q21
• MW: 34 kDa
• Gene Summary: may be a member of the mucosal addressin cell adhesion molecule family; may be involved in restoration of the morphological integrity and function to postischemic kidney [RGD, Feb 2006]