Cd47 (NM_019195) Rat Tagged ORF Clone

CAT#: RR200816

Cd47 (Myc-DDK-tagged ORF) - Rat Cd47 molecule (Cd47), (10 ug)

Product Images

Circular map for RR200816

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cd47
• Synonyms: Itgp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200816 representing NM_019195
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCCCTTGGCGGCGGCGCTGTTGCTGGGCTCCTGCTGCTGCGGTTCAGCTCAACTCCTGCTTAGTA
  AAGTCAAATCTGTAGAGTTCACGTCATGCAATGACACTGTGGTCATCCCTTGCAAAGTCCTTAATGTGGA
  AGCCCAAAGCACCGATGAAATGTTTGTGAAGTGGAAGTTGAACAAATCGTATATTTTCATCTATGATGGA
  AATAAAAATAGCACTACTAGAGAACAAAACTTTACCAGTGCAAAAATCTCGGTCTCAGACTTGCTCAAAG
  GCATTGCCTCTTTGACAATGGATACCCATGAGGCTGTGGTGGGAAACTACACCTGTGAAGTGACTGAGCT
  TTCCAGAGAAGGCAAAACAGTTATAGAGCTGAAAAACCGCCCAGTTTCGTGGTTTTCTACAAATGAAAAG
  ATCCTCATTGTTATTTTCCCAATTTTGGCTATACTCCTGTTCTGGGGAAAGTTTGGTATATTAACACTCA
  AATACAAGTCCAGCCATACGAATAAGAGAATCATTCTGCTGCTGGTTGCCGGGCTGGCGCTCACACTCAT
  CGTGGTTGTTGGCGCCATCCTTTTCATCCCGGGAGAGAAGCCCGTGAAGAACGCTTCTGGTCTTGGCCTC
  ATTGTAATCTCCACAGGAATATTAATACTGCTTCAGTACAATGTGTTTATGACAGCTTTTGGAATGACCT
  CTTTCACCATTGCCATATTGATCACTCAAGTGCTGGGCTATGTCCTTGCTGTGGTCGGGATGTGTCTCTG
  CATCATGGCTTGTGAACCAGTGCACGGCCCCCTTTTGATTTCAGGTTTGGGAATCATAGCTCTAGCAGAA
  TTACTTGGACTAGTTTATATGAAGTTTGTCGCTTCCAACCAGCGGACTATACAACCTCCTAGGAATAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200816 representing NM_019195
  Red=Cloning site Green=Tags(s)
  
  MWPLAAALLLGSCCCGSAQLLLSKVKSVEFTSCNDTVVIPCKVLNVEAQSTDEMFVKWKLNKSYIFIYDG
  NKNSTTREQNFTSAKISVSDLLKGIASLTMDTHEAVVGNYTCEVTELSREGKTVIELKNRPVSWFSTNEK
  ILIVIFPILAILLFWGKFGILTLKYKSSHTNKRIILLLVAGLALTLIVVVGAILFIPGEKPVKNASGLGL
  IVISTGILILLQYNVFMTAFGMTSFTIAILITQVLGYVLAVVGMCLCIMACEPVHGPLLISGLGIIALAE
  LLGLVYMKFVASNQRTIQPPRNN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019195
• ORF Size: 909 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019195.2, NP_062068.1
• RefSeq Size: 1952 bp
• RefSeq ORF: 912 bp
• Locus ID: 29364
• UniProt ID: P97829
• Cytogenetics: 11q21
• MW: 33.4 kDa
• Gene Summary: protein that may be involved in regulating renal cell growth; overexpression is associated with Fe-NTA-induced renal damage and tumor progression and metastasis [RGD, Feb 2006]