Nos3 (NM_021838) Rat Tagged ORF Clone

CAT#: RR200806

Nos3 (Myc-DDK-tagged ORF) - Rat nitric oxide synthase 3, endothelial cell (Nos3), (10 ug)

Product Images

Circular map for RR200806

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nos3
• Synonyms: eNos
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200806 representing NM_021838
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAACTTGAAGAGTGTGGGCCAGGAGCCTGGGCCACCCTGTGGCCTAGGGCTCGGGCTGGGCCTAG
  GGCTATGCGGCAAGCAGGGCCCAGCCTCACCGGCACCAGAGCCTAGCCAGGCACCAGTACCCCCGTCCCC
  AACCCGACCAGCACCAGACCACAGCCCCCCGTTAACCCGGCCCCCAGACGGACCCAAGTTTCCTCGAGTA
  AAGAACTGGGAAGTGGGCAGCATCACCTACGATACCCTCAGTGCACAGGCTCAGCAGGATGGGCCCTGTA
  CCCCAAGACGCTGCTTGGGATCCCTGGTATTTCCAAGGAAGTTACAGAGCCGGCCCACCCAGGGCCCTTC
  ACCCACTGAGCAGCTATTGGGTCAAGCCCGGGACTTCATCAATCAGTACTATAACTCGATCAAAAGGAGT
  GGTTCCCAGGCTCATGAGCAGCGGCTTCAGGAAGTGGAAGCTGAGGTGGTGGCCACGGGCACCTACCAGC
  TCCGGGAGAGTGAGCTGGTGTTTGGGGCCAAACAGGCCTGGCGCAACGCTCCCCGCTGTGTGGGGCGGAT
  CCAGTGGGGGAAACTGCAGGTATTTGATGCTCGGGACTGCAGGACAGCACAGGAAATGTTCACCTACATC
  TGTAACCACATTAAGTATGCAACAAACCGAGGCAATCTTCGTTCAGCCATCACGGTGTTCCCCCAGCGCT
  ACGCTGGCCGGGGAGACTTCCGGATCTGGAACAGCCAGCTGGTGCGCTACGCGGGCTATAGGCAGCAGGA
  CGGCTCTGTGCGAGGGGACCCTGCCAACGTGGAGATCACTGAGCTCTGTATTCAACATGGCTGGACCCCA
  GGAAATGGCCGCTTTGATGTGCTGCCCCTGCTACTCCAGGCTCCCGATGAGCCCCCAGAACTCTTCACTC
  TGCCCCCAGAGCTGGTCCTCGAGGTGCCTCTGGAGCACCCCACGCTAGAGTGGTTTGCTGCCCTTGGCCT
  GCGCTGGTATGCCCTCCCAGCTGTGTCCAATATGCTGCTAGAAATCGGGGGCCTGGAGTTTCCGGCTGCC
  CCTTTCAGCGGCTGGTACATGAGTTCAGAGATTGGCATGAGGGACCTGTGTGACCCTCACCGATACAACA
  TACTTGAGGATGTGGCTGTCTGCATGGATCTAGACACCCGGACAACCTCATCACTGTGGAAAGACAAGGC
  AGCAGTGGAAATTAACGTGGCTGTGCTGTACAGTTACCAGCTGGCCAAAGTGACCATTGTGGACCACCAT
  GCCGCCACAGCCTCCTTCATGAAGCACTTGGAAAATGAGCAGAAGGCCAGAGGGGGCTGCCCCGCTGACT
  GGGCCTGGATCGTGCCCCCCATCTCAGGCAGCCTCACCCCTGTCTTCCATCAAGAGATGGTCAACTATTT
  CCTGTCCCCTGCCTTCCGCTACCAGCCTGACCCCTGGAAAGGAAGTGCAGCAAAAGGCACAGGCATCACC
  AGGAAGAAGACTTTTAAGGAAGTAGCCAATGCAGTGAAGATCTCTGCCTCACTCATGGGCACGGTGATGG
  CGAAGCGTGTGAAGGCGACTATCCTGTATGGCTCTGAGACTGGCCGCGCCCAGAGCTACGCACAGCAGCT
  GGGGAGGCTCTTTCGGAAGGCGTTTGACCCCCGGGTCCTGTGCATGGATGAATACGATGTGGTATCCCTA
  GAGCATGAGGCCTTGGTATTGGTGGTGACCAGCACATTTGGCAATGGGGATCCCCCGGAGAATGGAGAGA
  GCTTTGCAGCAGCGCTGATGGAAATGTCGGGCCCCTACAACAGCTCCCCTCGGCCTGAGCAGCACAAGAG
  TTACAAAATCCGATTCAACAGTGTCTCCTGCTCGGACCCACTGGTATCCTCTTGGCGGCGCAAAAGGAAG
  GAATCCAGTAACACAGACAGTGCAGGGGCCCTGGGCACCCTCAGGTTCTGTGTGTTTGGGCTGGGCTCCA
  GAGCATACCCGCACTTCTGTGCCTTTGCTCGAGCGGTGGACACAAGGCTGGAGGAGCTGGGCGGGGAGCG
  ACTGTTGCAGCTGGGCCAGGGTGATGAGCTCTGCGGCCAGGAGGAGGCTTTCCGAGGCTGGGCCCAGGCA
  GCCTTCCAGGCTGCCTGTGAAACTTTCTGTGTGGGAGAAGATGCCAAGGCTGCTGCCCGAGATATCTTCA
  GTCCCAAGCGCAGCTGGAAACGCCAGAGGTACCGGCTGAGTACCCAAGCTGAGAGCCTGCAATTACTGCC
  AGGGCTGACTCACGTGCACAGACGGAAGATGTTCCAGGCTACAATTCTTTCTGTGGAGAACCTACAGAGC
  AGCAAATCCACCCGAGCCACAATCCTGGTGCGTCTGGACACTGGAAGCCAGGAGGGACTGCAGTACCAGC
  CAGGGGACCACATAGGTGTGTGCCCACCCAACCGGCCTGGCCTAGTGGAGGCGCTGCTGAGCCGAGTGGA
  GGACCCTCCGCCATCCACAGAGCCTGTGGCCGTGGAACAACTGGAAAAAGGCAGCCCTGGTGGCCCTCCC
  CCCGGCTGGGTACGGGACCCCCGGCTTCCCCCATGTACGCTGCGCCAGGCTCTCACTTACTTCCTGGACA
  TCACTTCCCCGCCCAGCCCTCGCCTTCTTCGACTGCTGAGCACCCTGGCAGAGGAGTCCAGCGAACAGCA
  GGAGCTAGAGGCTCTTAGCCAGGACCCCCGGCGCTACGAAGAATGGAAGTGGTTCCGCTGCCCCACACTG
  CTAGAGGTGCTGGAACAATTTCCATCCGTGGCACTGCCTGCCCCGCTGATCCTCACCCAGCTGCCCCTGC
  TCCAGCCCCGGTACTACTCTGTCAGCTCAGCACCCAGCGCCCACCCAGGAGAGATCCACCTCACTGTAGC
  TGTGCTGGCATACAGAACCCAGGATGTGCTGGGCCCTCTGCACTATGGGGTCTGTTCCACATGGATGAGC
  CAACTCAAGGCAGGAGACCCGGTGCCCTGCTTCATCAGGGGGGCTCCCTCCTTCCGGCTGCCACCTGATC
  CTAACTTGCCTTGCATCCTGGTGGGCCCAGGGACTGGTATTGCACCCTTCCGGGGATTCTGGCAAGACCG
  ATTACACGACATTGAGATCAAAGGACTGCAGCCTGCCCCCATGACTTTGGTGTTTGGCTGCCGATGCTCC
  CAACTGGACCATCTCTACCGGGACGAGGTACTGGACGCCCAGCAGCGTGGAGTGTTTGGACAAGTCCTCA
  CCGCCTTTTCCAGGGATCCTGGCAGCCCTAAGACCTATGTGCAAGACCTCCTGAGGACAGAGCTGGCCGC
  GGAGGTTCACCGCGTGCTGTGCCTCGAGCAAGGACACATGTTTGTCTGCGGTGATGTCACTATGGCAACC
  AGCGTCCTGCAAACCGTGCAGCGAATTCTGGCAACAGAGGGCAGCATGGAGCTGGATGAAGCCGGTGACG
  TCATCGGCGTGCTGCGGGATCAGCAACGCTACCACGAGGACATTTTCGGACTCACATTGCGCACCCAGGA
  GGTGACGAGCCGCATCCGCACCCAGAGTTTTTCTTTGCAGGAGCGACAGCTGAGGGGTGCAGTGCCCTGG
  TCCTTTGACCCGCCTACCCAAGAAACACCTGGTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200806 representing NM_021838
  Red=Cloning site Green=Tags(s)
  
  MGNLKSVGQEPGPPCGLGLGLGLGLCGKQGPASPAPEPSQAPVPPSPTRPAPDHSPPLTRPPDGPKFPRV
  KNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQSRPTQGPSPTEQLLGQARDFINQYYNSIKRS
  GSQAHEQRLQEVEAEVVATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRTAQEMFTYI
  CNHIKYATNRGNLRSAITVFPQRYAGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTP
  GNGRFDVLPLLLQAPDEPPELFTLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAA
  PFSGWYMSSEIGMRDLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLYSYQLAKVTIVDHH
  AATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGTGIT
  RKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSL
  EHEALVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSVSCSDPLVSSWRRKRK
  ESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQA
  AFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSTQAESLQLLPGLTHVHRRKMFQATILSVENLQS
  SKSTRATILVRLDTGSQEGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPPSTEPVAVEQLEKGSPGGPP
  PGWVRDPRLPPCTLRQALTYFLDITSPPSPRLLRLLSTLAEESSEQQELEALSQDPRRYEEWKWFRCPTL
  LEVLEQFPSVALPAPLILTQLPLLQPRYYSVSSAPSAHPGEIHLTVAVLAYRTQDVLGPLHYGVCSTWMS
  QLKAGDPVPCFIRGAPSFRLPPDPNLPCILVGPGTGIAPFRGFWQDRLHDIEIKGLQPAPMTLVFGCRCS
  QLDHLYRDEVLDAQQRGVFGQVLTAFSRDPGSPKTYVQDLLRTELAAEVHRVLCLEQGHMFVCGDVTMAT
  SVLQTVQRILATEGSMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPW
  SFDPPTQETPGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021838
• ORF Size: 3606 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021838.2, NP_068610.1
• RefSeq Size: 3953 bp
• RefSeq ORF: 3609 bp
• Locus ID: 24600
• UniProt ID: Q62600
• Cytogenetics: 4q11
• MW: 133.2 kDa
• Gene Summary: enzyme that synthesizes Nitric oxide from L-arginine [RGD, Feb 2006]