Tnfrsf8 (NM_019135) Rat Tagged ORF Clone

CAT#: RR200681

Tnfrsf8 (Myc-DDK-tagged ORF) - Rat tumor necrosis factor receptor superfamily, member 8 (Tnfrsf8), (10 ug)

Product Images

Circular map for RR200681

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tnfrsf8
• Synonyms: Cd30
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200681 representing NM_019135
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCATCCTACTCAAGGCAGCCGGGTTGCTGTTCCTGGGGATGCTTCAAGCCTTCCCAAAGGATCGAC
  CACTGGACACTACCTGTACCGGAGACCTCAGCTACTACCCCGGGGAGGCTGCCAGGAACTGCTGTTACCA
  GTGCCCCTCAGGGTTGTCTCCTACACAGCCATGCCCACAGGGCCCTGCCACTGCCAGAAACAGTGTGATC
  CTGACTACTACATCAAACGAAGACGGGAAGTGCACAGCCTGCGTGACCTGTTTGCCAGGCCTCGTGGAGA
  AGGCTCCATGTTCTGGGAACTCTCCTCGAATCTGTGAGTGTCAGCCCGGCATGTACTGCTCCACACCAGC
  TGTCAATTCCTGTGCCCGATGTTCTGAAAAGACGGTTGTCAAGTTCCCAGACACAGCAGAAAAGAACACT
  ATCTGTGAGCTGCCCTCCCCAGGATCTGGCCCCAATGGCTCCAATCCAGACGACTGCAAAACACTTACTA
  GCCATACCACTCCTCAGGCCATACCTACTCTAGAATCTCCAGCCAATGACAGTGTGAGGAGCTTGCTGCC
  AAAACAGGTCACTGACTTTGTGAACGAAGGTGCTACTAAGCTGGTGAAGGTTCCTGAGTCTTCCTCTTCC
  AAGGCAAGCATGCCTAGTCCAGATCCAGGTAATGCAGAGATGAATATGACCTTGAAGCTTCCACCTCCAG
  GGACAGTCCCTGACATCAGTACTTCAGAAAACAGCATGGAGCCTGCCAGTACCGCCTCCACCCTAAGCCT
  CTTGGTAGATGCCCGGACCAGCAGCAGGATGCAGCCTACATCTCCCTTGTCCACAGGAACACCGTTTCTG
  GATCCAGGGACCATGCTCTTCTGGGTGGCCATGGTGGTGCTGCTGGTTGGCTCTGCCTCCTTCCTCCTGT
  GTTACTGGAAGGCCTGCTGGAGGCGGTTCCAGCGGAAGTTTCACCTGAACTATCTACAGCAGACCTTCCA
  GCCCAAGATGGAGCAGGTAGATTCCTGTCCTACTGAAAAGCTAACTCAGCTGCAGAGAAGTGGGTCAGTG
  ACAGATTCCAGCGCTGGACACACGTTGAGCCCACTGAGCCCTCCAGCTGTAGAGACTGGTGCCAGTGTTG
  GAACCGCCTGCCTGGAGACCCTGCCGCTGCTGGATGACAGCATAACTGGGAACTCTTTTGCTCCCAGAGA
  ACCTCCAGAGCCACGGGTATCCACCGAACACACCAACAACAGGATTGAGAAAATCTACATCATGAAGGCT
  GACACAGCGATTGTGGGCTCAGTAAAAACTGAAGTCCCTGAGGGCCGGGCTCCAGTAGGGTCTACAGAGT
  CTGAGTTGGAAGCTGAACTAGAAGTGGACCATACCCCCCATTACCCGGAGCAGGAGACAGAACCACCTCT
  GGGCAGCTGCACTGAGGTCATGTTCTCCGTGGAGGAAGGAGGAAAAGAAGACCATGAGCCCACAACTGTC
  TCTGAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200681 representing NM_019135
  Red=Cloning site Green=Tags(s)
  
  MSILLKAAGLLFLGMLQAFPKDRPLDTTCTGDLSYYPGEAARNCCYQCPSGLSPTQPCPQGPATARNSVI
  LTTTSNEDGKCTACVTCLPGLVEKAPCSGNSPRICECQPGMYCSTPAVNSCARCSEKTVVKFPDTAEKNT
  ICELPSPGSGPNGSNPDDCKTLTSHTTPQAIPTLESPANDSVRSLLPKQVTDFVNEGATKLVKVPESSSS
  KASMPSPDPGNAEMNMTLKLPPPGTVPDISTSENSMEPASTASTLSLLVDARTSSRMQPTSPLSTGTPFL
  DPGTMLFWVAMVVLLVGSASFLLCYWKACWRRFQRKFHLNYLQQTFQPKMEQVDSCPTEKLTQLQRSGSV
  TDSSAGHTLSPLSPPAVETGASVGTACLETLPLLDDSITGNSFAPREPPEPRVSTEHTNNRIEKIYIMKA
  DTAIVGSVKTEVPEGRAPVGSTESELEAELEVDHTPHYPEQETEPPLGSCTEVMFSVEEGGKEDHEPTTV
  SEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019135
• ORF Size: 1479 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019135.1, NP_062008.1
• RefSeq Size: 3383 bp
• RefSeq ORF: 1482 bp
• Locus ID: 25069
• UniProt ID: P97525
• Cytogenetics: 5q36
• MW: 52.7 kDa
• Gene Summary: Tnfsf8/Cd30l receptor [RGD, Feb 2006]