Ret (NM_001110099) Rat Tagged ORF Clone

CAT#: RR200484

Ret (Myc-DDK-tagged ORF) - Rat ret proto-oncogene (Ret), transcript variant 2, (10 ug)

Product Images

Circular map for RR200484

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ret
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200484 representing NM_001110099
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAAAGCGAGGTCCGGCGCCGCAGGGCTGGGGCTGAAGCTGTTTTTGCTGCTGCCGCTACTGGGAG
  AAGCCCCGCTGGGTCTCTACTTCTCAAGGGATGCTTACTGGGAGAGGCTGTATGTGGACCAGCCAGCTGG
  CACACCTCTGCTCTATGTCCATGCCCTACGGGATGCCCCTGGAGAAGTGCCCAGCTTCCGCCTGGGCCAG
  TATCTCTATGGCGTCTACCGCACGCGTCTGCATGAGAATGACTGGATCCACATCGATTCGGGCACTGGCC
  TCCTCTACCTCAATCAGAGCCTGGACCATAGTTCCTGGGAGCAGCTCAGCATCCGCAATGGCGGCTTCCC
  CTTGCTCACCGTCTTCCTCCAGGTCTTCCTGGGGTCCACAGCCCAGAGAGAGGGAGAGTGTCATTGGCCA
  GGCTGTGCCCGTGTGTACTTCTCCTTCATCAACGACACCTTCCCAAATTGTAGCTCCTTCAAAGCCCGGG
  ATCTCTGCACCCCGGAGACGGGTGTGTCCTTCCGCATCAGGGAGAACAGGCCCCCTGGCACCTTCTACCA
  GTTCCGCATGCTACCTGTGCAGTTCCTTTGTCCTAACATCAGTGTGAAGTACAAACTCTTAGAAGGGGAC
  GGTCTGCCCTTCCGTTGTGACCCCGACTGTCTGGAGGTGAGCACGCGGTGGGCGCTGGATCGGGAGCTTC
  AGGAGAAGTATGTGCTGGAGGCTGAGTGCGCAGTGGCAGGCCCTGGAGCCAACAAGGAGAAGGTGGCCGT
  GTCCTTCCCGGTGACGGTGTATGATGAAGACGACTCCCCGCCCACCTTCTCCGGAGGTGTGGGCACCGCC
  AGTGCTGTGGTGGAGTTTAAGCGGAAGGAGGGCACTGTGGTAGCCACTCTGCAGGTGTTTGATGCAGATG
  TGGTGCCAGCATCTGGGGAGCTGGTGAGGCGGTACACAAGCACACTACTCTCAGGGGATTCCTGGGCCCA
  GCAGACCTTCCGGGTGGAGCACACACCCAACGAGACCTTGGTCCAGTCCAACAACAACTCCGTGCGGGCA
  ACCATGCACAATTACAAGCTGGTTCTCAACAGGAGCCTGTCCATCTCAGAGAGCCGAGTCCTGCAGCTAG
  TAGTCCTGGTCAATGACTCAGACTTCCAGGGGCCTGGGTCAGGTGTTCTCTTCCTCCATTTCAACGTGTC
  TGTGCTGCCTGTCACCCTGAACCTACCCATGGCCTACTCCTTCCCAGTGAATAGGAGAGCCCGCCGTTAT
  GCCCAGATTGGGAAAGTTTGCGTGGAGAACTGCCAGGAGTTCAGCGGTGTCTCCATCCAGTACAAGCTGC
  AGCCCTCCAGCACCAACTGCAGTGCCCTAGGTGTGGTCACCTCAACAGAAGACACCTCAGGGACCCTATA
  TGTAAATGACACGGAGGCCCTGCGGCGACCTGAGTGTACCGAGCTTCAGTACACAGTGGTAGCCACTGAC
  CGGCAGACCCGCAGGCAGACCCAGGCTTCGTTAGTCGTCACAGTGGAGGGGACATACATTGCAGAAGAAG
  TGGGCTGCCCCAAGTCCTGTGCAGTAAACAAGAGGCGACCTGAGTGTGAGGAGTGTGGTGGCCTGGGTTC
  TCCAACTGGCAGATGTGAGTGGCGTCAGGGAGATGGTAAAGGGATCACCAGGAACTTCTCCACCTGTTCT
  CCTAGCACCAGGACCTGTCCTGATGGCCACTGTGATGCTCTGGAGAGCCGGGATATCAACATTTGCCCCC
  AGGACTGTCTCCGTGGCCCCATTGTTGGCGGGCATGAGCGAGGGGAGCGCCAGGGGATTAAAGCCGGCTA
  TGGCATCTGCAACTGTTTCCCTGATGAGAAGAAGTGCTTCTGCGAGCCAGAGGACAGCCAGGGCCCATTG
  TGCGATGAGCTGTGCCGTACGGTCATCACAGCCGCTGTCCTCTTCTCCTTCATAATCTCTGTCCTGCTGT
  CCACCTTCTGCATCCACCGCTACCACAAGCATGCGCACAAGCCACCCATCGCGTCAGCCGAAATGACCTT
  CTGCCGGCCGGCCCAGGGCTTCCCAATCAGCTATTCTTCCTCGGGCACCCGCCGGCCCTCACTGGACTCC
  ATGGAGAACCAGGTCTCTGTGGACTCTTTCAAGATCCCGGAGGATCCGAAGTGGGAATTTCCTCGGAAGA
  ACTTAGTTCTTGGGAAAACCCTGGGAGAAGGCGAGTTTGGAAAAGTAGTCAAGGCCACAGCCTTCCGTCT
  GAAAGGCCGGGCAGGATACACCACAGTGGCTGTGAAAATGCTGAAAGAAAACGCCTCCCAGAGTGAACTA
  CGAGACCTGCTCTCTGAGTTCAACCTTCTGAAACAAGTCAACCATCCACATGTCATCAAGTTGTACGGGG
  CTTGCAGCCAGGATGGGCCACTTCTTCTCATTGTGGAGTATGCAAAGTATGGATCCCTGCGGGGGTTCCT
  GCGGGACAGCCGCAAGATCGGGCCTGCCTATGTGAGCAGTGGAGGCAGCCGCAATTCCAGCTCCCTGGAC
  CACCCAGACGAAAGGGTGCTGACCATGGGCGACCTCATCTCCTTCGCCTGGCAGATCTCGAGGGGTATGC
  AGTACTTGGCTGAAATGAAGCTCGTACATCGAGACTTAGCTGCCAGAAACATCTTGGTGGCAGAGGGACG
  GAAGATGAAGATCTCTGACTTTGGGCTGTCCCGAGATGTTTATGAAGAAGATTCCTATGTGAAGAAAAGC
  AAGGGCCGGATTCCCGTCAAATGGATGGCAATCGAGTCTCTCTTCGATCACATCTATACCACTCAAAGTG
  ATGTGTGGTCCTTTGGAGTGCTGCTATGGGAGATTGTGACCCTGGGAGGCAACCCCTACCCTGGAATTCC
  TCCTGAACGACTCTTCAACCTTCTGAAGACAGGCCACAGGATGGAGAGGCCAGACAACTGCAGCGAGGAA
  ATGTACCGCCTGATGCTGCAGTGCTGGAAGCAGGAGCCGGACAAGAGGCCAGTATTTGCTGACATCAGCA
  AGGATCTGGAGAAGATGATGGTCAAAAGCAGAGACTACTTGGACCTGGCTGCATCCACCCCTTCGGACTC
  ACTGCTCTATGACGATGGGCTCTCGGAAGAGGAGACGCCCCTGGTGGACTGTAACAGTGCTCCCCTCCCG
  CGCTCCCTCCCTTCCACATGGATTGAAAACAAACTCTATGGTAGAATTTCACATGCATTTACTAGATTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200484 representing NM_001110099
  Red=Cloning site Green=Tags(s)
  
  MAKARSGAAGLGLKLFLLLPLLGEAPLGLYFSRDAYWERLYVDQPAGTPLLYVHALRDAPGEVPSFRLGQ
  YLYGVYRTRLHENDWIHIDSGTGLLYLNQSLDHSSWEQLSIRNGGFPLLTVFLQVFLGSTAQREGECHWP
  GCARVYFSFINDTFPNCSSFKARDLCTPETGVSFRIRENRPPGTFYQFRMLPVQFLCPNISVKYKLLEGD
  GLPFRCDPDCLEVSTRWALDRELQEKYVLEAECAVAGPGANKEKVAVSFPVTVYDEDDSPPTFSGGVGTA
  SAVVEFKRKEGTVVATLQVFDADVVPASGELVRRYTSTLLSGDSWAQQTFRVEHTPNETLVQSNNNSVRA
  TMHNYKLVLNRSLSISESRVLQLVVLVNDSDFQGPGSGVLFLHFNVSVLPVTLNLPMAYSFPVNRRARRY
  AQIGKVCVENCQEFSGVSIQYKLQPSSTNCSALGVVTSTEDTSGTLYVNDTEALRRPECTELQYTVVATD
  RQTRRQTQASLVVTVEGTYIAEEVGCPKSCAVNKRRPECEECGGLGSPTGRCEWRQGDGKGITRNFSTCS
  PSTRTCPDGHCDALESRDINICPQDCLRGPIVGGHERGERQGIKAGYGICNCFPDEKKCFCEPEDSQGPL
  CDELCRTVITAAVLFSFIISVLLSTFCIHRYHKHAHKPPIASAEMTFCRPAQGFPISYSSSGTRRPSLDS
  MENQVSVDSFKIPEDPKWEFPRKNLVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASQSEL
  RDLLSEFNLLKQVNHPHVIKLYGACSQDGPLLLIVEYAKYGSLRGFLRDSRKIGPAYVSSGGSRNSSSLD
  HPDERVLTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRDVYEEDSYVKKS
  KGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFNLLKTGHRMERPDNCSEE
  MYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYLDLAASTPSDSLLYDDGLSEEETPLVDCNSAPLP
  RSLPSTWIENKLYGRISHAFTRF
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001110099
• ORF Size: 3219 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001110099.1, NP_001103569.1
• RefSeq Size: 3928 bp
• RefSeq ORF: 3222 bp
• Locus ID: 24716
• Cytogenetics: 4q42
• MW: 119.8 kDa
• Gene Summary: may play a role in excretory system and enteric nervous system development; human homolog is associated with Hirschsprung disease and Multiple Endocrine Neoplasia type 2 [RGD, Feb 2006]