Fgfr2 (NM_001109892) Rat Tagged ORF Clone

CAT#: RR200453

Fgfr2 (Myc-DDK-tagged ORF) - Rat fibroblast growth factor receptor 2 (Fgfr2), transcript variant b, (10 ug)

Product Images

Circular map for RR200453

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fgfr2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200453 representing NM_001109892
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGATTACCGTCCACATGGAGATATGGAACAGGACCAGGGATTGGCACCGTGACCATGGTCAGCTGGG
  GGCGCTTCATCTGCCTGGTCTTGGTCACCATGGCAACCTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGT
  TGAAGATACCACTTTAGAACCAGAAGAGCCACCAACCAAATACCAAATCTCCCAACCAGAAGCGTGCGTG
  GTTGCCCCCGGGGAGTCGCTAGAGTTGCGCTGCATGTTGAAAGATGCCGCCGTGATCAGTTGGACTAAGG
  ATGGGGTGCACTTGGGGCCCAACAATAGGACAGTGCTTATTGGGGAGTACCTCCAGATAAAAGGTGCCAC
  ACCTAGAGACTCCGGCCTCTATGCTTGTGCTGCAGCTAGGACGGTAGACAGTGAAACTTTGTACTTCATG
  GTGAATGTCACAGATGCCATCTCATCTGGAGATGACGAGGACGACACAGATAGCTCCGAAGACTTTGTCA
  GTGAGAACAGGAGCAACCAGAGAGCACCGTACTGGACCAACACCGAAAAGATGGAGAAGCGGCTCCATGC
  TGTCCCTGCCGCCAACACTGTGAAGTTCCGCTGTCCAGCCGGGGGGAATCCAACACCCACAATGAGGTGG
  CTAAAAAACGGGAAGGAGTTTAAGCAGGAGCATCGCATCGGAGGCTATAAGGTACGAAACCAGCACTGGA
  GCCTTATTATGGAAAGTGTGGTCCCATCAGACAAAGGCAATTACACCTGCCTGGTGGAGAATGAATACGG
  GTCCATCAACCACACCTACCACCTTGATGTTGTTGAGCGATCACCACACCGGCCCATCCTCCAAGCTGGA
  CTGCCTGCAAATGCCTCCACGGTGGTCGGAGGGGACGTAGAATTTGTCTGCAAGGTTTATAGTGATGCCC
  AGCCCCATATCCAGTGGATCAAACATGTGGAAAAGAACGGCAGTAAATATGGACCTGATGGGCTGCCCTA
  CCTCAAGGTCCTGAAGGCCGCCGGTGTTAACACCACGGACAAAGAAATTGAGGTTCTCTATATTCGGAAT
  GTAACTTTTGAGGATGCTGGGGAATATACGTGCTTGGCGGGTAATTCTATCGGGATATCCTTTCACTCTG
  CATGGTTGACAGTTCTGCCAGCACCTGTGAGAGAGAAGGAGATCACAGCTTCCCCAGATTACCTGGAGAT
  AGCTATTTACTGCATAGGGGTCTTCTTAATCGCCTGCATGGTGGTGACAGTCATCTTTTGCCGAATGAAG
  ACCACGACCAAGAAGCCAGACTTCAGCAGCCAGCCAGCTGTGCACAAGCTGACCAAGCGCATCCCCCTGC
  GGAGACAGGTAACAGTTTCGGCCGAGTCCAGCTCGTCCATGAACTCCAACACCCCACTGGTGAGGATAAC
  GACACGTCTGTCCTCAACGGCGGACACCCCGATGCTAGCAGGGGTCTCTGAGTACGAGTTGCCAGAGGAT
  CCAAAGTGGGAATTCCCCAGAGATAAGCTGACGCTGGGCAAACCCCTGGGGGAAGGCTGCTTCGGGCAAG
  TAGTCATGGCTGAAGCGGTGGGAATCGATAAGGACAGACCCAAGGAGGCAGTCACCGTGGCGGTGAAGAT
  GTTGAAAGATGACGCCACAGAGAAGGACCTGTCTGACCTGGTGTCAGAGATGGAGATGATGAAGATGATT
  GGTAAACATAAGAACATCATCAACCTCCTGGGGGCCTGCACCCAGGATGGACCCCTCTATGTCATAGTCG
  AATACGCATCGAAAGGCAACCTCCGGGAATACCTCCGGGCCCGGAGGCCACCTGGCATGGAGTACTCCTA
  TGACATTAACCGAGTTCCCGAGGAGCAGATGACCTTCAAGGACTTGGTGTCCTGCACCTACCAGCTGGCG
  AGAGGCATGGAGTACTTGGCTTCCCAAAAATGTATCCATCGAGACTTGGCAGCCAGAAATGTGCTGGTAA
  CAGAAAACAACGTGATGAAGATAGCAGACTTTGGCCTGGCCAGGGATATCAACAACATAGACTATTACAA
  AAAGACCACGAATGGGCGACTTCCAGTCAAGTGGATGGCTCCTGAAGCCCTTTTTGATAGAGTTTACACT
  CATCAGAGTGATGTCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAGGGGGTTCACCCTACC
  CAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCTCAAAGAGGGCCACAGGATGGACAAGCCCACCAACTG
  CACCAATGAACTGTACATGATGATGAGGGACTGCTGGCATGCTGTACCCTCACAGAGGCCCACGTTTAAG
  CAGTTGGTGGAAGACTTGGATCGAATTCTGACTCTCACAACCAATGAGGAATACTTGGACCTCACCCAGC
  CTCTCGAACAGTATTCTCCTAGTTACCCCGACACAAGGAGCTCTTGTTCTTCAGGGGACGATTCTGTGTT
  TTCTCCAGACCCCATGCCTTATGACCCCTGCCTGCCTCAGTATCCACACATAAACGGCAGTGTTAAAACA
  TTCTCCAGACCCCATGCCTTATGACCCCTGCCTGCCTCAGTATCCACACATAAACGGCAGTGTTAAAACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200453 representing NM_001109892
  Red=Cloning site Green=Tags(s)
  
  MGLPSTWRYGTGPGIGTVTMVSWGRFICLVLVTMATLSLARPSFSLVEDTTLEPEEPPTKYQISQPEACV
  VAPGESLELRCMLKDAAVISWTKDGVHLGPNNRTVLIGEYLQIKGATPRDSGLYACAAARTVDSETLYFM
  VNVTDAISSGDDEDDTDSSEDFVSENRSNQRAPYWTNTEKMEKRLHAVPAANTVKFRCPAGGNPTPTMRW
  LKNGKEFKQEHRIGGYKVRNQHWSLIMESVVPSDKGNYTCLVENEYGSINHTYHLDVVERSPHRPILQAG
  LPANASTVVGGDVEFVCKVYSDAQPHIQWIKHVEKNGSKYGPDGLPYLKVLKAAGVNTTDKEIEVLYIRN
  VTFEDAGEYTCLAGNSIGISFHSAWLTVLPAPVREKEITASPDYLEIAIYCIGVFLIACMVVTVIFCRMK
  TTTKKPDFSSQPAVHKLTKRIPLRRQVTVSAESSSSMNSNTPLVRITTRLSSTADTPMLAGVSEYELPED
  PKWEFPRDKLTLGKPLGEGCFGQVVMAEAVGIDKDRPKEAVTVAVKMLKDDATEKDLSDLVSEMEMMKMI
  GKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLRARRPPGMEYSYDINRVPEEQMTFKDLVSCTYQLA
  RGMEYLASQKCIHRDLAARNVLVTENNVMKIADFGLARDINNIDYYKKTTNGRLPVKWMAPEALFDRVYT
  HQSDVWSFGVLMWEIFTLGGSPYPGIPVEELFKLLKEGHRMDKPTNCTNELYMMMRDCWHAVPSQRPTFK
  QLVEDLDRILTLTTNEEYLDLTQPLEQYSPSYPDTRSSCSSGDDSVFSPDPMPYDPCLPQYPHINGSVKT
  FSRPHAL*PLPASVSTHKRQC*N
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001109892
• ORF Size: 2520 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 4651 bp
• RefSeq ORF: 2523 bp
• Locus ID: 25022
• Cytogenetics: 1q37
• MW: 94 kDa
• Gene Summary: may play a role in mesodermal cell differentiation [RGD, Feb 2006]