Slc44a1 (NM_053492) Rat Tagged ORF Clone

CAT#: RR200247

Slc44a1 (Myc-DDK-tagged ORF) - Rat CDW92 antigen (Cdw92), transcript variant 2, (10 ug)

Product Images

Circular map for RR200247

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc44a1
• Synonyms: Cdw92; Ctl1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200247 representing NM_053492
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTGCTGCAGCTCCGCCTCCGCCGCGCAGAGCTCCAAACGAGAATGGAAGCCGTTGGAGGACCGTA
  GCTGCACAGACATACCATGGCTGCTGCTCTTCGTTCTGTTCTGCATTGGGATGGGATTTATTTGTGGCTT
  TTCAGTAGCAACAGGTGCAGCAGCAAGACTAGTGTCAGGATACGACAGCTATGGAAATATCTGTGGGCAG
  AGAAATGCAAAACTGGAAGCAATAGCGAACAGTGGCCTGGACCACACCCACCGGAAGTATGTGTTCTTTT
  TGGACCCATGCAATCTGGACTTGATAAACAGGAAGATCAAGTCTATGGCACTGTGTGTAGCGGCATGTCC
  ACGACAGGAGTTGAAAACCCTGAGTGACGTCCAGAAGTTTGCCGAGATCAATGGTTCAGCACTGTGTAGC
  TACAACATAAAGCCTTCCGAGTACACTCTGACTGCAAAATCCTCTGCCTTTTGCCCCAAACTACCAGTAC
  CAGCCAGTGCACCTATTCCATTCTTCCATCGCTGTGCTCCTGTGAACATTTCCTGCTATGCCAAGTTTGC
  AGAGGCCCTGATCACCTTTGTCAGTGACAATAGTGTCTTACACAGGCTGATTAGTGGAGTAATGACCAGC
  AAAGAAATTATATTGGGACTTTGCTTGTTATCACTAGTTCTGTCCATGATTTTGATGGTGATTATCCGGT
  ATATCTCAAGAGTGCTAGTGTGGATCCTAACGATTCTGGTCATCCTGGGTTCACTAGGTGGCACCGGTGT
  ACTATGGTGGCTATATGCAAAGCAGAGAAGGTCCCCCAAAGAAACAGTTATTCCTGAACAGCTTCAGATC
  GCGGAAGACAATCTGCGGGCGCTCCTCATCTATGCCATTTCTGCTACGGTGTTCACTGTCATTCTCTTCC
  TGATCATGCTGGTTATGCGTAAACGCGTCGCTCTCACCATCGCCTTGTTCCACGTGGCTGGCAAGGTCTT
  CATACACCTGCCTCTGCTAGTCTTCCAGCCCTTCTGGACTTTTTTTGCTCTCGTCTTATTTTGGGCATAC
  TGGATAATGACACTTCTCTTCCTTGGCACTACTGGCAGTGCTGTTCAGAATGAACAAGGTTTCGTGGAGT
  ACAAAATCTCTGGGCCTTTGCAGTACATGTGGTGGTACCACGTGGTCGGCCTGATTTGGATCAGTGAGTT
  CATTCTCGCCTGTCAGCAGATGACTGTGGCAGGAGCTGTGGTAACATACTACTTTACAAGGGATAAACGG
  AATTTGCCTTTCACACCTATTCTGGCATCAGTGAACCGCCTTATTCGTTACCACCTCGGTACTGTGGCCA
  AAGGATCCTTCATTATCACCCTCGTCAAGATCCCCAGGATGATCCTTATGTATATCCACAGTCAGCTCAA
  AGGGAAGGAAAACGCTTGTGCACGATGCATGCTGAAATCCTGCATTTGCTGCCTTTGGTGTCTTGAAAAG
  TGCCTGAGCTACTTAAACCAGAATGCATACACAGCCACAGCCATCAATAGCACCAACTTCTGCACCTCAG
  CAAAGGATGCCTTCGTCATTCTAGTGGAGAATGCTCTGCGAGTGGCTGCCATCAACACAGTGGGGGACTT
  CATGTTATTCCTAGGCAAGGTGCTGATAGTCTGCAGCACGGGCCTGGCTGGGATTATGCTGCTCAACTAC
  CAGCAAGATTACACAGTGTGGGTGCTGCCTCTCATCATCGTCTGCCTCTTTGCTTTCCTAGTCGCTCATT
  GCTTCCTGTCCATTTACGAAATGGTAGTGGATGTGTTGTTTTTGTGTTTTGCCATTGATACAAAATACAA
  TGATGGGAGCCCAGGCCGAGAATTCTATATGGACAAAGTCCTAATGGAGTTTGTGGAAAACAGTAGGAAA
  GCAATGAAAGAAGCTGGTAAGGGAGGCGCCGCGGATGCCCGAGAGCTAAAGCCGATGCTCAGGAAAAGG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200247 representing NM_053492
  Red=Cloning site Green=Tags(s)
  
  MGCCSSASAAQSSKREWKPLEDRSCTDIPWLLLFVLFCIGMGFICGFSVATGAAARLVSGYDSYGNICGQ
  RNAKLEAIANSGLDHTHRKYVFFLDPCNLDLINRKIKSMALCVAACPRQELKTLSDVQKFAEINGSALCS
  YNIKPSEYTLTAKSSAFCPKLPVPASAPIPFFHRCAPVNISCYAKFAEALITFVSDNSVLHRLISGVMTS
  KEIILGLCLLSLVLSMILMVIIRYISRVLVWILTILVILGSLGGTGVLWWLYAKQRRSPKETVIPEQLQI
  AEDNLRALLIYAISATVFTVILFLIMLVMRKRVALTIALFHVAGKVFIHLPLLVFQPFWTFFALVLFWAY
  WIMTLLFLGTTGSAVQNEQGFVEYKISGPLQYMWWYHVVGLIWISEFILACQQMTVAGAVVTYYFTRDKR
  NLPFTPILASVNRLIRYHLGTVAKGSFIITLVKIPRMILMYIHSQLKGKENACARCMLKSCICCLWCLEK
  CLSYLNQNAYTATAINSTNFCTSAKDAFVILVENALRVAAINTVGDFMLFLGKVLIVCSTGLAGIMLLNY
  QQDYTVWVLPLIIVCLFAFLVAHCFLSIYEMVVDVLFLCFAIDTKYNDGSPGREFYMDKVLMEFVENSRK
  AMKEAGKGGAADARELKPMLRKR
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053492
• ORF Size: 1959 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053492.3, NP_445944.2
• RefSeq Size: 2851 bp
• RefSeq ORF: 1962 bp
• Locus ID: 85254
• UniProt ID: Q8VII6
• Cytogenetics: 5q24
• MW: 73.1 kDa
• Gene Summary: may be a choline transporter for a specific subset of cholinergic neurons [RGD, Feb 2006]