Sf1 (NM_058210) Rat Tagged ORF Clone

CAT#: RR200215

Sf1 (Myc-DDK-tagged ORF) - Rat splicing factor 1 (Sf1), transcript variant 2, (10 ug)

Product Images

Circular map for RR200215

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sf1
• Synonyms: Zfp162
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200215 representing NM_058210
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCGGAGCGAACGCCACGCCGCTGGACTTCCCAAGTAAGAAGAGGAAGAGGAGCCGATGGAACC
  AAGACACAATGGAACAGAAGACAGTGATTCCAGGCATGCCTACGGTTATCCCCCCTGGCCTGACTCGGGA
  ACAAGAAAGAGCTTATATAGTGCAACTACAGATAGAAGACCTGACTCGTAAACTGCGCACAGGAGACCTG
  GGCATCCCCCCTAACCCTGAGGACAGGTCCCCTTCCCCTGAGCCAATCTACAACAGCGAGGGGAAGCGGC
  TTAATACTCGAGAATTCCGTACCCGAAAAAAGCTTGAAGAGGAGCGGCATACCCTCATTACGGAGATGGT
  TGCTCTCAACCCAGACTTTAAACCACCTGCAGATTACAAGCCTCCAGCAACACGTGTGAGCGATAAAGTA
  ATGATCCCCCAAGACGAGTATCCAGAAATCAATTTTGTGGGGCTCCTAATTGGGCCCAGAGGGAACACCC
  TGAAGAACATTGAGAAGGAATGCAACGCCAAGATCATGATACGGGGAAAGGGATCAGTAAAAGAAGGGAA
  AGTTGGGCGTAAAGATGGTCAGATGTTGCCAGGAGAAGATGAACCTCTTCATGCTCTAGTCACTGCCAAT
  ACAATGGAGAATGTCAAAAAGGCAGTGGAACAGATCAGAAACATCCTGAAGCAGGGTATTGAAACCCCAG
  AGGACCAGAATGACCTAAGGAAAATGCAGCTTCGAGAGTTAGCTCGCTTGAATGGCACTCTACGGGAAGA
  TGATAACAGGATCTTGAGACCCTGGCAGAGCTCAGAGACACGAAGCATTACCAACACGACTGTGTGTACT
  AAGTGTGGAGGGGCTGGTCACATTGCCTCTGATTGCAAATTTCAGAGGCCTGGTGACCCTCAATCAGCTC
  AGGATAAAGCACGGATGGATAAAGAGTATTTGTCCCTTATGGCTGAGCTAGGGGAAGCTCCTGTCCCTGC
  ATCTGTGGGCTCCACCTCTGGACCTGCCACCACACCCCTGGCCAGTGCACCAAGGCCTGCTGCTCCTGCC
  AGCAACCCACCACCACCGTCTCTCATGTCTACAACTCAGAGTCGCCCACCCTGGATGAATTCTGGTCCTT
  CAGAGAATCGGCCCTACCATGGCATGCATGGAGGTGGTCCTGGTGGGCCTGGAGGTGGCCCCCACAGTTT
  CCCACACCCATTACCCAGCCTGACAGGTGGGCATGGTGGACATCCCATGCAGCACAACCCAAATGGACCA
  CCACCACCTTGGATGCAGCCACCACCACCACCGATGAACCAGGGCCCCCACCCACCTGGGCACCATGGCC
  CTCCTCCAATGGATCAGTACCTGGGAAGTACGCCTGTGGGCTCTGGGGTCTATCGCCTGCATCAAGGAAA
  AGGTATGATGCCGCCGCCGCCTATGGGCATGATGCCGCCGCCTCCGCCACCTCCCAGTGGGCAGCCCCCG
  CCTCCTCCCTCTGGTCCTCTTCCTCCATGGCAGCAGCAGCAGCAGCAGCCTCCACCACCCCCTCCGCCCA
  GCAGCAGTATGGCTTCCAGCACCCCCTTGCCATGGCAGCAAAGATCCCTCCCCGCAGCAGCGATGGCCCG
  AGCCATGAGAGTGAGGACTTTCCGCGCCCATTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200215 representing NM_058210
  Red=Cloning site Green=Tags(s)
  
  MATGANATPLDFPSKKRKRSRWNQDTMEQKTVIPGMPTVIPPGLTREQERAYIVQLQIEDLTRKLRTGDL
  GIPPNPEDRSPSPEPIYNSEGKRLNTREFRTRKKLEEERHTLITEMVALNPDFKPPADYKPPATRVSDKV
  MIPQDEYPEINFVGLLIGPRGNTLKNIEKECNAKIMIRGKGSVKEGKVGRKDGQMLPGEDEPLHALVTAN
  TMENVKKAVEQIRNILKQGIETPEDQNDLRKMQLRELARLNGTLREDDNRILRPWQSSETRSITNTTVCT
  KCGGAGHIASDCKFQRPGDPQSAQDKARMDKEYLSLMAELGEAPVPASVGSTSGPATTPLASAPRPAAPA
  SNPPPPSLMSTTQSRPPWMNSGPSENRPYHGMHGGGPGGPGGGPHSFPHPLPSLTGGHGGHPMQHNPNGP
  PPPWMQPPPPPMNQGPHPPGHHGPPPMDQYLGSTPVGSGVYRLHQGKGMMPPPPMGMMPPPPPPPSGQPP
  PPPSGPLPPWQQQQQQPPPPPPPSSSMASSTPLPWQQRSLPAAAMARAMRVRTFRAHW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_058210
• ORF Size: 1644 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_058210.2, NP_478117.2
• RefSeq Size: 2762 bp
• RefSeq ORF: 1647 bp
• Locus ID: 117855
• Cytogenetics: 1q43
• MW: 59.7 kDa
• Gene Summary: mRNA expression increases significantly following transient forebrain ischemia; may be activated following p53 induced apoptosis [RGD, Feb 2006]