Rpp14 (NM_001141929) Rat Tagged ORF Clone

CAT#: RR200075

Rpp14 (Myc-DDK-tagged ORF) - Rat ribonuclease P 14 subunit (human) (Rpp14), transcript variant 2, (10 ug)

Product Images

Circular map for RR200075

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rpp14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >NM_001141929 ORF sequence, RR200075 may differ due to SNPs.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCAGCTACTGCTTATGAAAGGGTAGTTTATAAGAGCCCTTCGGAGTACCACTACATGAAAGTCTGC
  TTAGAGTTTCAGGAACATGGGGTTGGACTGAATGGAGCCCAGTTCAAACAGCTACTTGTTTCAGCGCTG
  AAAGACCTGTTTGGGGAGGTTGGCGCTGCTTTACCTGTGGATGTTCTCACTTATGATGAGACCACACTG
  TCAGCCATCTTGAGAGTATGCAGCAGTGGTCTGGCCAAATTGTGGAGCTCTTTGACCCTGTTTGGAGCC
  TATAAAGGCAAGAAATGTGCCTTCAGAGTGAGTCAGGTGTCTCCCTTTCTCCTCGCGTTATCTGGCAAT
  AGCAGGGAACAAGTATTGGAC
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >Peptide sequence encoded by RR200075
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MPATAYERVVYKSPSEYHYMKVCLEFQEHGVGLNGAQFKQLLVSALKDLFGEVGAALPVDVLTYDETTL
  SAILRVCSSGLAKLWSSLTLFGAYKGKKCAFRVSQVSPFLLALSGNSREQVLD
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001141929
• ORF Size: 366 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001141929.2
• RefSeq Size: 1823 bp
• RefSeq ORF: 369 bp
• Locus ID: 361020
• Cytogenetics: 15p14
• MW: 13.4 kDa