Dnmt3a (NM_001003957) Rat Tagged ORF Clone

CAT#: RR200036

Dnmt3a (Myc-DDK-tagged ORF) - Rat DNA (cytosine-5-)-methyltransferase 3 alpha (Dnmt3a), transcript variant 2, (10 ug)

Product Images

Circular map for RR200036

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dnmt3a
• Synonyms: MGC109195
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200036 representing NM_001003957
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGCTGTGGAGGAAAGCCAGGCCTCTGGGGAGTCCCAGAAGGTGGAGGAGGCCAGCCCTCCTGCAG
  TGCAGCAGCCCACGGACCCTGCATCTCCCACTGTGGCCACCACCCCTGAGCCAGTAGGGGCTGATGCTGG
  GGACAAGAATGCTACCAAGGCAGCTGATGACGAGCCCGAGTATGAGGATGGCCGGGGCTTTGGCATTGGA
  GAGCTGGTGTGGGGGAAACTCCGGGGCTTCTCCTGGTGGCCAGGCCGAATTGTGTCTTGGTGGATGACAG
  GCCGGAGCCGAGCAGCCGAAGGCACTCGCTGGGTCATGTGGTTCGGAGATGGCAAATTCTCAGTGGTGTG
  TGTCGAGAAGCTCATGCCCCTGAGCTCCTTCTGCAGTGCGTTCCACCAGGCCACCTACAACAAGCAGCCC
  ATGTACCGCAAAGCCATCTACGAAGTCCTCCAGGTGGCCAGCAGCCGTGCAGGGAAGCTGTTCCCAGCAT
  GTCATGACAGCGATGAAAGTGACACTGGCAAGGCTGTGGAGGTGCAGAACAAGCAGATGATTGAGTGGGC
  CCTTGGCGGCTTCCAGCCCTCTGGTCCCAAAGGCCTGGAGCCACCAGAAGAGGAGAAGAATCCTTACAAG
  GAAGTTTACACCGACATGTGGGTGGAGCCTGAGGCAGCTGCTTATGCCCCACCCCCACCAGCCAAGAAAC
  CCAGAAAGAGCACAACAGAGAAACCCAAGGTCAAGGAGATCATTGATGAACGCACAAGAGAACGGCTCGT
  GTATGAGGTGCGCCAGAAGTGCCGAAACATCGAGGACATTTGTATCTCATGTGGGAGCCTCAATGTTACC
  CTGGAGCACCCACTCTTCATTGGTGGAATGTGCCAGAACTGTAAGAACTGCTTCTTGGAGTGTGCTTACC
  AATACGATGACGATGGGTACCAGTCCTACTGTACCATCTGCTGTGGGGGGCGCGAAGTGCTCATGTGTGG
  GAACAACAACTGCTGCAGGTGCTTTTGTGTGGAGTGTGTGGATCTCTTGGTGGGGCCAGGGGCTGCCCAA
  GCAGCCATTAAGGAAGACCCCTGGAACTGCTACATGTGTGGGCACAAGGGCACGTATGGGCTGCTGCGGA
  GACGGGAGGACTGGCCTTCTCGACTCCAGATGTTCTTCGCCAATAACCACGACCAGGAATTTGACCCCCC
  GAAGGTTTACCCACCTGTGCCAGCTGAGAAGAGGAAGCCCATCCGGGTGCTATCTCTCTTTGATGGGATT
  GCTACAGGGCTCCTGGTGCTGAAGGACCTGGGCATCCAAGTGGACCGCTACATCGCCTCCGAGGTGTGCG
  AGGACTCCATCACAGTGGGCATGGTGCGGCACCAGGGAAAGATCATGTACGTCGGGGACGTCCGCAGCGT
  CACACAGAAGCATATCCAGGAGTGGGGCCCATTCGATCTGGTGATTGGGGGCAGTCCCTGCAATGACCTC
  TCCATCGTCAACCCTGCCCGCAAGGGACTTTACGAGGGCACGGGCCGCCTCTTCTTTGAGTTCTACCGCC
  TCCTGCATGACGCGCGGCCCAAGGAGGGAGATGATCGCCCCTTCTTCTGGCTTTTTGAGAATGTGGTGGC
  CATGGGCGTTAGTGACAAGAGGGACATCTCGAGATTTCTTGAGTCTAACCCCGTGATGATCGACGCCAAA
  GAAGTGTCTGCTGCACACAGGGCCCGTTATTTCTGGGGTAACCTTCCTGGCATGAACAGGCCATTGGCAT
  CCACTGTGAATGATAAGCTGGAGTTGCAAGAGTGTCTGGAACACGGCAGAATAGCCAAGTTCAGCAAAGT
  GAGGACCATTACCACCAGGTCAAACTCCATAAAGCAGGGCAAAGACCAGCATTTCCCTGTCTTCATGAAT
  GAGAAGGAAGACATCCTCTGGTGCACTGAAATGGAAAGGGTGTTTGGCTTCCCTGTCCACTACACAGACG
  TCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGCCGATCGTGGAGCGTGCCAGTCATCCGCCA
  CCTCTTCGCTCCGCTGAAGGAATATTTTGCTTGTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200036 representing NM_001003957
  Red=Cloning site Green=Tags(s)
  
  MNAVEESQASGESQKVEEASPPAVQQPTDPASPTVATTPEPVGADAGDKNATKAADDEPEYEDGRGFGIG
  ELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVCVEKLMPLSSFCSAFHQATYNKQP
  MYRKAIYEVLQVASSRAGKLFPACHDSDESDTGKAVEVQNKQMIEWALGGFQPSGPKGLEPPEEEKNPYK
  EVYTDMWVEPEAAAYAPPPPAKKPRKSTTEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVT
  LEHPLFIGGMCQNCKNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGAAQ
  AAIKEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGI
  ATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKHIQEWGPFDLVIGGSPCNDL
  SIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFENVVAMGVSDKRDISRFLESNPVMIDAK
  EVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMN
  EKEDILWCTEMERVFGFPVHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001003957
• ORF Size: 2067 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003957.1, NP_001003957.1
• RefSeq Size: 3491 bp
• RefSeq ORF: 2070 bp
• Locus ID: 444984
• UniProt ID: Q1LZ53
• Cytogenetics: 6q14
• MW: 77.8 kDa
• Gene Summary: member of a family of DNA methyltransferases [RGD, Feb 2006]