Dhx15 (NM_001191597) Rat Untagged Clone

CAT#: RN217212

Dhx15 (untagged) - Rat DEAH (Asp-Glu-Ala-His) box helicase 15 (Dhx15)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Dhx15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217212 representing NM_001191597
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAAGAGGCATCGGTTGGACCTGGGGGAGGATTACCCCTCCGGCAAGAAGCGTGCGGGGACCGATG
  GGAAAGACCGGGAACGAGATCGGGATAGAGAGGACCGATCTAAAGATCGAGACCGAGAACGTGATAGAGG
  AGACAGAGAACGGGAGAGGGAGAAAGAAAAGGAAAAGGAATTGAGAGCTTCCACCAATGCTATGCTTATC
  AGTGCTGGATTGCCACCTTTAAAAGCTTCTCATTCCGCTCATTCAACCCACTCCGCTCATTCAACACATT
  CTACACATTCTGCCCATTCAACACACACCGGACATGCAGGACACACATCACTTCCACAGTGTATTAATCC
  ATTTACCAACCTGCCCCATACTCCTCGATATTATGATATTCTAAAGAAACGTCTTCAGCTCCCTGTTTGG
  GAATACAAGGATAGATTTACAGATATTCTTGTTAGACATCAGTCATTTGTACTGGTTGGTGAGACTGGGT
  CTGGTAAAACAACACAGATACCACAGTGGTGTGTGGAGTATATGCGATCCTTGCCAGGACCCAAAAGGGG
  AGTTGCCTGTACACAGCCCAGGAGAGTGGCTGCAATGAGTGTGGCTCAGAGAGTTGCTGATGAGATGGAC
  GTGATGTTAGGCCAGGAAGTTGGATACTCCATTCGATTTGAAGACTGCAGTAGTGCAAAAACCATTCTTA
  AGTATATGACTGACGGGATGCTCCTTCGTGAAGCTATGAATGATCCCCTCCTGGAGCGTTATGGTGTGAT
  AATTCTTGATGAGGCCCATGAAAGAACATTAGCTACGGATATTCTTATGGGTGTTCTAAAGGAAGTTGTG
  AGACAGAGATCAGATTTAAAGGTTATAGTTATGAGTGCTACCCTAGATGCTGGAAAATTCCAGATTTACT
  TTGATAACTGTCCTCTCTTAACTATACCTGGCCGAACACATCCTGTTGAGATTTTTTATACTCCCGAACC
  AGAGAGAGATTATCTTGAAGCAGCAATTCGAACGGTGATCCAAATACATATGTGTGAAGAGGAGGAAGGA
  GACCTTCTTCTTTTCTTGACTGGTCAAGAGGAAATTGATGAGGCCTGTAAGAGAATAAAGCGTGAAGTTG
  ATGATTTGGGTCCTGAAGTTGGTGATATTAAAATCATTCCATTATATTCTACACTTCCACCCCAGCAGCA
  ACAGCGCATTTTTGAGCCACCACCTCCAAAAAAACAGAATGGAGCAATTGGAAGAAAGGTGGTTGTGTCA
  ACTAATATTGCAGAGACCTCTTTGACAATAGATGGTGTGGTGTTTGTTATTGACCCTGGATTTGCAAAGC
  AAAAGGTATATAACCCTAGAATCAGAGTTGAGTCTCTTTTGGTGACAGCAATTAGTAAAGCCTCAGCTCA
  GCAAAGGGCTGGTCGAGCTGGACGTACCAGACCTGGGAAATGCTTCAGGCTGTACACAGAGAAAGCTTAT
  AAAACAGAAATGCAGGATAACACCTATCCTGAGATTCTGCGTTCTAATTTAGGATCAGTTGTATTGCAAT
  TGAAGAAGCTTGGTATTGATGACTTGGTACATTTTGATTTTATGGACCCACCAGCTCCCGAAACTCTGAT
  GAGAGCGCTAGAACTTCTGAATTATTTGGCCGCTTTAAATGATGATGGAGATCTGACTGAATTGGGATCC
  ATGATGGCAGAATTTCCTTTAGATCCCCAGCTCGCTAAAATGGTTATTGCAAGTTGTGACTACAACTGTT
  CTAATGAGGTCCTATCTATTACTGCCATGTTGTCAGTCCCACAGTGTTTTGTTCGCCCCACGGAGGCCAA
  GAAAGCTGCAGATGAGGCCAAGATGAGATTTGCCCACATAGATGGAGATCATCTGACACTGCTGAATGTT
  TACCACGCTTTTAAACAAAATCATGAATCTGTTCAGTGGTGTTATGACAACTTCATTAACTACCGGTCCC
  TGATGTCTGCAGACAATGTACGACAGCAGCTATCAAGAATTATGGACAGATTTAATTTGCCTCGTCGAAG
  TACTGATTTCACAAGCAGGGACTATTATATTAATATAAGAAAAGCATTGGTTACTGGGTATTTTATGCAG
  GTGGCACATTTAGAACGAACAGGGCATTACTTAACTGTGAAAGATAACCAGGTGGTCCAGTTGCATCCCT
  CTACTGTGCTTGATCACAAGCCTGAATGGGTGCTTTATAATGAGTTTGTGCTCACAACAAAGAATTATAT
  CCGAACCTGTACAGATATCAAGCCAGAATGGTTGGTGAAAATTGCCCCTCAATATTATGACATGAGCAAT
  TTCCCACAGTGTGAAGCAAAGAGACAGTTGGACCGCATTATTGCCAAACTTCAGTCCAAGGAATATTCAC
  AGTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001191597
• Insert Size: 2388 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001191597.1, NP_001178526.1
• RefSeq Size: 3007 bp
• RefSeq ORF: 2388 bp
• Locus ID: 289693
• Cytogenetics: 14q11