Mier3 (NM_001168000) Rat Untagged Clone

CAT#: RN216832

Mier3 (untagged) - Rat mesoderm induction early response 1, family member 3 (Mier3)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Mier3
• Synonyms: RGD1311241
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216832 representing NM_001168000
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGTCCATGACTATGATGATGAAAGAACTCTAGAGGAAGAGGAATTGATGGGTGACGGCAAAAACT
  TCAGTTCTGAAATTGAAGACCTGGAAAAGGAAGGAAATATGCCTCTAGAAGATCTGTTGGCATTCTACGG
  CTATGAATCTACAATTCCAACGGTTGCAAATTCCAGTGCAAACAGCTCCCCAAGTGAACTTGCAGACGAA
  CTACCAGATATGACTCTAGACAAAGAAGAAATAGCAAAAGACCTGTTGTCGGGCGATGATGAGGAAACCC
  AGTCTTCTGCAGATGACCTGACACCGTCTGTGACTTCCCATGAAACTTCAGACTTCTTCCCGCGGCCTTT
  ACGATCAAACACAACATGTGATGGAGATAAGGAATCAGAGATTGAGGACGTTGAAACGGACAGTGGTAAC
  TCACCTGAAGATTTGAGAAAGGAAATAATGATTGGTCTACAGTATCAGGCAGAAATTCCCCCTTATCTTG
  GCGAGTACAGTGGTGATGAGAAAGCTTATGAAAATGAAGACCAGTTACTGTGGCGTCCTGATGTGGTTTT
  GGAGAGCAAAGTCAAGGAGTACCTTATTGAGACCTCATTAAGAACTGGAAATGAAAAAGTCCTAGACAGA
  ATTTCCTCAGGCACACACACCAGGGATAACGAACAGGCATTATATGAACTTCTCAAGTGTAACCACAATG
  TTACGGAGGCAATCGAAAGGTACTGCTGTAATGGAAAGGCGTCTCAAGAAGGAATGACAGCTTGGACTGA
  AGAGGAGTGCCGGAGTTTTGAACATGCGCTAATGCTTTATGGGAAAGATTTTCATCTCATTCAGAAGAAT
  AAGGTGAGATCGAGAACTGTTGCTGAATGTGTAGCCTTCTACTACATGTGGAAGAAGTCAGAACGCTACG
  ATTACTTTGCTCAACAGACAAAATTTGGAAAGAAACGCTATAACCATCATCCTGGCGTTACGGACTATAT
  GGATCGTCTTGTAGATGAAACAGAGTCTCTGGGTGATACGGTCAGTTCTTCAGCCTTAACTTCTAACCGG
  CCTGAGCCCATTCCTGATCAGCCGCTGAACATTCTCAGCTCCTTCACTGCCAGTGACTTGACAGCTTTGA
  CCAACAGTGTAGCAACTGTCTGCAACCCCACAGATGTGAGTTGTCTGGATGATAGCTTTTCTCCACTGGG
  GAACGCGCCCCGTGGACAAGTCAATCATGTGCCTGTCGTAACGGAAGAGTTACTCACCCTGCCCAGCAAT
  GGTGAAAGTGATTGTTTTAACTTATTTGAGACTGGATTTTATCACTCAGAGTTAAACCCCATGTGCAGCG
  AAGAATCGGAAAGACCAGCAAAGAGATTGAAAATGGGCATTGCTGTCCCGGAATCCTTTATGAATGAGGT
  TTCTGTTAATAACCTGGGTGTAGACTTTGAAAATCACACACATCACATCACCAGTGCCAAAATGGCTGTT
  TCTGTGGCTGACTTTGGCAGCTTGTCTGCCAGTGAGACAAACGGATTCATCAATGCCCACACTCTACATC
  AGCACGCAGCCCTTCATTCAGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001168000
• Insert Size: 1566 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001168000.1, NP_001161472.1
• RefSeq Size: 4758 bp
• RefSeq ORF: 1566 bp
• Locus ID: 310086
• Cytogenetics: 2q14