Pparg (NM_001145367) Rat Untagged Clone

CAT#: RN216752

Pparg (untagged) - Rat peroxisome proliferator-activated receptor gamma (Pparg), transcript variant 3

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Pparg
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216752 representing NM_001145367
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTGACACAGAGATGCCATTCTGGCCCACCAACTTCGGAATCAGCTCTGTGGACCTCTCTGTGATGG
  ATGACCACTCCCATTCCTTTGACATCAAACCCTTTACCACGGTTGATTTCTCCAGCATTTCTGCTCCACA
  CTATGAAGACATCCCGTTCACAAGAGCTGACCCAATGGTTGCTGATTACAAATATGACCTGAAGCTCCAA
  GAATACCAAAGTGCGATCAAAGTAGAGCCTGCGTCCCCGCCTTATTATTCTGAAAAAACCCAACTCTACA
  ACAGGCCACATGAAGAGCCTTCAAACTCCCTCATGGCCATCGAGTGCCGAGTCTGTGGGGATAAAGCATC
  AGGCTTCCACTATGGAGTCCATGCTTGTGAAGGATGCAAGGGTTTTTTCCGAAGAACCATCCGATTGAAG
  CTTATTTATGATAGGTGTGATCTTAACTGTCGGATCCACAAAAAGAGTAGAAATAAATGTCAGTACTGTC
  GGTTTCAGAAGTGCCTTGCTGTGGGGATGTCTCACAATGCCATCAGGTTTGGGCGAATGCCACAGGCCGA
  GAAGGAGAAGCTGTTGGCGGAGATCTCCAGTGATATCGACCAGCTGAACCCAGAGTCTGCTGATCTGCGA
  GCCCTGGCAAAGCATTTGTATGACTCATACATAAAGTCCTTCCCGCTGACCAAAGCCAAGGCGAGGGCGA
  TCTTGACAGGAAAGACAACAGACAAATCACCATTTGTCATCTACGACATGAATTCCTTAATGATGGGAGA
  AGACAAAATCAAGTTCAAACATATCACCCCCCTGCAGGAGCAGAGCAAAGAGGTGGCCATCCGCATTTTT
  CAAGGGTGCCAGTTTCGATCCGTGGAAGCTGTGCAAGAGATCACAGAGTATGCCAAAAATATCCCTGGTT
  TCATTAACCTTGACTTGAATGACCAAGTGACTCTGCTCAAGTATGGTGTCCATGAGATCATCTACACCAT
  GCTGGCCTCCCTGATGAATAAAGATGGAGTCCTCATATCAGAGGGACAAGGATTCATGACCAGGGAGTTC
  CTCAAAAGCCTGCGGAAGCCCTTTGGTGACTTTATGGAGCCTAAGTTTGAGTTTGCTGTGAAGTTCAATG
  CACTGGAATTAGATGACAGTGACTTGGCCATATTTATAGCTGTCATTATTCTCAGTGGAGACCGCCCAGG
  CTTGCTGAACGTGAAGCCCATCGAGGACATCCAAGACAACCTGCTGCAGGCCCTGGAACTCCAGCTGAAG
  CTGAACCACCCGGAGTCCTCCCAGCTGTTCGCCAAGGTGCTCCAGAAGATGACAGACCTCAGGCAGATTG
  TCACAGAGCACGTGCAGCTACTGCATGTGATCAAGAAGACGGAGACAGATATGAGCCTTCACCCTCTGCT
  CCAGGAGATCTACAAGGACTTGTATTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001145367
• Insert Size: 1428 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145367.1, NP_001138839.1
• RefSeq Size: 2008 bp
• RefSeq ORF: 1428 bp
• Locus ID: 25664
• UniProt ID: O88275
• Cytogenetics: 4q42
• Gene Summary: ligand-activated transcription factor; mediates expression of genes involved in lipid metabolism [RGD, Feb 2006]
  Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. Both variants 2 and 3 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.