Slc1a3 (NM_001289943) Rat Untagged Clone

CAT#: RN216654

Slc1a3 (untagged) - Rat solute carrier family 1 (glial high affinity glutamate transporter), member 3 (Slc1a3), transcript variant Glast1c

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Slc1a3
• Synonyms: EAAT1; GLAST; GLAST-1; GluT-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216654 representing NM_001289943
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAAAAAGCAACGGAGAAGAGCCCAGGATGGGAAGCAGGATGGAAAGATTCCAGCAAGGGGTGCGCA
  AGCGGACGCTCCTGGCCAAGAAGAAAGTTCAGAACATCACCAAGGAGGATGTGAAGAGCTACCTGTTTCG
  GAATGCCTTTGTGCTACTCACCGTCAGCGCTGTCATTGTGGGTACAATCCTTGGATTTGCCCTCCGACCG
  TATAAAATGAGCTACCGGGAGGTCAAGTACTTCTCCTTTCCTGGGGAGCTTCTGATGCGGATGCTGCAGA
  TGTTGGTCTTACCCCTGATCATCTCCAGTCTTGTCACAGGAATGGCGGCCCTAGATAGTAAGGCATCTGG
  GAAGATGGGGATGCGAGCTGTGGTCTATTACATGACTACCACCATCATTGCTGTGGTGATCGGCATAATC
  ATTGTCATCATCATCCACCCCGGAAAGGGCACGAAAGAAAACATGTACAGAGAAGGTAAAATCGTGCAGG
  TGACTGCCGCAGATGCCTTCCTGGATTTAATCAGGTATGCACCTCTGGGCATCCTCTTCTTGATCGCAGG
  GAAGATTGTTGAGATGGAAGATATGGGTGTGATTGGGGGGCAGCTTGCCATGTATACAGTGACAGTCATC
  GTCGGCCTCCTCATTCATGCCGTCATCGTCCTGCCTCTCCTCTACTTCCTGGTAACCCGGAAGAACCCCT
  GGGTTTTCATTGGAGGGTTGCTGCAAGCACTCATCACAGCCCTGGGGACCTCCTCAAGTTCTGCCACCCT
  GCCCATCACTTTCAAGTGCCTGGAAGAAAACAATGGTGTGGACAAACGCATCACCAGATTTGTGCTTCCC
  GTGGGGGCCACCATTAACATGGATGGGACCGCCCTCTACGAGGCTTTGGCCGCCATTTTCATCGCTCAAG
  TTAACAACTTTGACCTGAATTTTGGACAGATTATAACAATAAGTATCACAGCCACAGCCGCAAGCATTGG
  GGCAGCTGGCATTCCTCAGGCCGGTCTAGTCACCATGGTCATCGTGCTGACATCTGTGGGCCTGCCCACG
  GATGACATCACACTCATCATTGCAGTGGACTGGTTTCTGGACCGCCTCCGAACCACCACCAACGTGCTGG
  GTGACTCCCTCGGGGCCGGGATTGTTGAACACTTGTCCCGACATGAACTGAAGAACCGAGATGTTGAAAT
  GGGGAACTCCGTGATTGAGGAGAATGAAATGAAAAAGCCGTATCAGCTGATTGCCCAGGACAATGAACCA
  GAGAAACCCGTGGCAGACAGCGAAACCAAGATGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289943
• Insert Size: 1296 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289943.1, NP_001276872.1
• RefSeq Size: 3596 bp
• RefSeq ORF: 1296 bp
• Locus ID: 29483
• UniProt ID: P24942
• Cytogenetics: 2q16
• Gene Summary: carries out Na+ dependent transport of glutamate and aspartate; may regulate neurotransmitter concentration in the central nervous system [RGD, Feb 2006]
  Transcript Variant: This variant (Glast1c, PMID:22026960) lacks two alternate in-frame exons in the coding region, compared to variant Glast. The encoded protein (isoform 4) is shorter, compared to isoform 1.