Phlda1 (NM_017180) Rat Untagged Clone

CAT#: RN214636

Phlda1 (untagged ORF) - Rat pleckstrin homology-like domain, family A, member 1 (Phlda1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Phlda1
• Synonyms: Tdag
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN214636 representing NM_017180
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCGTACGCCGGCTGCCGAGCGCCTCTCGGAGCTGGGTTTTCCCCCGCGGCGCGGGAGCCAGGAGC
  CGCCTTTTCCGCTGGGTGTCACTCGGGGGTGGGGAGGGTGGCCCATTGAAAAGCGCTGCGAGGGGCCCCG
  GCCAGTGCCCTTCAGTGAGCGCTCCGCGGAAGACGGCAGAGAGCAGCCCGCTCACGGCTCCGGGATCTTG
  TGGCGGGTCAGGACTCGCCTGTCCCTCTGCCGGGACCCCGAGCCACCGCCGCCACCGCCACCGCTCTGTC
  TCCTGCGAGTCAGCCTTCTCTGTGCGCTCCGGGCAGGCGGCCGCGGAAGCCGCTGGAGCGAGGACAGCGC
  ACGGCTGCTACTGCTACCCCCAGCCGGAGCGTCTGGAAGCCTAAAGGCCGAGCGGAGCAGCAGCACCCCT
  TATGCCGGAAGGATGCTGGAGAGCAGCGGTTGCAAGGCGCTGAAGGAAGGAGTGCTGGAAAAGCGAAGCG
  ACGGGCTGCTGCAGCTCTGGAAGAAAAAGTGCTGCATCCTCACTGAGGAAGGGCTACTGCTCATACCGCC
  CAAGCAGGTACAACATCAGCAGCAGCAACAGCAGCAGCAACAGCCCGGGCAGGGGACAGCCGAACCGTCC
  CAACCTAGTGGCCCCGCCGTCACCAGCCTTGAGCCACCAGCCAAGCTGAAGGAATTGCACTTTTCCAATA
  TGAAGACTGTAGACTGCGTGGAGCGCAAGGGCAAGTATATGTACTTCACTGTGGTGATGGCGGAGGGCAA
  AGAGATCGACTTTCGGTGTCCCCAGGACCAGGGCTGGAACGCAGAGATCACGTTGCAGATGGTGCAGTAC
  AAAAATCGTCAGGCCATACTGGCGGTCAAGTCTACGAGGCAGAAGCAGCAGCACCTGGTCCAGCAGCAGC
  CTCCGCAGACGCAGCAGATCCAACCCCAGCCCCAGCCGCAGATCCAACCCCAGCCCCAGCCGCAGATCCA
  GCCTCAACCCCAACCCCAACCCCAACCTCAGCCCCAACCCCAGCAGCAGCCCCAGCCCCAACAGCTCCAC
  TCCTACCCGCACCCGCATCCTCATCTTTACCCTCACCCTCACCCTCACGCGCACTCGCACCCGCACCCGC
  ACCCTCACCCTCATCCACACCAACTCCAGCATGCGCACCAGCCTCTCCACTCGCAGCCACAGGGCCACCG
  GCTTCTCCGCAGCACCTCCAACTCTGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_017180
• Insert Size: 1221 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017180.2, NP_058876.2
• RefSeq Size: 1811 bp
• RefSeq ORF: 1221 bp
• Locus ID: 29380
• Cytogenetics: 7q22
• Gene Summary: may mediate induction of apoptosis during neuronal development; induced by growth factors and differentiating agents [RGD, Feb 2006]