Ddx49 (NM_001115023) Rat Untagged Clone

CAT#: RN214603

Ddx49 (untagged ORF) - Rat DEAD (Asp-Glu-Ala-Asp) box polypeptide 49 (Ddx49), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Ddx49
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN214603 representing NM_001115023
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGGTTCGCTGAGCTCCGGCTATCGTCGTGGCTGGTGGAACAGTGTCGACAGCTGGGGTTGAAGC
  AGCCCACGCCGGTGCAGCTCGGGTGCATCCCGGCCATCCTGGAAGGTCGGGACTGCTTGGGTTGTGCAAA
  AACAGGAAGTGGCAAGACAGCAGCCTTTGTACTTCCCATCCTGCAGAAGCTGTCTGAGGACCCTTATGGC
  ATCTTCTGCCTGGTTTTGACACCCACTAGAGAGCTGGCCTACCAGATAGCTGAGCAGTTCCGAGTGCTGG
  GGAAGCCTCTGGGCCTAAAGGACTGCATCATCGTTGGTGGCATGGACATGGTGACGCAGGCCCTGGAGCT
  CTCTCGGAAGCCACACGTGGTTATTGCCACCCCTGGGCGCCTGGCTGACCACCTGCGCAGTTCCAACACT
  TTCAACATGAAGAAAATCAGATTCTTGGTGTTGGACGAGGCCGATAGGCTGCTGGAGCAGGGTTGCACCG
  ACTTCACTGCAGACCTGGAGACCATCCTGTCAGCTGTTCCAGCACGCAGGCAAACCCTCCTGTTCAGTGC
  CACACTGACGGACACACTCAAGGAGCTGCAGGGCCTGGCTACCAACCAGCCCTTCTTTTGGGAGGCACAG
  GCCACGGTTCGCATGGTGGAGCAGCTTGACCAGCGCTATCTGCTGGTGCCTGAGAAGGTGAAGGACGCCT
  ACCTGGTTCACCTGGTCCAGACCTTCCAGGACCAGCTGGAGGACTGCTCCATCATCATCTTCACCAATAC
  CTGCAAGACCTGCCAGATCCTTTGTATGATGCTTCGAAAGTTCAACTTCCCCACAGTGGCACTGCACTCC
  ATGATGAAGCAGAAAGAGCGCTTTGCAGCCCTGGCCAGATTCAAGTCCAGCACCTATCGGATTCTTATTG
  CTACTGACGTGGCCTCCAGAGGCCTGGACATCCCCACTGTGCAGGTGGTCATCAACCACAACACCCCTGG
  GCTCCCCAAGATCTACATCCATAGGGTCGGTCGCACAGCCCGTGCAGGGAGGCAGGGACAGGCCATCACA
  CTGGTGACGCAGTACGACATCCACCTGTTGCATGCCATCGAGGAGGAGATCAAACAGCAGCTGGCTGAAT
  TGGCAGTGGAGGAGGCCCAGGTCCTGCAGATCCTCACACAGGTCAACGTGGTGCGCAGGGAGTGTGAAAT
  CAAACTGGAGGCTTCGCATTTTGATGAAAAGAAGGAGATCAACAAGCGGAAACAGATGATCCTGGAGGGG
  AAGGACCCGGACCTGGAGGCCAAGCGCAAAGCTGAGCTGGCCAAGATTAAGCAACAGAACCGGCGCTTCA
  AGGAGAAAGTGGAGCAGACGCTTCGGCAGCAGAAAGCAGGGAGCACAGGCCGGCGGTCGCGTCCTCCCAG
  ACCTCGTCCCCAAGAGCCAGCCAGGGCTGAGGCTCAGAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001115023
• Insert Size: 1443 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001115023.1, NP_001108495.1
• RefSeq Size: 1555 bp
• RefSeq ORF: 1443 bp
• Locus ID: 290660
• Cytogenetics: 16p14