Scn5a (NM_013125) Rat Untagged Clone

CAT#: RN214460

Scn5a (untagged ORF) - Rat sodium channel, voltage-gated, type 5, alpha subunit (Scn5a), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Scn5a
• Synonyms: Nav1.5; SCAL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN214460 representing NM_013125
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAACCTCCTGTTACCTCGGGGCACCAGCAGCTTCCGTAGGTTCACCCGGGAGTCACTGGCGGCCA
  TCGAGAAGCGAATGGCTGAAAAGCAAGCCCGAGGAGGTTCGGCCACCTCACAGGAGAGCCGTGAGGGCCT
  GCAGGAGGAGGAGGCTCCCCGGCCCCAGCTGGACCTACAGGCCTCCAAAAAGCTGCCAGATCTCTATGGC
  AACCCACCCCGAGAGCTCATCGGGGAGCCCCTGGAAGACCTGGACCCTTTCTATAGTACCCAGAAGACCT
  TCATCGTGCTGAATAAGGGCAAAACCATCTTCCGGTTCAGTGCCACCAATGCCTTGTATGTCCTCAGCCC
  CTTCCACCCCGTGCGCCGAGCGGCCGTGAAGATCCTGGTACACTCGCTCTTTAGCATGCTCATCATGTGC
  ACCATCCTGACCAACTGCGTGTTCATGGCCCAGCACGACCCTCCGCCTTGGACCAAATATGTTGAGTACA
  CCTTCACTGCCATCTACACCTTTGAGTCTCTGGTCAAGATTCTAGCTCGAGGCTTCTGCCTGCATGCATT
  CACCTTCCTTCGGGACCCGTGGAACTGGCTAGACTTCAGTGTGATCGTCATGGCATACACAACTGAATTT
  GTGGACCTGGGCAATGTCTCAGCCTTACGCACCTTCCGAGTCCTCCGGGCCCTGAAAACTATATCGGTCA
  TTTCAGGCCTGAAGACCATCGTGGGAGCCCTAATCCAGTCTGTGAAGAAACTGGCCGATGTGATGGTCCT
  CACTGTCTTCTGCCTCAGTGTCTTTGCCCTCATTGGCCTGCAGCTCTTCATGGGCAACCTGAGGCACAAG
  TGTGTGCGTAACTTCACCGAGCTCAATGGCACCAATGGTTCCGTGGAGGCCGACGGCCTAGTCTGGAACT
  CCCTGGACGTCTACCTCAATGACCCAGCCAATTACCTGCTCAAGAATGGCACCACGGATGTGTTACTATG
  TGGGAACAGCTCTGATGCCGGGACATGCCCTGAGGGCTATCGGTGCCTGAAGGCAGGTGAGAACCCAGAC
  CACGGTTACACCAGCTTCGACTCCTTCGCCTGGGCCTTCCTTGCACTCTTCCGCCTGATGACACAGGACT
  GCTGGGAACGCCTATACCAGCAGACCCTGAGGTCCGCAGGAAAGATCTACATGATCTTCTTCATGCTCGT
  CATCTTTCTGGGCTCCTTCTACCTGGTGAACTTGATCCTGGCTGTGGTGGCCATGGCCTACGAGGAGCAA
  AACCAAGCCACCATCGCCGAGACGGAAGAGAAGGAGAAGCGCTTCCAGGAGGCCATGGAGATGCTCAAGA
  AGGAACACGAGGCTCTCACCATCAGGGGTGTGGATACCGTGTCCCGTAGCTCTCTGGAGATGTCTCCTTT
  GGCCCCAGTAACCAACCATGAGAGAAAGAGCAAAAGGAGGAAACGACTATCTTCGGGGACAGAGGATGGT
  GGGGATGACAGGCTCCCCAAGTCGGACTCAGAAGATGGTCCCAGAGCATTGAATCAGCTCAGCCTCACCC
  ATGGGCTCAGCCGGACATCCATGAGGCCCCGCTCGAGCCGAGGGAGCATTTTCACGTTCCGAAGACGGGA
  CCAAGGCTCTGAGGCGGACTTCGCAGATGACGAGAACAGCACTGCGGGGGAGAGCGAGAGCCACCGCACA
  TCGCTGCTGGTACCCTGGCCCCTGCGCCATCCCAGCGCCCAAGGACAGCCCGGCCCTGGAGCCTCAGCTC
  CCGGTTACGTTCTCAATGGCAAAAGGAACAGCACCGTGGACTGCAATGGGGTGGTTTCCTTGCTGGGGGC
  AGGTGACGCAGAGGCCACCTCCCCAGGGAGCTACCTTCTCCGCCCTATGGTGCTGGACCGACCCCCAGAC
  ACGACCACTCCGTCAGAGGAGCCCGGTGGGCCCCAGATGCTGACACCTCAGGCTCCGTGTGCAGATGGTT
  TTGAGGAGCCCGGAGCACGGCAACGGGCACTCAGCGCTGTCAGTGTCCTCACCAGCGCCCTGGAAGAGTT
  GGAGGAGTCCCATCGGAAGTGTCCACCATGCTGGAACCGCTTTGCCCAGCACTACCTCATCTGGGAGTGC
  TGTCCACTCTGGATGTCCATCAAGCAGAAGGTGAAGTTTGTGGTCATGGACCCATTTGCCGACCTCACTA
  TCACCATGTGCATCGTGCTCAATACGCTCTTCATGGCTCTGGAGCATTACAACATGACGGCAGAGTTTGA
  GGAGATGCTGCAGGTCGGAAACCTGGTCTTCACGGGAATCTTCACAGCGGAGATGACCTTCAAGATCATC
  GCCCTTGACCCCTACTACTACTTCCAGCAGGGCTGGAATATCTTCGACAGCATCATCGTCATCCTCAGTC
  TCATGGAGCTGGGGCTGTCCCGCATGGGCAACTTGTCTGTGCTACGTTCCTTCCGTCTGCTGCGGGTCTT
  CAAGCTGGCCAAGTCCTGGCCCACCCTGAACACGCTCATCAAGATCATCGGAAACTCCGTGGGCGCCCTG
  GGGAACCTGACCCTGGTGCTGGCCATCATCGTCTTCATCTTCGCCGTGGTGGGCATGCAGCTCTTCGGCA
  AGAACTACTCAGAACTGAGGCACCGCATCAGCGACTCCGGCCTGCTGCCCCGCTGGCACATGATGGACTT
  TTTCCACGCCTTCCTCATCATCTTCCGCATCCTCTGTGGGGAGTGGATCGAGACCATGTGGGACTGCATG
  GAGGTGTCTGGGCAGTCGCTGTGCTTGCTGGTCTTCCTGCTCGTCATGGTCATTGGCAACCTTGTGGTCC
  TGAATCTCTTCTTGGCCTTGCTGCTCAGCTCCTTCAGCGCAGACAACCTCACAGCCCCTGACGAGGATGG
  GGAGATGAACAACCTCCAGCTGGCCCTGGCTCGCATCCAGAGGGGCCTGCGCTTTGTCAAGCGGACCACC
  TGGGACTTCTGCTGCGGGATCCTGCGGCGGCGACCTAAGAAGCCCGCGGCTCTTGCCACCCACAGCCAGC
  TGCCCAGCTGTATCACCGCCCCCAGGTCCCCACCACCCCCAGAGGTGGAGAAGGTGCCCCCAGCCCGCAA
  GGAAACACGATTCGAGGAGGACAAGCGACCCGGCCAGGGCACCCCTGGGGATTCGGAGCCTGTGTGTGTG
  CCCATCGCCGTGGCTGAGTCAGACACTGAAGACCAGGAAGAGGATGAAGAGAACAGCCTTGGCACAGAGG
  AAGAGTCCAGCAAACAGGAATCCCAAGTTGTGTCTGGTGGCCACGAGCCCTACCAGGAGCCCAGGGCCTG
  GAGCCAGGTGTCAGAGACCACGTCCTCTGAAGCTGGGGCCAGTACATCTCAGGCAGACTGGCAGCAAGAG
  CAGAAAACGGAGCCCCAGGCCCCGGGGTGCGGTGAGACCCCTGAGGACAGTTACTCCGAGGGCAGCACAG
  CTGACATGACCAACACCGCCGACCTCCTGGAGCAAATCCCAGACCTTGGTGAGGACGTCAAGGACCCAGA
  GGACTGCTTTACTGAAGGCTGCGTCCGACGCTGTCCCTGCTGCATGGTAGACACAACCCAGTCCCCAGGG
  AAGGTCTGGTGGCGATTGCGCAAGACCTGCTACCGCATCGTGGAGCACAGCTGGTTCGAGACTTTCATCA
  TCTTCATGATCCTGCTCAGCAGTGGAGCGCTGGCCTTCGAGGACATCTACCTGGAGGAGCGGAAGACCAT
  CAAGGTTCTGCTGGAGTACGCGGACAAGATGTTCACCTACGTCTTTGTGTTGGAGATGCTGCTCAAGTGG
  GTGGCCTACGGCTTCAAGAAGTACTTCACCAACGCCTGGTGCTGGCTGGACTTCCTGATTGTGGACGTCT
  CGCTGGTCAGCCTCGTGGCAAACACCTTAGGCTTCGCCGAAATGGGTCCCATCAAGTCACTGAGGACACT
  GCGTGCACTTCGACCCCTGAGGGCCTTGTCGAGATTTGAGGGCATGCGGGTGGTGGTCAATGCGCTGGTG
  GGCGCCATCCCCTCCATCATGAACGTCCTCCTCGTCTGCCTCATCTTCTGGCTCATCTTCAGCATCATGG
  GCGTGAACCTCTTCGCCGGGAAGTTCGGTAGGTGCATCAACCAGACAGAAGGGGACCTGCCTCTGAACTA
  CACCATCGTGAACAACAAGAGTGAGTGCGAGTCCTTCAACGTGACCGGAGAGTTGTACTGGACCAAGGTG
  AAGGTCAACTTTGACAACGTGGGAGCCGGGTACCTGGCCCTCCTGCAGGTGGCGACATTTAAAGGCTGGA
  TGGACATCATGTATGCGGCTGTGGACTCCAGAGGGTATGAGGAGCAGCCGCAGTGGGAAGACAACCTCTA
  CATGTACATCTACTTTGTCGTCTTCATCATCTTCGGCTCCTTCTTCACCCTCAACCTCTTCATCGGTGTC
  ATCATTGACAACTTCAACCAGCAGAAGAAAAAGTTAGGGGGCCAGGATATCTTCATGACGGAGGAGCAGA
  AGAAGTACTACAATGCCATGAAGAAGCTGGGCTCCAAGAAACCCCAGAAGCCCATCCCACGGCCCTTGAA
  CAAGTACCAGGGTTTCATATTCGACATTGTGACCAAGCAGGCCTTCGATGTCACCATCATGTTCCTCATC
  TGTTTGAACATGGTGACCATGATGGTGGAGACAGATGACCAGAGCCCTGAGAAGGTCAACATCTTGGCCA
  AGATCAACCTGCTCTTCGTGGCCATCTTCACAGGCGAGTGTATTGTCAAGATGGCTGCCCTGCGCCACTA
  TTACTTCACCAACAGCTGGAACATCTTCGACTTTGTGGTGGTCATCCTCTCCATTGTTGGCACTGTCCTC
  TCCGACATCATCCAGAAGTACTTCTTCTCCCCGACACTCTTCCGGGTCATCCGTCTGGCCAGGATCGGCC
  GCATCCTCAGGCTGATCCGCGGAGCCAAGGGGATTCGCACGCTGCTCTTCGCCCTCATGATGTCCCTGCC
  CGCCCTCTTCAACATCGGCCTCCTCCTCTTCCTCGTCATGTTCATCTACTCCATCTTCGGCATGGCCAAC
  TTCGCTTACGTCAAGTGGGAGGCCGGCATCGATGACATGTTCAACTTCCAGACCTTCGCCAACAGCATGC
  TGTGCCTGTTCCAGATCACCACATCAGCCGGCTGGGACGGCCTCCTCAGCCCCATCCTCAACACGGGGCC
  TCCCTACTGCGACCCCAACCTGCCCAACAGCAACGGCTCCCGGGGGAACTGTGGGAGCCCGGCGGTGGGC
  ATCCTCTTCTTCACCACCTACATCATCATCTCCTTCCTCATCGTGGTCAACATGTACATCGCCATCATCC
  TCGAGAACTTCAGCGTGGCCACCGAGGAGAGCACAGAGCCCCTGAGCGAGGACGACTTCGACATGTTCTA
  TGAGATCTGGGAGAAGTTCGACCCGGAGGCCACCCAGTTCATTGAGTATCTGGCCCTGTCCGACTTTGCA
  GATGCCTTGTCTGAGCCGCTCCGCATCGCCAAACCCAACCAGATAAGCCTCATCAACATGGATCTGCCCA
  TGGTGAGCGGAGACCGTATCCACTGTATGGACATACTGTTCGCTTTCACCAAGAGGGTGCTCGGCGAGTC
  TGGGGAGATGGATGCCCTGAAGATCCAGATGGAGGAGAAGTTCATGGCGGCCAACCCTTCCAAGATCTCC
  TACGAGCCCATCACCACCACCCTGAGGAGAAAGCACGAGGAGGTGTCGGCCACGGTCATCCAGCGTGCCT
  TCCGGAGGCACCTGCTGCAGCGCTCGGTGAAGCATGCCTCCTTTCTCTTCCGCCAGCAAGCGGGCGGCAG
  TGGCCTCTCCGACGAGGATGCCCCTGAGCGGGAGGGCCTCATCGCCTACATGATGAATGGGAACTTCTCT
  CGGCGCAGTGCTCCGCTCTCCAGCTCCTCCATCTCCTCCACGTCCTTCCCCCCGTCCTACGACAGCGTCA
  CGAGAGCCACCAGTGATAACCTCCCGGTGCGTGCGTCTGACTATAGCCGCAGCGAAGATCTTGCAGACTT
  CCCTCCATCTCCAGATAGGGACCGAGAGTCTATCGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013125
• Insert Size: 6060 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013125.2, NP_037257.1
• RefSeq Size: 8468 bp
• RefSeq ORF: 6060 bp
• Locus ID: 25665
• UniProt ID: P15389
• Cytogenetics: 8q32
• Gene Summary: acts as a tetrodotoxin-resistant voltage gated sodium channel; may play a role in cardiac function [RGD, Feb 2006]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).