Ppat (NM_057198) Rat Untagged Clone

CAT#: RN213445

Ppat (untagged ORF) - Rat phosphoribosyl pyrophosphate amidotransferase (Ppat), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Ppat
• Synonyms: Atase
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN213445 representing NM_057198
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACTGGAGGAGTCGGGGATCCGTGAGGAATGTGGTGTGTTCGGGTGCATCGCTTCTGGAGACTGGC
  CCACGCAGCTAGATGTGCCACATGTGATCACTCTGGGACTCGTGGGGCTTCAGCACCGAGGTCAGGAGAG
  CGCTGGTATTGTGACCAGTGATGGGAGTTCAGTGCCTAAATTCAGGGTGCATAAGGGAATGGGCCTTGTA
  AATCATGTTTTTACCGAAGACAATTTGAAGAAATTGTATGTCTCAAATCTTGGAATCGGACATACGCGGT
  ACGCCACCACAGGGAAGTGTGAGCTGGAAAATTGCCAGCCGTTTGTTGTTGAGACTCTGCATGGGAAAAT
  AGCTGTGGCCCATAACGGAGAGCTGGTGAACGCAGCTCGGCTGAGGAAGAAGCTTCTGCGGCATGGTATT
  GGGCTTTCCACATCCTCCGATAGTGAAATGATTACCCAGTTACTCGCATATACCCCTCCTCAGGAACAAG
  ATGATACTCCAGACTGGGTAGCAAGAATTAAGAACTTAATGAAGGAAGCGCCCGCAGCCTACTCCCTGGT
  CATAATGCACAGGGACGTCATTTACGCAGTGCGAGATCCATACGGAAATCGTCCTCTATGCATCGGCCGT
  CTTATGCCAGTTTCTGACATAAATGACAAAGAGAAAAAGTCTTCAGAGACTGAAGGATGGGTGGTGTCCT
  CAGAGTCTTGCAGCTTTTTATCTATTGGTGCGAGATATTGCCATGAAGTGAAGCCTGGGGAAATTGTAGA
  AATATCCAGACATGGTGTCAGAACTCTTGATATAATTCCAAGGTCTAATGGAGACCCAGTGGCTTTTTGT
  ATCTTCGAATATGTTTATTTTGCAAGACCAGATAGTATGTTTGAAGACCAGATGGTTTATACGGTAAGGT
  ACCGGTGTGGCCAGCAGCTGGCAGTTGAAGCACCAGTGGAGGCGGACCTGGTCAGCACAGTTCCAGAATC
  TGCCACGCCTGCGGCCCTGGGGTATGCGACAAAGTGTGGGCTGCCATATGTGGAGGTGCTGTGTAAGAAC
  CGGTATGTAGGAAGAACCTTCATTCAGCCAAACATGAGGCTAAGACAACTTGGGGTTGCAAAGAAATTCG
  GCGTGTTGTCTGACAACTTTAAAGGCAAAAGAATTGTTCTCATAGATGATTCCATTGTGAGAGGCAATAC
  TATCTCACCCATCATAAAACTCCTCAAAGAATCCGGTGCAAAGGAGGTACACATTCGAGTCGCTTCGCCA
  CCAATAAAACATCCGTGCTTCATGGGAATAAACATTCCTACAAAAGAAGAGCTTATAGCCAATAAGCCCG
  AATTTGAGTATCTTGCAGAGTACCTTGGAGCAAACAGCGTTGTGTATCTGTCAGTGGAAGGACTAGTTTC
  GTCTGTGCAACAAGAAATAAAGTTCAAGAAGCAGAAAGTTAAAAAGCGTGATATCACAATTCAAGAAAAT
  GGAAATGGTCTGGAGTATTTTGAGAAGACGGGACACTGTACGGCTTGCCTCACTGGCCAGTACCCTGTGG
  ATCTGGAATGGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_057198
• Insert Size: 1554 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_057198.2, NP_476546.1
• RefSeq Size: 4065 bp
• RefSeq ORF: 1554 bp
• Locus ID: 117544
• UniProt ID: P35433
• Cytogenetics: 14p11
• Gene Summary: catalyzes the irreversible reaction between 5- phosphoribosyl-1-pyrophosphate, glutamine, and water to form 5P-phosphoribosyl-1-amine, glutamate, and inorganic pyrophosphate [RGD, Feb 2006]