Prkce (NM_017171) Rat Untagged Clone

CAT#: RN212849

Prkce (untagged ORF) - Rat protein kinase C, epsilon (Prkce), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Prkce
• Synonyms: Pkce
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN212849 representing NM_017171
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAGTGTTCAATGGCCTTCTTAAGATCAAAATCTGCGAGGCCGTGAGCTTGAAGCCCACAGCCTGGT
  CGCTGCGCCATGCGGTGGGACCCCGGCCCCAGACGTTCCTTCTGGACCCCTACATTGCCCTTAACGTGGA
  CGACTCGCGCATCGGCCAAACAGCCACCAAGCAGAAGACCAACAGTCCGGCCTGGCACGATGAGTTCGTC
  ACTGATGTGTGCAATGGGCGCAAGATCGAGCTGGCTGTCTTTCACGATGCTCCTATCGGCTACGACGACT
  TCGTGGCCAACTGCACCATCCAGTTCGAGGAGCTGCTGCAGAATGGGAGCCGTCACTTCGAGGACTGGAT
  TGATCTGGAGCCAGAAGGAAAAGTCTACGTGATCATCGATCTCTCGGGATCATCGGGCGAAGCCCCTAAA
  GACAATGAAGAACGAGTGTTTAGGGAGCGGATGCGGCCAAGGAAGCGCCAAGGGGCTGTCAGGCGCAGGG
  TCCACCAGGTCAATGGCCACAAGTTCATGGCCACCTACTTGCGGCAGCCCACCTACTGCTCCCACTGTAG
  GGATTTCATCTGGGGTGTCATAGGAAAACAGGGATATCAATGTCAAGTTTGTACCTGCGTCGTCCACAAA
  CGATGCCATGAGCTCATTATTACGAAGTGCGCTGGGCTAAAGAAACAGGAAACCCCTGACGAGGTGGGCT
  CCCAACGCTTCAGCGTCAACATGCCCCACAAGTTCGGGATCCACAACTACAAGGTCCCCACGTTCTGTGA
  CCACTGTGGCTCCCTGCTCTGGGGCCTCTTGCGGCAGGGCCTGCAGTGTAAAGTCTGCAAAATGAATGTT
  CACCGTCGATGCGAGACCAACGTGGCTCCCAATTGTGGGGTGGACGCCAGAGGAATTGCCAAGGTGCTGG
  CCGATCTTGGCGTTACTCCAGACAAAATCACCAACAGTGGCCAGAGAAGGAAAAAGCTCGCTGCTGGTGC
  TGAGTCCCCACAGCCGGCTTCTGGAAACTCCCCATCAGAAGACGACCGATCCAAGTCAGCGCCCACCTCC
  CCTTGTGACCAGGAACTAAAAGAACTTGAAAACAACATCCGGAAGGCCTTGTCATTTGACAACCGAGGAG
  AGGAGCACCGAGCCTCGTCGTCTACTGATGGCCAGCTGGCAAGCCCTGGCGAGAACGGTGAAGTCCGGCA
  AGGCCAGGCCAAGCGCTTGGGCCTGGATGAGTTCAACTTCATCAAGGTGTTAGGCAAAGGCAGCTTTGGC
  AAGGTCATGCTGGCCGAGCTCAAGGGTAAGGATGAAGTCTATGCTGTGAAGGTCTTAAAGAAGGACGTCA
  TCCTGCAGGATGACGACGTGGACTGCACGATGACAGAGAAGAGGATTTTGGCTCTGGCGCGGAAACACCC
  TTATCTAACCCAACTCTATTGCTGCTTCCAGACCAAGGACCGGCTCTTCTTCGTCATGGAATATGTAAAC
  GGTGGAGACCTCATGTTCCAGATTCAGCGGTCCCGAAAATTCGATGAGCCTCGTTCCGGGTTCTATGCTG
  CCGAGGTCACATCTGCTCTCATGTTTCTCCACCAACATGGAGTGATCTACAGGGATTTGAAACTGGACAA
  CATCCTTCTAGATGCAGAAGGTCACTCCAAGCTGGCTGACTTTGGGATGTGCAAGGAAGGGATTCTGAAT
  GGCGTGACAACTACCACCTTCTGTGGGACTCCTGACTACATAGCTCCAGAGATCCTGCAGGAGTTGGAGT
  ACGGCCCCTCAGTGGACTGGTGGGCCCTGGGCGTGCTGATGTACGAGATGATGGCCGGGCAGCCCCCCTT
  TGAAGCTGACAACGAGGACGACTTGTTTGAATCCATCCTTCACGATGACGTTCTCTACCCTGTCTGGCTT
  AGCAAGGAGGCTGTCAGCATCCTGAAAGCTTTCATGACCAAGAACCCGCACAAGCGCCTGGGCTGCGTGG
  CAGCACAGAACGGGGAAGATGCCATCAAGCAACATCCATTCTTCAAGGAGATTGACTGGGTACTGCTGGA
  GCAGAAGAAAATGAAGCCCCCCTTCAAGCCGAGAATTAAAACCAAGAGAGATGTCAATAACTTTGACCAA
  GACTTTACCCGGGAAGAGCCAATACTTACACTTGTGGATGAAGCAATCGTGAAGCAGATCAACCAGGAAG
  AATTCAAAGGCTTCTCCTACTTTGGTGAAGACCTGATGCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_017171
• Insert Size: 2214 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017171.1, NP_058867.1
• RefSeq Size: 2704 bp
• RefSeq ORF: 2214 bp
• Locus ID: 29340
• UniProt ID: P09216
• Cytogenetics: 6q12
• Gene Summary: has phospholipid and diacylglycerol-dependent activity; plays a role in ouabain induced Na+/K+-ATPase mediated signal transduction in cardiac myocytes [RGD, Feb 2006]