Prkce (NM_017171) Rat Tagged ORF Clone

CAT#: RR212849

Prkce (Myc-DDK-tagged ORF) - Rat protein kinase C, epsilon (Prkce), (10 ug)

Product Images

Circular map for RR212849

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prkce
• Synonyms: Pkce
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212849 representing NM_017171
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAGTGTTCAATGGCCTTCTTAAGATCAAAATCTGCGAGGCCGTGAGCTTGAAGCCCACAGCCTGGT
  CGCTGCGCCATGCGGTGGGACCCCGGCCCCAGACGTTCCTTCTGGACCCCTACATTGCCCTTAACGTGGA
  CGACTCGCGCATCGGCCAAACAGCCACCAAGCAGAAGACCAACAGTCCGGCCTGGCACGATGAGTTCGTC
  ACTGATGTGTGCAATGGGCGCAAGATCGAGCTGGCTGTCTTTCACGATGCTCCTATCGGCTACGACGACT
  TCGTGGCCAACTGCACCATCCAGTTCGAGGAGCTGCTGCAGAATGGGAGCCGTCACTTCGAGGACTGGAT
  TGATCTGGAGCCAGAAGGAAAAGTCTACGTGATCATCGATCTCTCGGGATCATCGGGCGAAGCCCCTAAA
  GACAATGAAGAACGAGTGTTTAGGGAGCGGATGCGGCCAAGGAAGCGCCAAGGGGCTGTCAGGCGCAGGG
  TCCACCAGGTCAATGGCCACAAGTTCATGGCCACCTACTTGCGGCAGCCCACCTACTGCTCCCACTGTAG
  GGATTTCATCTGGGGTGTCATAGGAAAACAGGGATATCAATGTCAAGTTTGTACCTGCGTCGTCCACAAA
  CGATGCCATGAGCTCATTATTACGAAGTGCGCTGGGCTAAAGAAACAGGAAACCCCTGACGAGGTGGGCT
  CCCAACGCTTCAGCGTCAACATGCCCCACAAGTTCGGGATCCACAACTACAAGGTCCCCACGTTCTGTGA
  CCACTGTGGCTCCCTGCTCTGGGGCCTCTTGCGGCAGGGCCTGCAGTGTAAAGTCTGCAAAATGAATGTT
  CACCGTCGATGCGAGACCAACGTGGCTCCCAATTGTGGGGTGGACGCCAGAGGAATTGCCAAGGTGCTGG
  CCGATCTTGGCGTTACTCCAGACAAAATCACCAACAGTGGCCAGAGAAGGAAAAAGCTCGCTGCTGGTGC
  TGAGTCCCCACAGCCGGCTTCTGGAAACTCCCCATCAGAAGACGACCGATCCAAGTCAGCGCCCACCTCC
  CCTTGTGACCAGGAACTAAAAGAACTTGAAAACAACATCCGGAAGGCCTTGTCATTTGACAACCGAGGAG
  AGGAGCACCGAGCCTCGTCGTCTACTGATGGCCAGCTGGCAAGCCCTGGCGAGAACGGTGAAGTCCGGCA
  AGGCCAGGCCAAGCGCTTGGGCCTGGATGAGTTCAACTTCATCAAGGTGTTAGGCAAAGGCAGCTTTGGC
  AAGGTCATGCTGGCCGAGCTCAAGGGTAAGGATGAAGTCTATGCTGTGAAGGTCTTAAAGAAGGACGTCA
  TCCTGCAGGATGACGACGTGGACTGCACGATGACAGAGAAGAGGATTTTGGCTCTGGCGCGGAAACACCC
  TTATCTAACCCAACTCTATTGCTGCTTCCAGACCAAGGACCGGCTCTTCTTCGTCATGGAATATGTAAAC
  GGTGGAGACCTCATGTTCCAGATTCAGCGGTCCCGAAAATTCGATGAGCCTCGTTCCGGGTTCTATGCTG
  CCGAGGTCACATCTGCTCTCATGTTTCTCCACCAACATGGAGTGATCTACAGGGATTTGAAACTGGACAA
  CATCCTTCTAGATGCAGAAGGTCACTCCAAGCTGGCTGACTTTGGGATGTGCAAGGAAGGGATTCTGAAT
  GGCGTGACAACTACCACCTTCTGTGGGACTCCTGACTACATAGCTCCAGAGATCCTGCAGGAGTTGGAGT
  ACGGCCCCTCAGTGGACTGGTGGGCCCTGGGCGTGCTGATGTACGAGATGATGGCCGGGCAGCCCCCCTT
  TGAAGCTGACAACGAGGACGACTTGTTTGAATCCATCCTTCACGATGACGTTCTCTACCCTGTCTGGCTT
  AGCAAGGAGGCTGTCAGCATCCTGAAAGCTTTCATGACCAAGAACCCGCACAAGCGCCTGGGCTGCGTGG
  CAGCACAGAACGGGGAAGATGCCATCAAGCAACATCCATTCTTCAAGGAGATTGACTGGGTACTGCTGGA
  GCAGAAGAAAATGAAGCCCCCCTTCAAGCCGAGAATTAAAACCAAGAGAGATGTCAATAACTTTGACCAA
  GACTTTACCCGGGAAGAGCCAATACTTACACTTGTGGATGAAGCAATCGTGAAGCAGATCAACCAGGAAG
  AATTCAAAGGCTTCTCCTACTTTGGTGAAGACCTGATGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212849 representing NM_017171
  Red=Cloning site Green=Tags(s)
  
  MVVFNGLLKIKICEAVSLKPTAWSLRHAVGPRPQTFLLDPYIALNVDDSRIGQTATKQKTNSPAWHDEFV
  TDVCNGRKIELAVFHDAPIGYDDFVANCTIQFEELLQNGSRHFEDWIDLEPEGKVYVIIDLSGSSGEAPK
  DNEERVFRERMRPRKRQGAVRRRVHQVNGHKFMATYLRQPTYCSHCRDFIWGVIGKQGYQCQVCTCVVHK
  RCHELIITKCAGLKKQETPDEVGSQRFSVNMPHKFGIHNYKVPTFCDHCGSLLWGLLRQGLQCKVCKMNV
  HRRCETNVAPNCGVDARGIAKVLADLGVTPDKITNSGQRRKKLAAGAESPQPASGNSPSEDDRSKSAPTS
  PCDQELKELENNIRKALSFDNRGEEHRASSSTDGQLASPGENGEVRQGQAKRLGLDEFNFIKVLGKGSFG
  KVMLAELKGKDEVYAVKVLKKDVILQDDDVDCTMTEKRILALARKHPYLTQLYCCFQTKDRLFFVMEYVN
  GGDLMFQIQRSRKFDEPRSGFYAAEVTSALMFLHQHGVIYRDLKLDNILLDAEGHSKLADFGMCKEGILN
  GVTTTTFCGTPDYIAPEILQELEYGPSVDWWALGVLMYEMMAGQPPFEADNEDDLFESILHDDVLYPVWL
  SKEAVSILKAFMTKNPHKRLGCVAAQNGEDAIKQHPFFKEIDWVLLEQKKMKPPFKPRIKTKRDVNNFDQ
  DFTREEPILTLVDEAIVKQINQEEFKGFSYFGEDLMP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017171
• ORF Size: 2211 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017171.1, NP_058867.1
• RefSeq Size: 2704 bp
• RefSeq ORF: 2214 bp
• Locus ID: 29340
• UniProt ID: P09216
• Cytogenetics: 6q12
• MW: 83.5 kDa
• Gene Summary: has phospholipid and diacylglycerol-dependent activity; plays a role in ouabain induced Na+/K+-ATPase mediated signal transduction in cardiac myocytes [RGD, Feb 2006]