Ppp1cb (NM_013065) Rat Untagged Clone

CAT#: RN212543

Ppp1cb (untagged ORF) - Rat protein phosphatase 1, catalytic subunit, beta isoform (Ppp1cb), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Ppp1cb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN212543 representing NM_013065
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACGGGGAGCTGAACGTGGACAGCCTCATCACCCGCCTGCTGGAGGTACGAGGATGTCGTCCGG
  GAAAAATTGTGCAGATGACTGAAGCAGAAGTCCGAGGACTGTGTATCAAGTCTCGTGAAATCTTTCTTAG
  CCAGCCTATTCTTTTGGAATTGGAAGCGCCACTGAAGATTTGTGGAGATATTCATGGACAGTATACAGAC
  TTACTGAGATTATTTGAATATGGAGGTTTTCCACCAGAAGCCAACTATCTTTTCTTAGGAGATTATGTGG
  ACAGAGGAAAGCAGTCTTTGGAAACCATCTGTTTGCTATTGGCTTACAAAATCAAATACCCAGAGAACTT
  CTTTCTTCTACGAGGAAACCATGAGTGTGCTAGCATCAACCGCATTTATGGATTCTATGATGAGTGCAAA
  CGAAGATTTAATATTAAATTGTGGAAGACATTCACTGATTGTTTTAATTGTCTGCCTATAGCTGCTATTG
  TTGATGAGAAAATCTTCTGCTGTCATGGAGGACTGTCACCAGACCTACAGTCTATGGAACAGATTCGGAG
  AATTATGAGACCCACTGACGTACCTGATACAGGTTTGCTTTGTGATTTACTGTGGTCCGACCCAGATAAG
  GATGTACAAGGCTGGGGAGAAAATGATCGTGGTGTTTCTTTTACTTTTGGAGCTGATGTAGTCAGTAAAT
  TTCTGAATCGTCATGATTTGGACTTGATTTGTCGAGCCCATCAGGTGGTAGAAGATGGATATGAATTTTT
  TGCTAAACGACAATTGGTAACTTTATTTTCTGCCCCAAATTACTGCGGCGAGTTTGACAATGCTGGTGGT
  ATGATGAGTGTGGATGAAACTTTGATGTGTTCATTCCAGATATTGAAACCATCTGAAAAGAAAGCTAAGT
  ACCAGTATGGTGGGCTGAATTCTGGACGTCCTGTCACTCCGCCTCGAACAGCTAATCCACCGAAGAAAAG
  GTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013065
• Insert Size: 984 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013065.2, NP_037197.1
• RefSeq Size: 2942 bp
• RefSeq ORF: 984 bp
• Locus ID: 25594
• UniProt ID: P62142
• Cytogenetics: 6q13
• Gene Summary: one of several neuronal isoforms of protein phosphatase 1 [RGD, Feb 2006]