Rfc1 (NM_053547) Rat Untagged Clone

CAT#: RN212226

Rfc1 (untagged ORF) - Rat replication factor C (activator 1) 1 (Rfc1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Rfc1
• Synonyms: Recc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN212226 representing NM_053547
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACATTCGGAAATTCTTTGGGGTTATATCAAGTGGAAAAAAGCCTGTAAATGAGACAGTAAAGAAGA
  ATGAGAAGACAAAACCTTCGGAGGGCACTGTCAAAGGAAAGCAAGGAGTAAAGGAAGCCAAGGTTAATAA
  TCCCTGCAAAGAGGATGCCTCCAGACCAAAGCAGCACAACAAGAAAAAGAGGATCATCTATGACTCAGAT
  TCAGAATCAGAGGAGACAGTGCAAGTAAAAAATGCTAAAAAGAAATCAGAAAAATTGCCAGTGTCTTGTA
  AACCTGGTAAAATCTCTCGGAAGGATCCTGTTACCTATATTTCTGAGACAGATGAAGACGATGACTTTTT
  ATGTAAAAAGGCAGCCTCCAAATCAAAAGAGAATGGAGTATCTACAAACAGTTACCTTGGAGCATCAAAC
  GTGAAAAAGAATGAAGAAAACACTAAGACTAAGAGTAAACCGTTATCACCAATAAAACTAACACCGACAT
  CAGTGCTTGATTATTTTGGAACTGAAAGTGTCCAGAGATCTGGGAAGAAGATGGTGGCGAGCAAAAAGAA
  AGAGTCTTCTCAAACCCCAGATGATTCCAGATTAAATGATGAGGCCATCGCCAAGCAGCTTCAGCTTGAT
  GAAGATGCAGAGCTGGAGAGGCAGTTGCATGAAGATGAAGAATTTGCAAGAACACTGGCCTTGTTGGATG
  AAGAACCTAAGACCAAAAAGGCTCGAAAGGACTCTGAAGAGGGAGAATCATTTTCACCTGCCAAAGCTGA
  GTTAAGCAAAGCAGCAAAGCAGAAGAGCCCTGCTAACGAGCATTTCTCAATTGGAAGAAAGACCTACAGT
  CCTGCTAAGTACGGCAAGGGTAGAGGCTCAGAAGGCACCAAGCAGCCCTGCAGATCAGCTCACCAGAAGG
  AAGCCTGCTCCTCTCTCAAGGCCAGCTCCAAGTCGGCTCTTATGAAAGCACAAGAAGAAAATTCTTACAA
  AGAAACAGAACTGCTGGCTGCAAAAAGAGAAAGTGCCATTGAGCCCAAAGGAGAGAAAACAACTCCTAGG
  AAAACGAAAGGCTCTCCAACTAAAAGAGAGTCTGTAAGCCCAGAAGATTCTGAAAAGAAGCGCACCAATT
  ATCAAGCTTATCGAAGCTACTTAAATCGAGAAGGTCCCAAAGCCCTGGGCTCCAAAGAAATACCCAAGGG
  AGCTGAAAACTGCTTGGAGGGCCTGACGTTCGTGATCACCGGAGTGCTGGAGTCCATCGAACGAGAAGAA
  GCCAAGTCTCTAATTGAACGTTATGGGGGGAAAGTAACAGGAAACGTGAGCAAGAAAACCAGCTACCTCG
  TCATGGGCCGGGACAGCGGGCAGTCCAAGAGTGACAAGGCAGCAGCTCTGGGAACAAAAATCCTTGATGA
  AGACGGCCTGTTGGATCTGATTCGAACTATGCCGGGCAAAAAATCCAAGTACGAAATCGCTGCTGAGGCT
  GAGATGAAGAAAGAAAAGTCCAAATTGGAGAGAACACCCCAAAAAAATGATCAGGGAAAAAGAAAACTTA
  GTCCAACCAAGAGGGAGTTGGAGCCTAAAAAGAGCAAGCTGACTCCCCTTAAGCACAGCCCTCGGAAGGC
  AGTGAAGGAGGAGGAACGTGTGTGTCCGAGGGGCTTGGACGCCAAGGAGCCTCATGGCAGCCACAGTGCC
  AGCAGAGAGGAGTGCCTGCTCTGGGTGGATAAGTACAAGCCCACCTCACTCAAGAACATAATCGGACAGC
  AGGGTGACCAGAGCTGTGCCAACAAACTGCTACGCTGGCTCAGAAACTGGCACAAGAGTTCTCCAGAAGA
  GAAAAAGCATGCAGCAAAATTTGGCAAAGTTGCCAGCAAAGATGATGGCTCCAGTTTCAAGGCAGCACTG
  CTGTCTGGCCCTCCAGGTGTTGGCAAAACCACTACAGCTTCTCTTGTGTGTCAGGAATTGGGCTACAGTT
  ATGTGGAACTAAATGCGAGTGATACTCGGAGTAAGAACAGTCTGAAAGCAATTGTTGCTGAGTCATTGAA
  CAACACCAGCATCAAAGGCTTTTATACAAGTGGAGCATCTCCTTCTGTAAGTGCGAGGCATGCTCTCATC
  ATGGATGAAGTTGATGGCATGGCGGGCAATGAGGACAGGGGTGGAATTCAGGAGCTAATAGGCCTGATAA
  AGCATACGAAAATTCCCATCATTTGTATGTGCAATGATAGAAATCACCCCAAAATCCGCTCATTGGTTCA
  TTATTGTTTTGATCTTCGTTTCCAAAGACCTCGAGTTGAACAGATTAAGAGTGCAATGCTGTCTATTGCA
  TTTAAAGAGGGATTAAAGATCCCACCTCCAGCTATGAATGAAATAATTTTGGGAGCTAATCAAGATGTCA
  GACAGGTTTTACACAATCTCAGTATGTGGTGTGCACAGAGTAAGGTCCTAACCTATGACCAAGCCAAGGC
  TGATTCTCAGAGGGCCAAGAAGGACATCAGATTGGGCCCATTTGATGTCACCCGGAAAGTGTTTGCAGCT
  GGAGAGGAGACTGCACACATGTCACTGATGGACAAATCGGATCTCTTTTTCCATGACTATTCCATTGCAC
  CCCTCTTTGTCCAGGAGAACTACCTCCATGTAAAGCCTGTGGCTGCAGGGGGTGACATGAAAAAGCACTT
  GATGCTTTTAAGCCGGGCAGCAGATAGCATATGTGACGGTGACTTAGTGGACAATCAGATCCGGAGTAAG
  CAAAACTGGAGTCTTCTGCCCACACAGGCCATTTACGCCAGCGTTGTTCCTGGAGAGTTGATGAGGGGCT
  ACATGTCTCAGTTTCCCAGCTTCCCAAGCTGGCTGGGAAAGCACTCGTCTATGGGCAAGCATGATCGGAC
  TGTTCAGGACCTGTCCTTGCACATGAGTCTTAGAACTTACTCCAGCAAAAGGACAGTGAACATGGATTAC
  CTGTCACACATCAGAGACGCACTTGTACGACCCTTGACCTCACAAGGAGTAGAAGGGGTTCAGCGTGTTG
  TCACACTTATGGATACATACTATTTGGTGAAAGAAGATTTTGACAACATCATGGAAGTTAGCAGCTGGGG
  GGGCAAGCCCAGTACCTTTTCCAAGCTGGACCCCAAGGTGAAAGCAGCCTTCACAAGAGCCTATAATAAG
  GAGGCCCACCTGACCCCATACTCACTTCAGGTGGTAAAGGCATCGAGGCCCAGCATAGGTCCAGCACTGG
  ATTCTGAGTACAATGAAGAGTCACAGGAAGATGCCACTCAGTCTGATGAGAAAGAGCAGGATGCCCTAGA
  GACTGATGCCATGATCAAGCAGAAAAAGACGAGATCTTCAAAGCCTTCAAAATCAGAAAAAGAGAAGGAG
  TCCAAAAAAGGAAAAGGAAAAAATTCGAAGAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_053547
• Insert Size: 3396 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053547.1, NP_445999.1
• RefSeq Size: 4741 bp
• RefSeq ORF: 3396 bp
• Locus ID: 89809
• Cytogenetics: 14p11
• Gene Summary: transcriptional repressor that regulates transcription of the vasoactive intestinal peptide receptor gene [RGD, Feb 2006]