Dcun1d5 (NM_001009696) Rat Untagged Clone

CAT#: RN212059

Dcun1d5 (untagged ORF) - Rat DCN1, defective in cullin neddylation 1, domain containing 5 (S. cerevisiae) (Dcun1d5), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Dcun1d5
• Synonyms: RGD1307008
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN212059 representing NM_001009696
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGTGAAGAAGAAGAGAAAAGCTCCCGGAGTGGCAGCGGCGGTCGCGGAAGACGCGGGCCTCAAAA
  AGTGTAAAATCTCCAGCTATTGCAGATCCCAGCCTCCTGCTAGACTCATCAGTGGAGAGGAAGATTTTTC
  AAGAAAGAAGTGCCTGGCTTGGTTTTATGAGTATGCAGGTCCTGATGAAGTTGTAGGGCCAGAAGGAATG
  GAAAAGTTTTGTGAAGACATTGGTGTTGAACCTGAAAATATTATTATGTTAGTTTTAGCGTGGAAATTGG
  AGGCTGAAAGCATGGGATTTTTTACCAAGGAAGAATGGTTAAAGGGAATGACTTCATTACAGTGTGACTG
  CACAGAAAAGTTACAAAGCAGGTTTGACTTCCTGCGCTCACAGCTGAACGACATCTCGTCATTTAAGAAC
  ATCTACAGATACGCCTTTGATTTTGCAAGGGATAAAGATCAGAGAAGCCTTGATATTGATACTGCTAAGT
  CTATGCTAGCTCTGCTGCTTGGGAGGACGTGGCCACTTTTTTCAGTATTTTACCAGTACCTGGAGCAATC
  AAAGTATCGTGTTATGAACAAAGATCAGTGGTACAATGTATTAGAATTCAGCAGAACAGTTCATGCCGAT
  CTTAGTAACTACGATGAAGACGGCGCTTGGCCTGTTCTTCTTGATGAGTTTGTTGAGTGGCAAAAAATCC
  GTCAAACATCATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001009696
• Insert Size: 714 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009696.1, NP_001009696.1
• RefSeq Size: 1230 bp
• RefSeq ORF: 714 bp
• Locus ID: 315405
• UniProt ID: Q5PPL2
• Cytogenetics: 8q11
• Gene Summary: Contributes to the neddylation of all cullins by transfering NEDD8 from N-terminally acetylated NEDD8-conjugating E2s enzyme to different cullin C-terminal domain-RBX complexes which is necessary for the activation of cullin-RING E3 ubiquitin ligases (CRLs). May play a role in DNA damage response and may participate to cell proliferation and anchorage-independent cell growth.[UniProtKB/Swiss-Prot Function]