Adcyap1r1 (NM_133511) Rat Untagged Clone

CAT#: RN211909

Adcyap1r1 (untagged ORF) - Rat adenylate cyclase activating polypeptide 1 (pituitary) receptor type I (Adcyap1r1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Adcyap1r1
• Synonyms: PAC1-R; PACAP-R1; PACAP-R1A; PACAPR1; PACAPR1A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN211909 representing NM_133511
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGAGTCCTGCAGCTCTCCCTGACTGCTCTCCTGCTGCCTGTGGCTATTGCTATGCACTCTGACT
  GCATCTTCAAGAAGGAGCAAGCCATGTGCCTGGAGAGGATCCAGAGGGCCAACGACCTGATGGGACTAAA
  CGAGTCTTCCCCAGGTTGCCCTGGCATGTGGGACAATATCACATGTTGGAAGCCAGCTCAAGTAGGTGAG
  ATGGTCCTTGTAAGCTGCCCTGAGGTCTTCCGGATCTTCAACCCGGACCAAGTCTGGATGACAGAAACCA
  TAGGAGATTCTGGTTTTGCCGATAGTAATTCCTTGGAGATCACAGACATGGGGGTCGTGGGCCGGAACTG
  CACAGAGGACGGCTGGTCGGAGCCCTTCCCCCACTACTTCGATGCTTGTGGGTTTGATGATTATGAGCCT
  GAGTCTGGAGATCAGGATTATTACTACCTGTCGGTGAAGGCTCTCTACACAGTCGGCTACAGCACTTCCC
  TCGCCACCCTCACTACTGCCATGGTCATCTTGTGCCGCTTCCGGAAGCTGCATTGCACTCGCAACTTCAT
  CCACATGAACCTGTTTGTATCCTTCATGCTGAGGGCTATCTCCGTCTTCATCAAGGACTGGATCTTGTAC
  GCCGAGCAGGACAGCAGTCACTGCTTCGTTTCCACCGTGGAGTGCAAAGCTGTCATGGTTTTCTTCCACT
  ACTGCGTGGTGTCCAACTACTTCTGGCTGTTCATTGAAGGCCTGTACCTCTTTACACTGCTGGTGGAGAC
  CTTCTTCCCTGAGAGGAGATATTTCTACTGGTACACCATCATCGGCTGGGGGACACCTACTGTGTGTGTA
  ACAGTGTGGGCTGTGCTGAGGCTCTATTTTGATGATGCAGGATGCTGGGATATGAATGACAGCACAGCTC
  TGTGGTGGGTGATCAAAGGCCCCGTGGTTGGCTCTATAATGGTTAACTTTGTGCTTTTCATCGGCATCAT
  CATCATCCTTGTACAGAAGCTGCAGTCCCCAGACATGGGAGGCAACGAGTCCAGCATCTACTTCAGCTGC
  GTGCAGAAATGCTACTGCAAGCCACAGCGGGCTCAGCAGCACTCTTGCAAGATGTCAGAACTATCCACCA
  TTACTCTACGGCTGGCCCGCTCCACCCTACTGCTCATCCCACTCTTCGGAATCCACTACACAGTATTCGC
  CTTCTCTCCAGAGAACGTCAGCAAGAGGGAAAGACTTGTGTTTGAGCTTGGGCTGGGCTCCTTCCAGGGC
  TTTGTGGTGGCTGTACTCTACTGCTTCTTGAATGGGGAGGTACAGGCAGAGATTAAGAGGAAATGGAGGA
  GCTGGAAGGTGAACCGTTACTTCACTATGGACTTCAAGCACCGGCACCCGTCCCTGGCCAGCAGTGGAGT
  AAATGGGGGAACCCAGCTGTCCATCCTGAGCAAGAGCAGCTCCCAGCTCCGCATGTCCAGCCTCCCGGCC
  GACAACTTGGCCACCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133511
• Insert Size: 1488 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133511.2, NP_598195.1
• RefSeq Size: 6235 bp
• RefSeq ORF: 1488 bp
• Locus ID: 24167
• UniProt ID: P32215
• Cytogenetics: 4q24
• Gene Summary: regulates neural precursor proliferation; mediates inhibitory signaling for Shh-induced cerebellar granule precursor cell proliferation [RGD, Feb 2006]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant (1). The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.