Arhgap24 (NM_001012032) Rat Untagged Clone

CAT#: RN211687

Arhgap24 (untagged ORF) - Rat Rho GTPase activating protein 24 (Arhgap24), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Arhgap24
• Synonyms: DR-NR#2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN211687 representing NM_001012032
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGAAGACCGAAATTCGGGGGGACGCCCTTCAGGTGCCTTAGCATCAACTCCTTTCATCCCCAAAA
  CTACATACAGAAGAATCAAACGGTGCTTTAGTTTCCGGAAAGGTATTTTTGGACAGAAACTGGAGGACAC
  GGTCCGATATGAGAAGAGATACGGGAACCGGCTGGCGCCCATGCTGGTGGAGCAGTGCGTGGACTTCATC
  CGACAGCGGGGGCTGAAGGAAGAGGGCCTCTTCCGACTGCCCGGCCAGGCAAACCTTGTCAAGGAGCTCC
  AGGATGCATTCGACTGTGGAGAGAAGCCCTCCTTCGACAGCAACACAGACGTCCACACTGTGGCATCCCT
  CCTGAAACTGTATCTCCGAGAACTTCCAGAGCCGGTCGTTCCTTACGCAAAGTACGAAGACTTTTTGTCC
  TGTGCCACGCTGCTCAGCAAGGAGGAAGAAGCTGGCGTGAAGGAGCTAACGAAACAAGTGAAGAGTTTGC
  CAGTGGTTAACTACAACCTCCTGAAGTACATTTGCAGGTTCCTGGATGAAGTGCAGTCCTATTCGGGAGT
  TAACAAGATGAGCGCACAGAACTTGGCAACTGTCTTCGGTCCTAACATCCTGCGCCCAAAGGTGGAAGAT
  CCTTTAACCATCATGGAGGGCACTGTGGTGGTCCAGCAGTTAATGTCAGTGATGATCAGCAAACATGATC
  GCCTCTTCCCCAAAGACACAGAGCCACAAAGCAAGCCCCAAGAAGGACCGAACAGCAATAACAACGATGG
  TCATAAGAAAGTCACCATGGGTCAGTTACAGAACAAAGAAAACAACAATACCAAGGAGAGTCCGGTCAGA
  CGGTGCTCTTGGGACAAGCCTGAATCCCCCCAGAGAAGCAGCATGGACAACGGATCCCCCACAGCTCTAT
  CAGGGAGCAAAACCAACAGCCCACGGAACAGTATCCACAAGCTGGATGTCTCCAGGAGCCCGCCTCTCAC
  GGTCAAAAAGAACCCAGCCTTCAATAAGGGCAGCGGGATCGTCACCAATGGGTCCTTCAGCAGCAGCAAT
  GCAGAAGGTGTGGAGAAAACCCAAACCACTCCTAATGGGAGTTTACAAGCCAGAAGGACCTCTTCCCTGA
  AGAGTTCTGGTACCAAAATGGGTACTCATAGCGTACAGAATGGCACCGTGCGCATGGGTATTTTGAACAC
  TGACACACTAGGGAATTCCTTGAATGGCCGGAGCATGAGTTGGCTGCCCAATGGCTACGTGACCCTGAGA
  GATAACAAGCAGAAGGAGCCCGCGGGAGAGTCAGGCCAGCACAACAGACTCTCCACCTACGACAACGTCC
  ACCAGCAGTTCTCCTTGATGAACCTCGACGACAAGCACAGCGTGGACAGTGCCACGTGGTCCACCTCCTC
  CTGTGAAATCTCCCTCCCCGAGAACTCCAACTCCTGCCGCTCCTCTACCACTACGTGCCCGGAGCAAGAC
  TTCTACGGCGGGAACTTTGAGGACCCTGTTTTAGACGGCCCGCCTCAGGATGACCTTTCTCATCCTGGGG
  ACTATGAGAACAAAAGCGACCGCAGGAGCGTGGGGGGCCGAAGCAGCCGTGCCACGAGCAGCAGCGACAA
  CAGCGAGACGTTCGTCGGTAACACCAGCAGCAACCACAGTGCACTTCACAGTTTAGTCTCCAGCCTGAAG
  CAGGAGATGACCAAACAGAAGATCGAGTATGAGTCCAGGATAAAAAGCTTAGAACAGCGGAACTTGACTT
  TGGAAACGGAAATGCTGAACCTCCATGACGAACTGGATCAAGAGAGGAAAAAGTTCACCATGATAGAAAT
  CAAGATGAGAAACGCCGAGCGCGCGAAAGAGGATGCTGAGAAGAGGAACGACATGCTCCAGAAGGAAATG
  GAGCAGTTCTTCTCCACATTCGGAGACCTGACGGTGGAACCCAGGAGAAGCGAGAGGGGAAACACAATCT
  GGATCCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001012032
• Insert Size: 1971 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012032.1, NP_001012032.1
• RefSeq Size: 2728 bp
• RefSeq ORF: 1971 bp
• Locus ID: 305156
• UniProt ID: Q5U2Z7
• Cytogenetics: 14p22
• Gene Summary: Rho GTPase-activating protein involved in cell polarity, cell morphology and cytoskeletal organization. Acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. Controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. Able to suppress RAC1 and CDC42 activity in vitro. Overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis (By similarity).[UniProtKB/Swiss-Prot Function]